CAJ | 학술논문

目的利用磁共振波谱技术探讨颅脑爆震伤后不同时间段脑局部代谢变化.方法新西兰大白兔45只采用随机数字表法分为对照组(10只)和创伤组(35只),采用600 mgTNT当量纸雷管在创伤组兔脑上方约6.5 cm垂直距离爆炸,于伤后1,6,12,24 h、3,7,14 d用磁共振波谱技术观测动物存活情况,并检测脑损伤区病理及磁共振波谱表现,观察乙酰天门冬氨酸(N-acetylaspartate,NAA)/肌酸(creatine,Cr)、胆碱(choline,Cho)/Cr在爆震伤后随时间发展的演变过程.结果创伤组兔存活时间在7 d以上,病理及常规MRI示脑挫伤病灶;NAA/Cr均值在损伤后1 h明显下降,持续至伤后24 h,24 h后义上升,7 d后再次下降.Cho/Cr均值在损伤1 h后即明显升高,12 h后下降,3 d后义逐渐升高.结论磁共振波谱技术可反映兔颅脑爆炸伤不同时间段局部组织的代谢变化,为了解爆雀伤后局部组织变化情况及判断组织损伤类型提供理论依据.
목적 이용자공진파보기술탐토로뇌폭진상후불동시간단뇌국부대사변화.방법 신서란대백토45지채용수궤수자표법분위대조조(10지)화창상조(35지),채용600 mgTNT당량지뢰관재창상조토뇌상방약6.5 cm수직거리폭작,우상후1,6,12,24 h、3,7,14 d용자공진파보기술관측동물존활정황,병검측뇌손상구병리급자공진파보표현,관찰을선천문동안산(N-acetylaspartate,NAA)/기산(creatine,Cr)、담감(choline,Cho)/Cr재폭진상후수시간발전적연변과정.결과 창상조토존활시간재7 d이상,병리급상규MRI시뇌좌상병조;NAA/Cr균치재손상후1 h명현하강,지속지상후24 h,24 h후의상승,7 d후재차하강.Cho/Cr균치재손상1 h후즉명현승고,12 h후하강,3 d후의축점승고.결론 자공진파보기술가반영토로뇌폭작상불동시간단국부조직적대사변화,위료해폭작상후국부조직변화정황급판단조직손상류형제공이론의거.
Objective To evaluate the regional cerebral metabolic changes in different episodes by magnetic resonance spectroscopy (MRS) after explosive brain injury in rabbits. Methods Fortyfive New Zealand white rabbits were randomly divided into eight groups, ie, normal control group( 10 rabbits) and trauma group (35 rabbits). The explosive injury in trauma group was induced by explosion of 600 mg TNT equivalent of paper detonators at 6.5 cm above the rabbit brain. The rabbits in trauma group was divided into 1,6, 12, 24 hours, 3, 7, 14 days subgroups (6 rabbits per group). The survival rate was observed at different time points after explosive injury. The MRS was used to detect the regional cerebral metabolic changes including N-acetylaspartate (NAA)/creatine (Cr) ratio and choline(Cho)/Cr ratio as well as evolution of blast injuries over time. Results The rabbits survived for overseven days in the trauma groups, with typical brain contusion manifested by pathological and conventional MRI. Compared with the normal control group, the NAA/Cr ratio was markedly decreased at one hour after injury, slightly rose again at 24 hours and fell again after seven days. The Cho/Cr ratio was markedly increased at one hour after injury, slightly fell again at 12 hours and rose again at three days after injury.Conclusions MRS can manifest the regional cerebral metabolic changes of rabbits with explosive injury at different time points and hence provide a theoretical basis for understanding the local tissue changes and determining the type of tissue damage after blast injury.