CAJ | 학술논문

目的:考察不同脂质体材料对阳离子脂质体细胞转染率和脂质体膜流动性的影响.方法:合成美国FDA认可应用于人体给药的阳离子材料3β-[N-(N′, N′- 二甲基胺乙基)胺基甲酰基]胆固醇(3β[N-(N′, N′-dimethylaminoethane) carbamoyl] cholesterol,DC-chol);以荧光素钠(fluorescein sodium,SF)为荧光标记物,二棕榈酰磷脂酰胆碱(dipalmitoylphosphatidylcholine,DPPC)、二油酰磷脂酰乙醇胺(dioleoylphosphatidylethanolamine,DOPE)和胆固醇(cholesterol,ch)为辅佐膜材,分别制备DPPC/ch/DC-chol阳离子脂质体、DOPE/ch/DC-chol阳离子脂质体及大豆甾醇糖苷(soybean-derived sterylglucoside,SG)或聚乙二醇-二硬脂酰磷脂酰乙醇胺(polyethylene glycol-distearoylphosphatidylethanolamine,PEG-DSPE)表面修饰的阳离子脂质体,并制备DPPC/ch组成的中性脂质体.以HepG2 2.2.15 为细胞模型观察不同脂质体处方对细胞转染率的影响;通过荧光偏振法考察不同脂质材料对脂质体膜流动性的影响.结果:DC-chol能显著增加脂质体对SF的包封率(从0.64%到的74.84%)、细胞转染率(从1.83%到32.29%)及脂质体膜的流动性.在阳离子脂质体处方中加入SG能显著提高阳离子脂质体的包封率(从52.60%到63.37%)、细胞转染率(从20.18%到47.48%)及脂质体膜的流动性;PEG-DSPE可促进阳离子脂质体的细胞转染(从20.18%到28.17%).结论:DC-chol和SG能显著提高脂质体的细胞转染率和脂质体膜的流动性;PEG-DSPE能促进阳离子脂质体的细胞转染.
목적:고찰불동지질체재료대양리자지질체세포전염솔화지질체막류동성적영향.방법:합성미국FDA인가응용우인체급약적양리자재료3β-[N-(N′, N′- 이갑기알을기)알기갑선기]담고순(3β[N-(N′, N′-dimethylaminoethane) carbamoyl] cholesterol,DC-chol);이형광소납(fluorescein sodium,SF)위형광표기물,이종려선린지선담감(dipalmitoylphosphatidylcholine,DPPC)、이유선린지선을순알(dioleoylphosphatidylethanolamine,DOPE)화담고순(cholesterol,ch)위보좌막재,분별제비DPPC/ch/DC-chol양리자지질체、DOPE/ch/DC-chol양리자지질체급대두치순당감(soybean-derived sterylglucoside,SG)혹취을이순-이경지선린지선을순알(polyethylene glycol-distearoylphosphatidylethanolamine,PEG-DSPE)표면수식적양리자지질체,병제비DPPC/ch조성적중성지질체.이HepG2 2.2.15 위세포모형관찰불동지질체처방대세포전염솔적영향;통과형광편진법고찰불동지질재료대지질체막류동성적영향.결과:DC-chol능현저증가지질체대SF적포봉솔(종0.64%도적74.84%)、세포전염솔(종1.83%도32.29%)급지질체막적류동성.재양리자지질체처방중가입SG능현저제고양리자지질체적포봉솔(종52.60%도63.37%)、세포전염솔(종20.18%도47.48%)급지질체막적류동성;PEG-DSPE가촉진양리자지질체적세포전염(종20.18%도28.17%).결론:DC-chol화SG능현저제고지질체적세포전염솔화지질체막적류동성;PEG-DSPE능촉진양리자지질체적세포전염.