CAJ | 학술논문

利用聚丙烯酰胺凝胶电泳分析了泥蚶(Tegillarca granosa)能量代谢相关的10种同工酶.酯酶和α-淀粉酶是同能量吸收有关的酶,主要分布于消化腺里.苹果酸脱氢酶、苹果酸酶、异柠檬酸脱氢酶、琥珀酸脱氢酶、醇脱氢酶、乳酸脱氢酶、6-磷酸葡萄糖脱氢酶和三磷酸腺苷激酶是与能量代谢相关的酶.泥蚶的能量供应有3种途径:有氧呼吸、无氧酵解和磷酸戊糖途径.从泥蚶体内含有很高活性的6-磷酸葡萄糖脱氢酶可知,磷酸戊糖途径是泥蚶糖代谢的主要途径,NADP为供能体.同其它贝类相比,泥蚶的三磷酸腺苷激酶的活性很低,这与它们的埋栖生活不活跃运动的习性是一致的.
이용취병희선알응효전영분석료니감(Tegillarca granosa)능량대사상관적10충동공매.지매화α-정분매시동능량흡수유관적매,주요분포우소화선리.평과산탈경매、평과산매、이저몽산탈경매、호박산탈경매、순탈경매、유산탈경매、6-린산포도당탈경매화삼린산선감격매시여능량대사상관적매.니감적능량공응유3충도경:유양호흡、무양효해화린산무당도경.종니감체내함유흔고활성적6-린산포도당탈경매가지,린산무당도경시니감당대사적주요도경,NADP위공능체.동기타패류상비,니감적삼린산선감격매적활성흔저,저여타문적매서생활불활약운동적습성시일치적.
Ten isozymes connected with energy metabolism in Tegillarca granosa were analysed by vertical polyacrylamide gel electrophoresis. Esterase and α-amylase are isozymes related with energy intake, their activities were high in the digestive gland.Malate dehydrogenase, malic enzyme, isocitrate dehydrogenase, succinate dehydrogenase, alcohol dehydrogenase, lactate dehydrogenase, 6-phosphogluconate dehydrogenase (G-6-PDH) and adenosine triphosphatase (ATPase) are isozymes related with energy metabolism. The main energy supply of T. granosa comes from aerobic respiration, anaerobic metabolism and phosphopentase pathway plays the auxiliary role in the energy metabolism. The high activity of G-6-PDH in T. granosa might mean a considerable proportion of carbohydrates metabolized through this pathway. This reaction could provide abundant NADP for the metabolism in T.granosa. Compared with other shellfish, T. granosa had lower activity of ATPase, which might have some relationships with the amnicolous life and weak moving ability of this animal.