中华眼底病杂志
中華眼底病雜誌
중화안저병잡지
CHINESE JOURNAL OF OCULAR FUNDUS DISEASES
2011年
3期
271-274
,共4页
梁小玲%周焕娇%杨诚%孙刚%张熙芳%魏丽清
樑小玲%週煥嬌%楊誠%孫剛%張熙芳%魏麗清
량소령%주환교%양성%손강%장희방%위려청
视网膜疾病/药物疗法%川芎嗪/治疗应用%细胞凋亡/药物作用%动物实验
視網膜疾病/藥物療法%川芎嗪/治療應用%細胞凋亡/藥物作用%動物實驗
시망막질병/약물요법%천궁진/치료응용%세포조망/약물작용%동물실험
Retinal diseases/drug therapy%Tetramethylpyrazine/therapeutic use%Apoptosis/drug effects%Animal experimentation
目的 观察川芎嗪(TMP)对氧诱导视网膜病变(OIR)中视网膜神经细胞凋亡的抑制作用.方法 48只C57BL/6新生幼鼠随机分为正常对照组、OIR对照组及OIR药物组,分别为18、18、12只.正常对照组在正常空气环境中饲养.OIR对照组、OIR药物组幼鼠于出生后7 d置于含氧体积分数(75±3)%的氧气箱中连续生活5 d;出生后12 d,幼鼠回到正常空气环境中饲养,建立OIR模型.出生后12～16d,OIR药物组按200 mg/kg的剂量腹腔注射TMP,1次/d;正常对照组、OIR对照组同时注射相同体积的含0.1%二甲基亚砜(DMSO)的生理盐水.出生后12、14、17 d,摘除幼鼠眼球作石蜡切片并行苏木精-伊红(HE)和脱氧核苷酸末端转移酶介导缺口末端标记(TUNEL)染色.观察视网膜无灌注区形态学变化,检测视网膜神经细胞凋亡数量.结果 正常对照组幼鼠视网膜各层结构清晰.出生后12 d,OIR对照组幼鼠视网膜内核层(INL)可见少量染色质凝聚的细胞核.出生后14 d,OIR对照组幼鼠视网膜INL可见大量染色质凝聚的细胞核,OIR药物组幼鼠视网膜INL均可见少量染色质凝聚的细胞核.出生后17 d,OIR对照组幼鼠视网膜INL、内丛状层(IPL)和外丛状层(OPL)厚度变薄;OIR药物组幼鼠视网膜中周部INL、IPL和OPL厚度较正常对照组薄,较OIR对照组厚.出生后12 d,OIR对照组幼鼠视网膜TUNEL阳性细胞数量为正常对照组的6倍,差异有统计学意义(t=9.432,P＜0.001).出生后14 d,3组幼鼠视网膜TUNEL阳性细胞数量比较,差异有统计学意义(F=587.217,P＜0.001);OIR对照组幼鼠视网膜TUNEL阳性细胞数量为正常对照组的28倍,差异有统计学意义(t=49.813,P＜0.001);OIR药物组幼鼠视网膜TUNEL阳性细胞数量较OIR对照组减少了82.3%,差异有统计学意义(t=42.434,P＜0.001).出生后17 d,3组幼鼠视网膜TUNEL阳性细胞数量比较,差异无统计学意义(F=587.217,P>0.05).结论 TMP能明显抑制OIR中视网膜神经细胞凋亡.
目的 觀察川芎嗪(TMP)對氧誘導視網膜病變(OIR)中視網膜神經細胞凋亡的抑製作用.方法 48隻C57BL/6新生幼鼠隨機分為正常對照組、OIR對照組及OIR藥物組,分彆為18、18、12隻.正常對照組在正常空氣環境中飼養.OIR對照組、OIR藥物組幼鼠于齣生後7 d置于含氧體積分數(75±3)%的氧氣箱中連續生活5 d;齣生後12 d,幼鼠迴到正常空氣環境中飼養,建立OIR模型.齣生後12～16d,OIR藥物組按200 mg/kg的劑量腹腔註射TMP,1次/d;正常對照組、OIR對照組同時註射相同體積的含0.1%二甲基亞砜(DMSO)的生理鹽水.齣生後12、14、17 d,摘除幼鼠眼毬作石蠟切片併行囌木精-伊紅(HE)和脫氧覈苷痠末耑轉移酶介導缺口末耑標記(TUNEL)染色.觀察視網膜無灌註區形態學變化,檢測視網膜神經細胞凋亡數量.結果 正常對照組幼鼠視網膜各層結構清晰.齣生後12 d,OIR對照組幼鼠視網膜內覈層(INL)可見少量染色質凝聚的細胞覈.齣生後14 d,OIR對照組幼鼠視網膜INL可見大量染色質凝聚的細胞覈,OIR藥物組幼鼠視網膜INL均可見少量染色質凝聚的細胞覈.齣生後17 d,OIR對照組幼鼠視網膜INL、內叢狀層(IPL)和外叢狀層(OPL)厚度變薄;OIR藥物組幼鼠視網膜中週部INL、IPL和OPL厚度較正常對照組薄,較OIR對照組厚.齣生後12 d,OIR對照組幼鼠視網膜TUNEL暘性細胞數量為正常對照組的6倍,差異有統計學意義(t=9.432,P＜0.001).齣生後14 d,3組幼鼠視網膜TUNEL暘性細胞數量比較,差異有統計學意義(F=587.217,P＜0.001);OIR對照組幼鼠視網膜TUNEL暘性細胞數量為正常對照組的28倍,差異有統計學意義(t=49.813,P＜0.001);OIR藥物組幼鼠視網膜TUNEL暘性細胞數量較OIR對照組減少瞭82.3%,差異有統計學意義(t=42.434,P＜0.001).齣生後17 d,3組幼鼠視網膜TUNEL暘性細胞數量比較,差異無統計學意義(F=587.217,P>0.05).結論 TMP能明顯抑製OIR中視網膜神經細胞凋亡.
목적 관찰천궁진(TMP)대양유도시망막병변(OIR)중시망막신경세포조망적억제작용.방법 48지C57BL/6신생유서수궤분위정상대조조、OIR대조조급OIR약물조,분별위18、18、12지.정상대조조재정상공기배경중사양.OIR대조조、OIR약물조유서우출생후7 d치우함양체적분수(75±3)%적양기상중련속생활5 d;출생후12 d,유서회도정상공기배경중사양,건립OIR모형.출생후12～16d,OIR약물조안200 mg/kg적제량복강주사TMP,1차/d;정상대조조、OIR대조조동시주사상동체적적함0.1%이갑기아풍(DMSO)적생리염수.출생후12、14、17 d,적제유서안구작석사절편병행소목정-이홍(HE)화탈양핵감산말단전이매개도결구말단표기(TUNEL)염색.관찰시망막무관주구형태학변화,검측시망막신경세포조망수량.결과 정상대조조유서시망막각층결구청석.출생후12 d,OIR대조조유서시망막내핵층(INL)가견소량염색질응취적세포핵.출생후14 d,OIR대조조유서시망막INL가견대량염색질응취적세포핵,OIR약물조유서시망막INL균가견소량염색질응취적세포핵.출생후17 d,OIR대조조유서시망막INL、내총상층(IPL)화외총상층(OPL)후도변박;OIR약물조유서시망막중주부INL、IPL화OPL후도교정상대조조박,교OIR대조조후.출생후12 d,OIR대조조유서시망막TUNEL양성세포수량위정상대조조적6배,차이유통계학의의(t=9.432,P＜0.001).출생후14 d,3조유서시망막TUNEL양성세포수량비교,차이유통계학의의(F=587.217,P＜0.001);OIR대조조유서시망막TUNEL양성세포수량위정상대조조적28배,차이유통계학의의(t=49.813,P＜0.001);OIR약물조유서시망막TUNEL양성세포수량교OIR대조조감소료82.3%,차이유통계학의의(t=42.434,P＜0.001).출생후17 d,3조유서시망막TUNEL양성세포수량비교,차이무통계학의의(F=587.217,P>0.05).결론 TMP능명현억제OIR중시망막신경세포조망.
Objective To observe the inhibitory effect of tetramethylpyrazine(TMP)on apoptosis of retinal neurons in oxygen-induced retinopathy(OIR).Methods 48 C57BL/6 mice were randomly divided into normal control group(n=18),OIR control group(n=18)and OIR TMP group(n=12).The mice of normal control group were raised in room air.From the postnatal day 7(P7),mice of the other two groups mice of OIR TMP group received intraperitoneal injection of TMP(200 mg/kg)once a day from P12 to P16.meanwhile the mice of normal control group and OIR control group were iniected with the same volume of normal saline containing of 0.1% DMSO.At P12,P14 and P17,the morphologic changes in retinal avascular zone and the number of retinal apoptotic cell were observed by HE staining and TUNEL assay.Results At P1 2.there were a few of chromatin condensation and pycnic nuclei in the inner nuclear layer (INL)of OIR control group.At P14,a great quantity(OIR control group)or some(OID TMP treated group)chromatin condensation and pycnic nuclei in the central INL were observed.At P17,the thickness of INL,inner plexiform layer(IPL)and outer plexiform layer(OPL)in the OIR control group were reduced;the thickness of INL,IPL and OPL in the OIR TMP group weas thinner than those in the normal control group and thicker than those in the OIR control group.At P12,the TUNEL-positive cells in the OIR control group was 6 times of the normal eontrol group(F=587.217,P＜0.001).At P14,the difference of TUNEL-positive cells in three groups was significant(F=587.217,P＜0.001);the TUNEL-positive cells in the OIR control group was 28 times of the normal control group(t=49.813,P＜0.001);the TUNEL-positive cells in the OIR TMP group has reduced 50% compared with the OIR controI group(t=42.434,P＜0.00 1).At P17,there was no significant difference in TUNEL-positive cells among the three groups (F=587.217,P>0.05).Conclusions TMP can inhibit apoptosis of retinal cells in OIR significantly.
