CAJ | 학술논문

目的从细胞受体和免疫分子水平探讨T2DM内毒素性肺损伤肺局部防御功能减弱的机制.方法雄性Wistar大鼠高糖高脂饲料喂养,小剂量链脲佐菌素(STZ)注射建立T2DM大鼠模型.经尾静脉注射脂多糖(LPS)建立内毒素血症/急性肺损伤大鼠模型.肺泡巨噬细胞(AM)表面CD14表达测定采用免疫组化法,并进行计算机图像分析.放免法检测血清及肺组织匀浆液中TNF-α、IL-6的水平.结果T2DM大鼠AM表面CD14表达和血清TNF-α、IL-6水平明显高于正常对照大鼠(P＜0.05).而经LPS激活后,T2DM及正常对照大鼠AM表面CD14表达及血清、肺组织匀浆液中TNF-α、II-6水平明显增高(P＜0.05),但T2DM组上述指标低于正常对照组,差异有统计学意义(P＜0.01).结论T2DM肺组织的局部防御功能减弱可能与AM经LPS激活后CD14分子上调表达减弱及分泌TNF-α、IL-6能力下降有关.
목적종세포수체화면역분자수평탐토T2DM내독소성폐손상폐국부방어공능감약적궤제.방법웅성Wistar대서고당고지사료위양,소제량련뇨좌균소(STZ)주사건립T2DM대서모형.경미정맥주사지다당(LPS)건립내독소혈증/급성폐손상대서모형.폐포거서세포(AM)표면CD14표체측정채용면역조화법,병진행계산궤도상분석.방면법검측혈청급폐조직균장액중TNF-α、IL-6적수평.결과T2DM대서AM표면CD14표체화혈청TNF-α、IL-6수평명현고우정상대조대서(P＜0.05).이경LPS격활후,T2DM급정상대조대서AM표면CD14표체급혈청、폐조직균장액중TNF-α、II-6수평명현증고(P＜0.05),단T2DM조상술지표저우정상대조조,차이유통계학의의(P＜0.01).결론T2DM폐조직적국부방어공능감약가능여AM경LPS격활후CD14분자상조표체감약급분비TNF-α、IL-6능력하강유관.
Objective To investigate the mechanism for weakened pulmonary local immune defensive function at levels of cell receptor and immunomolecule in type 2 diabetes mellitus(T2DM) rats with acute lung injury induced by lipopolysaccharide(LPS).Methods Wistar rats were fed with the diets rich in highsucrose and highlipid.T2DM model was developed by intraperitoneal injection with streptozotocin 35 mg/kg of body weight 1 month after the diet treatment.Murine model of endotoxemia/ acute lung injury was produced by injection LPS(1 mg/kg of body weight) via tail vein.The expression of CD14 on alveolar macrophage(AM) surface was assayed by immunohistochemistry and then analyzed with computer imaging system.Radioimmunoassay was used to determine the levels of TNF-α and IL-6 in serum and pulmonary homogenates.Results The expression of CD14 on AM surface and the levels of TNF-α and IL-6 in serum in T2DM rats without LPS challenge were significantly higher than those in normal control rats(P<0.05).There was markedly increased average absorbance(A) value of CD14 and the levels of TNF-α and IL-6 in serum and pulmonary homogenates in both normal control rats and T2DM rats after LPS challenge(P<0.01),but the parameters mentioned above in T2DM rats were significantly lower than those in normal control rats after LPS challenge(P<0.01).Conclusions The expression of CD14 on AM surface and the secretion of TNF-α and IL-6 after LPS challenge are severely decreased in T2DM rats.This might be one of important mechanisms for weakened pulmonary local immune defensive function in T2DM.