CAJ | 학술논문

从棉花植株根部筛选的108株芽孢杆菌中,通过对棉花黄枯萎主要病原菌大丽轮枝菌拮抗效果的复筛,分离到一株LC-105菌株,其抑菌圈达到20 mm以上;本研究从该菌株的发酵代谢产物中,分离出活性拮抗物质,进一步通过DEAE-Sephadex A-50离子交换层析和Sephacryl-S100凝胶层析对其代谢产物进行了分离纯化,证明该拮抗物质为一种蛋白质,最后通过聚丙烯酰胺凝胶电泳证实了所分离物质的纯度达到电泳纯.
종면화식주근부사선적108주아포간균중,통과대면화황고위주요병원균대려륜지균길항효과적복사,분리도일주LC-105균주,기억균권체도20 mm이상;본연구종해균주적발효대사산물중,분리출활성길항물질,진일보통과DEAE-Sephadex A-50리자교환층석화Sephacryl-S100응효층석대기대사산물진행료분리순화,증명해길항물질위일충단백질,최후통과취병희선알응효전영증실료소분리물질적순도체도전영순.
108 Bacillus strain has been isolated from the roots of cotton ,LC-105 strain has been chosen by the antagonistic effect to the cotton wilt and diameter of the antagonistic circle reach more than 20 mm. Antagonistic material has been separated from the metabolism of the strain. Antagonistic material purifying has been done by DEAE-Sephadex iron-exchange chromatography and Sephacryl-S100 gel chromatography. The antagonistic material is proved to be a kind of protein. Electrophoresis pure has been identified by the polyacrylamide gel electrophoresis method.