CAJ | 학술논문

目的研究A、C群脑膜炎球菌结合疫苗的免疫原性,包括剂量、免疫记忆及抗原相容性.方法将不同剂量的A、C群脑膜炎球菌结合疫苗分别免疫小鼠,末次免疫后14 d采集血清测定血清抗体滴度,确定最佳免疫剂量后,再分别以单价A群和C群脑膜炎球菌结合疫苗及二价A、C群脑膜炎球菌结合疫苗同时对小鼠分别进行1、2、3针免疫,考察其针次效应和抗原相容性.结果 A、C群脑膜炎球菌结合疫苗中的A抗原和C抗原均以1.25 μg剂量免疫小鼠时,其免疫原性较好.A、C群脑膜炎球菌结合疫苗能够诱导小鼠产生免疫回忆应答,且在二价结合疫苗中的A群耦联物和C群耦联物配伍后无干扰作用,抗原相容性良好.结论 A、C群脑膜炎球菌结合疫苗具有较好的免疫原性和明显的免疫回忆应答,抗原相容性良好.
목적 연구A、C군뇌막염구균결합역묘적면역원성,포괄제량、면역기억급항원상용성.방법 장불동제량적A、C군뇌막염구균결합역묘분별면역소서,말차면역후14 d채집혈청측정혈청항체적도,학정최가면역제량후,재분별이단개A군화C군뇌막염구균결합역묘급이개A、C군뇌막염구균결합역묘동시대소서분별진행1、2、3침면역,고찰기침차효응화항원상용성.결과 A、C군뇌막염구균결합역묘중적A항원화C항원균이1.25 μg제량면역소서시,기면역원성교호.A、C군뇌막염구균결합역묘능구유도소서산생면역회억응답,차재이개결합역묘중적A군우련물화C군우련물배오후무간우작용,항원상용성량호.결론 A、C군뇌막염구균결합역묘구유교호적면역원성화명현적면역회억응답,항원상용성량호.
Objective To investigate the immunogenicity of group A plus group C meningococeal glycoconjugate vaccine,namely dosage,immune memory and compatibility. Methods The mice were injected with group A plus group C meningococcal glycoconjugate vaccine with different dosages. Blood samples were taken on the 14th day after the last injection for testing the antibodies against polysaccharide A and C. After the optimal immunization dosage had been decided,the mice were inoculated separately with the monovalent group A and the monovalent group C and the bivalent group A plus group C glycoconjugate vaccine with one,two or three injections for observation of the effectiveness of different injections and the compatibilities. Results The dosage of 1.25 μg of each polysaccharide of group A and group C in bivalent glycoconjugate vaccine appears to be immunologically optimal to vaccinate the mice. Immunological memory could be induced in mice inoculated with the glycoconjugate vaccine,and the antigenic immunogenicity of the group A component and group C component in the formulation of group A plus group C meningococcal glycoconjugate vaccine was not affected. Conclusion Group A plus group C meningococcal glycoconjugate vaccine have good immunogenicity,immune memory and compatibility.