CAJ | 학술논문

实验采用木瓜蛋白酶和Alcalase碱性蛋白酶水解鹿茸血,在不同的时间取样,分别测定血管紧张素转化酶(ACE)抑制率、二苯代苦味肼基自由基(DPPH·)清除率和羟自由基(·OH)清除率.结果表明Alcalase蛋白酶水解产物ACE抑制活性和DPPH·清除活性较强,其水解3 h的产物ACE抑制率为90.63%,DPPH·清除率为14.40%;木瓜蛋白酶水解5 h的产物·OH清除率最高,为96.7%.用SPSS10.0软件分析结果,发现ACE抑制率与DPPH·清除率具有显著相关性.
실험채용목과단백매화Alcalase감성단백매수해록용혈,재불동적시간취양,분별측정혈관긴장소전화매(ACE)억제솔、이분대고미정기자유기(DPPH·)청제솔화간자유기(·OH)청제솔.결과표명Alcalase단백매수해산물ACE억제활성화DPPH·청제활성교강,기수해3 h적산물ACE억제솔위90.63%,DPPH·청제솔위14.40%;목과단백매수해5 h적산물·OH청제솔최고,위96.7%.용SPSS10.0연건분석결과,발현ACE억제솔여DPPH·청제솔구유현저상관성.
Red Deer antler blood hydrolysates were prepared by enzymatic hydrolysis with alcalase and papain.The in- hibitory rate of angiotensin Ⅰ-converting enzyme (ACE) and clearance rate of·OH and DPPH·were investigated at different times of hydrolysis.The highest ACE inhibitory rate and DPPH·clearance rate were obtained with alcalase, which were 90.63％ and 14.40％ respectively, and the highest·OH clearance rate was obtained with papain, which was 96.7％.Analyzed by SPSS10.0, the correhtion between ACE inhibitory activity and DPPH·clearance rate was significant at 0.05 level,while there was on significant relationship between ACE and·OH.