中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2009年
8期
986-987
,共2页
陶卫平%冯觉平%李敏%王亚萍%谢涛%鲍文菁
陶衛平%馮覺平%李敏%王亞萍%謝濤%鮑文菁
도위평%풍각평%리민%왕아평%사도%포문정
癌,肝细胞%CD44%Ezrin%三氧化二砷
癌,肝細胞%CD44%Ezrin%三氧化二砷
암,간세포%CD44%Ezrin%삼양화이신
Carcinoma,hepatocellular%CD44%Ezrin%Arsenic trioxide
目的 观察肝癌HepG2细胞转移相关基因CD44v6、Ezrin mRNA表达及三氧化二砷(As2O3)对其表达的影响.方法 以逆转录一聚合酶链反应(RT-PCR)方法检测HepG2细胞中CIM4v6、Ezrin基因mRNA的表达及As2O3作用后其表达水平的变化.结果 HepG2细胞CD44v6及Ezrin mRNA均有表达.CD44v6-mRNA表达水平为1.57±0.47;经0.25 mg/L As2O3作用4、6、8 d后其表达水平分别下降到0.95±0.25、0.90±0.24和0.63±0.19(P<0.05),下降水平呈时间依赖;Ezrin-mRNA表达水平为0.70±0.18,加入0.25 mg/L的As2O3 4、6、8 d后,表达水平分别为0.70±0.21、0.75±0.22和0.71±0.20,各时间段Ezrin-mRNA的表达水平无明显变化(P>0.05).结论 As2O3可显著下调HepG2细胞CD44v6-mRNA的表达,对Ezrin-mRNA的表达可能无直接调节作用.
目的 觀察肝癌HepG2細胞轉移相關基因CD44v6、Ezrin mRNA錶達及三氧化二砷(As2O3)對其錶達的影響.方法 以逆轉錄一聚閤酶鏈反應(RT-PCR)方法檢測HepG2細胞中CIM4v6、Ezrin基因mRNA的錶達及As2O3作用後其錶達水平的變化.結果 HepG2細胞CD44v6及Ezrin mRNA均有錶達.CD44v6-mRNA錶達水平為1.57±0.47;經0.25 mg/L As2O3作用4、6、8 d後其錶達水平分彆下降到0.95±0.25、0.90±0.24和0.63±0.19(P<0.05),下降水平呈時間依賴;Ezrin-mRNA錶達水平為0.70±0.18,加入0.25 mg/L的As2O3 4、6、8 d後,錶達水平分彆為0.70±0.21、0.75±0.22和0.71±0.20,各時間段Ezrin-mRNA的錶達水平無明顯變化(P>0.05).結論 As2O3可顯著下調HepG2細胞CD44v6-mRNA的錶達,對Ezrin-mRNA的錶達可能無直接調節作用.
목적 관찰간암HepG2세포전이상관기인CD44v6、Ezrin mRNA표체급삼양화이신(As2O3)대기표체적영향.방법 이역전록일취합매련반응(RT-PCR)방법검측HepG2세포중CIM4v6、Ezrin기인mRNA적표체급As2O3작용후기표체수평적변화.결과 HepG2세포CD44v6급Ezrin mRNA균유표체.CD44v6-mRNA표체수평위1.57±0.47;경0.25 mg/L As2O3작용4、6、8 d후기표체수평분별하강도0.95±0.25、0.90±0.24화0.63±0.19(P<0.05),하강수평정시간의뢰;Ezrin-mRNA표체수평위0.70±0.18,가입0.25 mg/L적As2O3 4、6、8 d후,표체수평분별위0.70±0.21、0.75±0.22화0.71±0.20,각시간단Ezrin-mRNA적표체수평무명현변화(P>0.05).결론 As2O3가현저하조HepG2세포CD44v6-mRNA적표체,대Ezrin-mRNA적표체가능무직접조절작용.
Objective To investigate the expression of CD44v6,and Ezrin mRNA in human hepatocellular carcinoma cells (HepG2), and evaluate the effects of arsenic trioxide (As2O3) on the expression in HepG2 in vitro.Methods The expression levels of CD44v6 and Ezrin mRNA were examined by RT-PCR in HepG2 cells in vitro.Results The expression of CD44v6 and Ezrin mRNA was detected in HepG2 cells.The expression levels of CD44v6 and Ezrin mRNA were 1.57 ± 0.47 and 0.70 ±0.18, respectively.A significant down-regulation of expression of CD44v6 mRNA was observed in HepG2 cells when 0.25 mg/L As2O3 was given at the 4th,6th,and 8th day (0.95±0.25,0.90±0.24,and 0.63± 0.19,P <0.05).No change was found in the expression of Ezfin mRNA when HepG2 cells were treated with As2O3 for 4,6, or 8 days (0.70 ± 0.21,0.75 ± 0.22, and 0.71 ± 0.20, P 0.05).Conclusion As2O3 can decrease the expression of CD44v6 mRNA, but it can not directly regulate the expression of Ezrin mRNA in HepG2 cells in vitro.
