CAJ | 학술논문

目的对新型H1N1从2009年在苏州出现至2011年度基本消失整个过程中,苏州分离株血凝素( haemagglutinin,HA)基因的变化和分子特征进行了分析.方法提取苏州分离株的RNA,以WHO推荐的引物扩增HA基因并测序.采用生物信息学软件对苏州毒株的HA进行详细的分子特征分析,并与疫苗株及全球和全国的代表性毒株进行比对分析.结果与疫苗株相比,5株苏州株的核苷酸序列同源性在98.8％～99.4％之间,氨基酸序列同源性在98.8％～99.4％之间.5株苏州株中3株在1个抗原位点突变,2株有2个抗原位点突变,且有2个抗原位点突变的都是2011年分离株.糖基化位点、二硫键和受体结合位点都没有发生改变.5株苏州株和各地的分离株都表现出按年份聚类的特点.结论详细分析了苏州地区新型H1N1流感病毒HA的分子特征,表明苏州毒株的抗原位点上出现氨基酸残基的变化,但仍处于稳态.
목적 대신형H1N1종2009년재소주출현지2011년도기본소실정개과정중,소주분리주혈응소( haemagglutinin,HA)기인적변화화분자특정진행료분석.방법 제취소주분리주적RNA,이WHO추천적인물확증HA기인병측서.채용생물신식학연건대소주독주적HA진행상세적분자특정분석,병여역묘주급전구화전국적대표성독주진행비대분석.결과 여역묘주상비,5주소주주적핵감산서렬동원성재98.8％～99.4％지간,안기산서렬동원성재98.8％～99.4％지간.5주소주주중3주재1개항원위점돌변,2주유2개항원위점돌변,차유2개항원위점돌변적도시2011년분리주.당기화위점、이류건화수체결합위점도몰유발생개변.5주소주주화각지적분리주도표현출안년빈취류적특점.결론 상세분석료소주지구신형H1N1류감병독HA적분자특정,표명소주독주적항원위점상출현안기산잔기적변화,단잉처우은태.
Objective To investigate the genetic variation and molecular characteristics of HA gene of influenza A (H1N1) virus isolated in Suzhou during from2009 to 2011.Methods Viral RNA of 5 Suzhou isolates was isolated and their HA gene were amplified and sequenced by the primers and protocol recommended by WHO,and the sequences together with other sequences downloaded from GenBank were analyzed by several bioinformatics software.Results Compared with vaccine strain,the average homogeneity of nucleotide and amino acids of 5 Suzhou isolates were between 98.8-99.4％ and 98.8-99.4％ respectively.All of the 5 strains have 1 amino acids replacement in Sb region and 2 strains have 2 amino acids replacement in Ca region.Strains from and outside Suzhou both showed a trend of clustering by collection year.Conclusion The Suzhou strains are still in stable condition although 1-2 amino acids replacement had happened in antigenic sites.