CAJ | 학술논문

对番茄下胚轴、子叶、叶片、叶柄不同类型外植体离体培养中有关细胞启动、分裂、分化以及器官发生作了细胞组织学观察，结果表明：番茄不同类型外植体在同样的培养条件下，愈伤组织生长表现明显差异：下胚轴、子叶诱导产生愈伤组织时，细胞启动早，生长快，分裂方式基本为无丝分裂；下胚轴诱导愈伤组织形成时，细胞不规则的无丝分裂少于子叶，故下胚轴离体培养得到正常芽的比例高于子叶；番茄离体培养中不定芽通常发生在愈伤组织的周边区，也可起源于维管组织结节周围，形成层状细胞。不定根则由茎中柱鞘处发生。
대번가하배축、자협、협편、협병불동류형외식체리체배양중유관세포계동、분렬、분화이급기관발생작료세포조직학관찰，결과표명：번가불동류형외식체재동양적배양조건하，유상조직생장표현명현차이：하배축、자협유도산생유상조직시，세포계동조，생장쾌，분렬방식기본위무사분렬；하배축유도유상조직형성시，세포불규칙적무사분렬소우자협，고하배축리체배양득도정상아적비례고우자협；번가리체배양중불정아통상발생재유상조직적주변구，야가기원우유관조직결절주위，형성층상세포。불정근칙유경중주초처발생。
Hypocotyl, cotyledon, leaf blade and leaf petiole explants of tomato were cultured on MS medium supplemented with 2.0mg/L BA and 0.2mg/L IAA for induction of callus and bud formation. Histological observations on callus and bud formation were carried out. The following results were obtained: there were significant difference in callus induction and growth among above-mentioned 3 kinds of explants, calli from hypocotyl and cotyledon explants were induced earlier than others, and grew faster than others. Buds differentiated from calli were adventitious buds. Adventitious buds were generally formed on the peripheral parts of the calli, also originated from the cambium-like cells surrounded by the vascular nodules. Adventitious roots originated from the pericycle. The caryological observation on callus paraffin section showed that, in the course of callus formation from cotyledon there were more kinds of nuclear division modes than hypocotyl calli, but all these were of amitosis.