中华风湿病学杂志
中華風濕病學雜誌
중화풍습병학잡지
CHINESE JOURNAL OF RHEUMATOLOGY
2011年
1期
26-29
,共4页
石连杰%黄向阳%李敏%叶杨%赵臻怡%钟雪%杨南萍
石連傑%黃嚮暘%李敏%葉楊%趙臻怡%鐘雪%楊南萍
석련걸%황향양%리민%협양%조진이%종설%양남평
红斑狼疮,系统性%聚合酶链反应%干扰素诱导蛋白44%疾病活动积分
紅斑狼瘡,繫統性%聚閤酶鏈反應%榦擾素誘導蛋白44%疾病活動積分
홍반랑창,계통성%취합매련반응%간우소유도단백44%질병활동적분
Lupus erythematosus,systemic%Polymerase chain reaction%Interferon-induced protein 44%Disease activity score
目的 探讨系统性红斑狼疮(SLE)患者外周血白细胞干扰素诱导蛋白 44(IFI44)基因实时定量表达水平与SLE临床表现及病情活动的相关性.方法 收集100例SLE患者,40例非SLE其他自身免疫性疾病患者,40名健康对照人群的临床资料,抽提外周血总RNA并逆转录成eDNA,运用SYBRgreen dve Ⅰ实时定量聚合酶链反应(RT-PCR)检测患者和对照组的IFI44基因定量表达水平,并对其与各临床指标及病情活动度的相关性进行分析.数据分析采用方差分析,采用Pearson及Spearman检验进行相关性分析.结果 ①SLE患者组的IFI44拷贝数26.8±5.3明显高于非SLE组7.4±2.7(P=0.0012)和健康对照组5.2±2.0(P=0.005);而非SLE患者组与健康对照组差异无统计学意义.②SLE重度活动组患者IFI44表达量63.1±22.4明显高于非活动组9.2±1.8(P=0.000)和轻度活动组患者28.0±7.2(P=0.015).③SLE患者组的IFI44拷贝数与SLEDAI积分之间呈正相关(r=0.38,P=0.000),与尿蛋白定量(24 h)也呈正相关(r=0.42,P=0.000).结论 IFI44基因在SLE患者中有明显表达上调现象;检测IFI44的表达水平有助于判断SLE患者病情活动状况.
目的 探討繫統性紅斑狼瘡(SLE)患者外週血白細胞榦擾素誘導蛋白 44(IFI44)基因實時定量錶達水平與SLE臨床錶現及病情活動的相關性.方法 收集100例SLE患者,40例非SLE其他自身免疫性疾病患者,40名健康對照人群的臨床資料,抽提外週血總RNA併逆轉錄成eDNA,運用SYBRgreen dve Ⅰ實時定量聚閤酶鏈反應(RT-PCR)檢測患者和對照組的IFI44基因定量錶達水平,併對其與各臨床指標及病情活動度的相關性進行分析.數據分析採用方差分析,採用Pearson及Spearman檢驗進行相關性分析.結果 ①SLE患者組的IFI44拷貝數26.8±5.3明顯高于非SLE組7.4±2.7(P=0.0012)和健康對照組5.2±2.0(P=0.005);而非SLE患者組與健康對照組差異無統計學意義.②SLE重度活動組患者IFI44錶達量63.1±22.4明顯高于非活動組9.2±1.8(P=0.000)和輕度活動組患者28.0±7.2(P=0.015).③SLE患者組的IFI44拷貝數與SLEDAI積分之間呈正相關(r=0.38,P=0.000),與尿蛋白定量(24 h)也呈正相關(r=0.42,P=0.000).結論 IFI44基因在SLE患者中有明顯錶達上調現象;檢測IFI44的錶達水平有助于判斷SLE患者病情活動狀況.
목적 탐토계통성홍반랑창(SLE)환자외주혈백세포간우소유도단백 44(IFI44)기인실시정량표체수평여SLE림상표현급병정활동적상관성.방법 수집100례SLE환자,40례비SLE기타자신면역성질병환자,40명건강대조인군적림상자료,추제외주혈총RNA병역전록성eDNA,운용SYBRgreen dve Ⅰ실시정량취합매련반응(RT-PCR)검측환자화대조조적IFI44기인정량표체수평,병대기여각림상지표급병정활동도적상관성진행분석.수거분석채용방차분석,채용Pearson급Spearman검험진행상관성분석.결과 ①SLE환자조적IFI44고패수26.8±5.3명현고우비SLE조7.4±2.7(P=0.0012)화건강대조조5.2±2.0(P=0.005);이비SLE환자조여건강대조조차이무통계학의의.②SLE중도활동조환자IFI44표체량63.1±22.4명현고우비활동조9.2±1.8(P=0.000)화경도활동조환자28.0±7.2(P=0.015).③SLE환자조적IFI44고패수여SLEDAI적분지간정정상관(r=0.38,P=0.000),여뇨단백정량(24 h)야정정상관(r=0.42,P=0.000).결론 IFI44기인재SLE환자중유명현표체상조현상;검측IFI44적표체수평유조우판단SLE환자병정활동상황.
Objective To investigate the expression of interferon-induced protein 44 (IFI44) gene in the leukocytes of the peripheral blood samples from patients with systemic lupus erythematosus (SLE), and to evaluate the relationship between the expression level and disease activity. Methods Mononuclear cells in the peripheral blood samples from 100 SLE patients were compared with those of 40 disease controls and 40 healthy donors (HD) and the expression of the IFI44 was evaluated by quantitative real-time PCR.Comparisons between groups were performed with ANOVA, and the correlation analysis between the level of expression was higher in SLE patients than disease controls and healthy donors (26.8±5.3, 7.4±2.7, 5.2±2.0,respectively) (P=0.0012, P=0.005), but no difference was found between disease controls and healthy donors. Mild disease activity and the SLE patients with stable disease (63.1±22.4, 28.0±7.2, 9.2±1.8, respectively)and 24 hours urine protein level (r=0.42, P=0.000). Conclusion IFI44 is demonstrated to be highly expressed in SLE patients. The level of IFI44 may be a promising candidate biomarker for identifying SLE activity.
