中华检验医学杂志
中華檢驗醫學雜誌
중화검험의학잡지
CHINESE JOURNAL OF LABORATORY MEDICINE
2009年
7期
772-776
,共5页
熊怡淞%周运恒%吴炜霖%张玲珍%梁艳%杨再兴%耿红莲%王皓%王爱华%荣光华%仲人前
熊怡淞%週運恆%吳煒霖%張玲珍%樑豔%楊再興%耿紅蓮%王皓%王愛華%榮光華%仲人前
웅이송%주운항%오위림%장령진%량염%양재흥%경홍련%왕호%왕애화%영광화%중인전
冠心病%白细胞,单核%膜糖蛋白类%受体,免疫%流式细胞术
冠心病%白細胞,單覈%膜糖蛋白類%受體,免疫%流式細胞術
관심병%백세포,단핵%막당단백류%수체,면역%류식세포술
Coronary disease%Leukocytes,mononuclear%Membrane glycoproteins%Receptors,immunologic%Flow cytometry
目的 观察唾液酸结合免疫球蛋白样凝集素1(sialic acid-binding immunoglobulin-likelectin 1,Siglec-1,也称CD169)在冠状动脉粥样硬化性心脏病(CHD)患者外周血淋巴细胞、单核细胞和中性粒细胞上的表达水平,并探讨其与冠状动脉粥样硬化发生发展的关系.方法 流式细胞术检测57例CHD患者及38名健康对照者外周血CD14CD169双阳性细胞的表达率;生化常规测定所有入选者血脂水平;实时荧光相对定量逆转录(FQ-RT)-PCR方法检测入选对象外周血单个核细胞(PBMCs)中CD169 mRNA的含量.结果 流式细胞仪检测发现,CD169在健康对照组及CHD组淋巴细胞和中性粒细胞上均无表达;CHD组单核细胞CD14CD169双阳性率为(12.7±2.4)%,显著高于健康对照组[(1.0±0.3)%,t=23.2,P<0.01];CHD组CD169 mRNA的拷贝数显著高于健康对照组拷贝数(t=6.59,P<0.01),为健康对照组的3..倍;而CHD血脂正常组和血脂异常组的CD169阳性率[分别为(12.2±2.3)%和(13.4±2.5)%,t=1.87,P>0.05]和mRNA平均拷贝数(分别为健康对照组的3.64和2.79倍,t=0.98,P>0.05)差异均无统计学意义.结论 CD169组成性表达于特定组织巨噬细胞上,健康人外周血单核细胞上并不表达,单核巨噬细胞受到炎症刺激时,CD169表达迅速上调.CD169蛋白及mRNA含量在CHD患者外周血单核细胞上表达显著升高,CHD患者外周血单核细胞已发生巨噬细胞化,单核巨噬细胞介导的免疫炎症反应在CHD发生发展过程中起重要作用.
目的 觀察唾液痠結閤免疫毬蛋白樣凝集素1(sialic acid-binding immunoglobulin-likelectin 1,Siglec-1,也稱CD169)在冠狀動脈粥樣硬化性心髒病(CHD)患者外週血淋巴細胞、單覈細胞和中性粒細胞上的錶達水平,併探討其與冠狀動脈粥樣硬化髮生髮展的關繫.方法 流式細胞術檢測57例CHD患者及38名健康對照者外週血CD14CD169雙暘性細胞的錶達率;生化常規測定所有入選者血脂水平;實時熒光相對定量逆轉錄(FQ-RT)-PCR方法檢測入選對象外週血單箇覈細胞(PBMCs)中CD169 mRNA的含量.結果 流式細胞儀檢測髮現,CD169在健康對照組及CHD組淋巴細胞和中性粒細胞上均無錶達;CHD組單覈細胞CD14CD169雙暘性率為(12.7±2.4)%,顯著高于健康對照組[(1.0±0.3)%,t=23.2,P<0.01];CHD組CD169 mRNA的拷貝數顯著高于健康對照組拷貝數(t=6.59,P<0.01),為健康對照組的3..倍;而CHD血脂正常組和血脂異常組的CD169暘性率[分彆為(12.2±2.3)%和(13.4±2.5)%,t=1.87,P>0.05]和mRNA平均拷貝數(分彆為健康對照組的3.64和2.79倍,t=0.98,P>0.05)差異均無統計學意義.結論 CD169組成性錶達于特定組織巨噬細胞上,健康人外週血單覈細胞上併不錶達,單覈巨噬細胞受到炎癥刺激時,CD169錶達迅速上調.CD169蛋白及mRNA含量在CHD患者外週血單覈細胞上錶達顯著升高,CHD患者外週血單覈細胞已髮生巨噬細胞化,單覈巨噬細胞介導的免疫炎癥反應在CHD髮生髮展過程中起重要作用.
목적 관찰타액산결합면역구단백양응집소1(sialic acid-binding immunoglobulin-likelectin 1,Siglec-1,야칭CD169)재관상동맥죽양경화성심장병(CHD)환자외주혈림파세포、단핵세포화중성립세포상적표체수평,병탐토기여관상동맥죽양경화발생발전적관계.방법 류식세포술검측57례CHD환자급38명건강대조자외주혈CD14CD169쌍양성세포적표체솔;생화상규측정소유입선자혈지수평;실시형광상대정량역전록(FQ-RT)-PCR방법검측입선대상외주혈단개핵세포(PBMCs)중CD169 mRNA적함량.결과 류식세포의검측발현,CD169재건강대조조급CHD조림파세포화중성립세포상균무표체;CHD조단핵세포CD14CD169쌍양성솔위(12.7±2.4)%,현저고우건강대조조[(1.0±0.3)%,t=23.2,P<0.01];CHD조CD169 mRNA적고패수현저고우건강대조조고패수(t=6.59,P<0.01),위건강대조조적3..배;이CHD혈지정상조화혈지이상조적CD169양성솔[분별위(12.2±2.3)%화(13.4±2.5)%,t=1.87,P>0.05]화mRNA평균고패수(분별위건강대조조적3.64화2.79배,t=0.98,P>0.05)차이균무통계학의의.결론 CD169조성성표체우특정조직거서세포상,건강인외주혈단핵세포상병불표체,단핵거서세포수도염증자격시,CD169표체신속상조.CD169단백급mRNA함량재CHD환자외주혈단핵세포상표체현저승고,CHD환자외주혈단핵세포이발생거서세포화,단핵거서세포개도적면역염증반응재CHD발생발전과정중기중요작용.
Objective To investigate the expression of sialic acid-binding immunoglobulin-like lectin-one (Siglec-1, also called CD169) in lymphocytes, monocytes and neutrophils in peripheral blood in patients with coronary heart disease(CHD), and explore the relationship between Siglec-1 expression and atheresclerosis. Methods CD145 CD169 positive cell proportion and CD169 mRNA levels were respectively measured by flow cytometry and real-time quantitative reverse transcription-polymerase chain reaction (FQ-RT-PCR) in 57 CHD patients and 38 healthy controls. And the levels of serum hpids were determined by automatic biochemistry analyzer. Results The flow cytometry analysis showed that CD169 protein was not found in lymphocytes and neutrophils in both CHD patients and healthy controls. The rate of CD14 CD169 double positive ceils in monocytes in CHD group was significandy higher than that in healthy controls [(12.7±2.4)% vs (1.0±0.3)% ,t =23.2,P<0.01]. And FQ-RT-PCR analysis showed that the mean CD± mRNA copy number in PBMCs in CHD group was significantly higher(3.2 fold) than that in healthy controls [t = 6. 59, P < 0.01]. However, neither differences of CD169 protein positivities [[(12. 2 ± 2. 3) %vs (13.4±2.5)% ,t = 1.87,P >0.05] nor mRNA levels [3.64 fold vs 2.79 fold when compared with healthy controls,t =0. 98, P > 0. 05] were found between CHD patients with normal and abnormal levels of serum Lipids. Conclusions CD169 is mainly expressed in human tissue-resident macrophages but not expressed in peripheral blood monecytes. And when the monocytes is stimulated by inflammation, the expression of CD169 is increased. In patients with CHD, the increased expression of CD169 protein and mRNA level has demonstrated the activation of monocytes in peripheral blood. CD169 and CD169-mediated monocytes activation may play an important role in the development and progression of atherosclerosis.
