CAJ | 학술논문

于12月取材的软枣猕猴桃侧芽通过酒精和次氯酸钠灭菌以后,以此为试材探讨了冻结保存液的组成、温度和浸泡时间对其冻结保存的影响.结果表明:最适的DMSO的浓度为15%,最适的浸泡温度为0℃.在侧芽预冷到-30℃后,再投入到液氮中,解冻后的生存率在含有甘油8 mol/L和11.5 mol/L区最高.用甘油预处理的实验结果表明:在处理浓度较高时可以获得较高的生存率.然而,在甘油预浸后,再用冻结保存液处理的最佳时间为小于1 h.
우12월취재적연조미후도측아통과주정화차록산납멸균이후,이차위시재탐토료동결보존액적조성、온도화침포시간대기동결보존적영향.결과표명:최괄적DMSO적농도위15%,최괄적침포온도위0℃.재측아예랭도-30℃후,재투입도액담중,해동후적생존솔재함유감유8 mol/L화11.5 mol/L구최고.용감유예처리적실험결과표명:재처리농도교고시가이획득교고적생존솔.연이,재감유예침후,재용동결보존액처리적최가시간위소우1 h.
Lateral buds of tara vine (A ctinidia arguta Planch.) were collected in late December and surface-sterilized with ethanol, followed by sodium hypochlorite solution. Effects of the situation of freezing solution in different composition, temperature and immersing duration were investigated. The optimum concentration of DMSO for cryopreservation of the buds was 15%, and the optimum temperature of freezing solution was 0℃. The buds immersed in liquid nitrogen after pre-freezing of freezing to -30℃ showed the highest survival rates at high concentrations (8 mol/L and 11.5 mol/L) of glycerol. The buds loaded with glycerol before immersing in freezing solution gave the highest survival rate with a loading solution containing higher concentrations of glycerol. The highest survival rates were obtained with lateral buds immersed in the freezing solution for 0, 0.5 and 1 hours.