CAJ | 학술논문

Hedgehog信号通路在胚胎发育过程中发挥着重要作用,同时与多种肿瘤的发生密切相关.Rab23蛋白在Hedgehog信号通路中扮演着十分重要的角色.目前关于文昌鱼Rab23同源基因的研究仅局限于佛罗里达文昌鱼(Branchiostoma floridae)基因组中的注释.文章首次克隆了中国文昌鱼(Branchiostoma belcheri)Rab23b基因(AmphiRab23b)cDNA全序列,对其演译的蛋白序列进行了序列比对,进化树分析以及基因时空表达分析.研究结果显示,文昌鱼AmphiRab23b基因的cDNA总长为2 062 bp(包括UTR区),其中开放阅读框(Open reading frame,ORF)714 bp,编码237个氨基酸;虽然在进化树中不属于脊椎动物Rab23进化支,但是AmphiRab23具有保守的Rab23_lke结构域,暗示该基因在进化过程中可能在功能上是保守的.时空表达的研究结果进一步显示,AmphiRab23b基因在胚胎发育中的神经板和消化道中表达,与其脊椎动物同源基因的表达模式相似,这说明该基因可能对文昌鱼神经系统和消化道的发育有重要作用.
Hedgehog신호통로재배태발육과정중발휘착중요작용,동시여다충종류적발생밀절상관.Rab23단백재Hedgehog신호통로중분연착십분중요적각색.목전관우문창어Rab23동원기인적연구부국한우불라리체문창어(Branchiostoma floridae)기인조중적주석.문장수차극륭료중국문창어(Branchiostoma belcheri)Rab23b기인(AmphiRab23b)cDNA전서렬,대기연역적단백서렬진행료서렬비대,진화수분석이급기인시공표체분석.연구결과현시,문창어AmphiRab23b기인적cDNA총장위2 062 bp(포괄UTR구),기중개방열독광(Open reading frame,ORF)714 bp,편마237개안기산;수연재진화수중불속우척추동물Rab23진화지,단시AmphiRab23구유보수적Rab23_lke결구역,암시해기인재진화과정중가능재공능상시보수적.시공표체적연구결과진일보현시,AmphiRab23b기인재배태발육중적신경판화소화도중표체,여기척추동물동원기인적표체모식상사,저설명해기인가능대문창어신경계통화소화도적발육유중요작용.
The hedgehog(Hh)pathway plays an important role dunng the embryonic development and is related to the progression of cancers.Rab23 is a crucial functional molecule in Hh pathway.However,there is no report about amphioxus Rab23 up to now except the annotations of two isoforms in the genome of Florida lancelet(Branchiostoma floridae).Here a 2062 bp full-length eDNA sequence of the Rab23,AmphiRab23b,was isolated from Chinese amphioxus(Branchiostoma belcheri),which included the UTRs and an open reading frame of 714 bp,encoding a protein of 237 amino acids.Phylogenetic analysis suggested that AmphiRab23b failed outside the vertebrate clade.But sequence analysis indicated that this putative AmphiRab23b protein contained a specific Rab23_lke domain,which implied that Rab23 gene was functional conservative during evolution.And its developmental expression pattern showed that AmphiRab23b was expressed in the differentiating neural plate and alimentary canal,as the same as the expression pattern of the homologous vertebrate genes,which suggested that AmphiRab23b may function in the development of nervous system and alimentary canal.