背景:脑卒中易感型自发性高血压大鼠(SHRsp)是常用的高血压脑卒中动物模型.因其高血压使保种有一定难度,易受环境情况影响,从而使脑卒中特性易发生变异.目的:观察SHRsp的繁育能力和高血压脑卒中特性的保持.设计:以大鼠为观察对象,观察对比实验.单位:中国医学科学院阜外医院心血管病研究所.材料:实验于1999-09/2001-12在中国医学科学院阜外医院心血管病研究所进行.选择SHRsp种鼠93对186只,8周龄SHRsp大鼠48只和正常血压大鼠(WKY)98对196只进入实验.方法:采用兄妹交配方法,观察记录SHRsp 2年的受孕率、孕鼠产仔数(仅计算未吃仔母鼠的产仔数),母鼠吃仔率,并与同期WKY大鼠比较;分别于12,16,20周龄时测量种鼠的收缩压和心率.另取48只8周龄SHRsp在10 g/L盐水负荷下加速脑卒中的发生,自然死亡或盐负荷12周后断头处死,取大脑组织通过苏木精-伊红染色等处理后,在光镜下进行脑组织病理观察,检测脑卒中发生率.主要观察指标:2年雌鼠受孕率,孕鼠产仔率,母鼠吃仔率.种鼠收缩压,心率,脑卒中病理检出率.结果:SHRsp种鼠93对186只,WKY 98对196只,48只8周龄SHRsp均进入结果分析.第1年度生产2代,平均SHRsp受孕率,孕鼠平均产仔数(100%,10.3只)高于同期WKY(90%,P=0.077;6.5只,P＜0.001).第2年度生产2代,平均SHRsp受孕率,孕鼠平均产仔数(89%,8.2只)高于同期WKY(59%,P＜0.001;4.3只,P＜0.001).2年内SHRsp母鼠受孕87只,WKY母鼠受孕67只,SHRsp母鼠产仔后4周内吃仔率为6%(5/87),低于WKY吃仔率(18%,12/67),P＞0.05.12周龄SHRsp雄鼠收缩压191.6～223.8 mm Hg(1 mm Hg=0.133 kPa),雌鼠为174.2～196.3mmHg,16周龄雄鼠收缩压为219.0～224.9mmHg,20周龄雄鼠收缩压达232.0～242.6 mm Hg.SHRsp血压随周龄增加而升高.12周龄SHRsp雄鼠心率388～428次/min,雌鼠为373～417次/min.16周龄雄鼠心率392～410次/min,20周龄心率404～425次/min.48只雄鼠SHRsp脑卒中病理检出率为81%(39/48).结论:SHRsp繁育能力与WKY相似.以血压、心率和脑组织病理检查保持了SHRsp高血压和脑卒中的特性,可做为高血压脑卒中的实验模型.
揹景:腦卒中易感型自髮性高血壓大鼠(SHRsp)是常用的高血壓腦卒中動物模型.因其高血壓使保種有一定難度,易受環境情況影響,從而使腦卒中特性易髮生變異.目的:觀察SHRsp的繁育能力和高血壓腦卒中特性的保持.設計:以大鼠為觀察對象,觀察對比實驗.單位:中國醫學科學院阜外醫院心血管病研究所.材料:實驗于1999-09/2001-12在中國醫學科學院阜外醫院心血管病研究所進行.選擇SHRsp種鼠93對186隻,8週齡SHRsp大鼠48隻和正常血壓大鼠(WKY)98對196隻進入實驗.方法:採用兄妹交配方法,觀察記錄SHRsp 2年的受孕率、孕鼠產仔數(僅計算未喫仔母鼠的產仔數),母鼠喫仔率,併與同期WKY大鼠比較;分彆于12,16,20週齡時測量種鼠的收縮壓和心率.另取48隻8週齡SHRsp在10 g/L鹽水負荷下加速腦卒中的髮生,自然死亡或鹽負荷12週後斷頭處死,取大腦組織通過囌木精-伊紅染色等處理後,在光鏡下進行腦組織病理觀察,檢測腦卒中髮生率.主要觀察指標:2年雌鼠受孕率,孕鼠產仔率,母鼠喫仔率.種鼠收縮壓,心率,腦卒中病理檢齣率.結果:SHRsp種鼠93對186隻,WKY 98對196隻,48隻8週齡SHRsp均進入結果分析.第1年度生產2代,平均SHRsp受孕率,孕鼠平均產仔數(100%,10.3隻)高于同期WKY(90%,P=0.077;6.5隻,P＜0.001).第2年度生產2代,平均SHRsp受孕率,孕鼠平均產仔數(89%,8.2隻)高于同期WKY(59%,P＜0.001;4.3隻,P＜0.001).2年內SHRsp母鼠受孕87隻,WKY母鼠受孕67隻,SHRsp母鼠產仔後4週內喫仔率為6%(5/87),低于WKY喫仔率(18%,12/67),P＞0.05.12週齡SHRsp雄鼠收縮壓191.6～223.8 mm Hg(1 mm Hg=0.133 kPa),雌鼠為174.2～196.3mmHg,16週齡雄鼠收縮壓為219.0～224.9mmHg,20週齡雄鼠收縮壓達232.0～242.6 mm Hg.SHRsp血壓隨週齡增加而升高.12週齡SHRsp雄鼠心率388～428次/min,雌鼠為373～417次/min.16週齡雄鼠心率392～410次/min,20週齡心率404～425次/min.48隻雄鼠SHRsp腦卒中病理檢齣率為81%(39/48).結論:SHRsp繁育能力與WKY相似.以血壓、心率和腦組織病理檢查保持瞭SHRsp高血壓和腦卒中的特性,可做為高血壓腦卒中的實驗模型.
배경:뇌졸중역감형자발성고혈압대서(SHRsp)시상용적고혈압뇌졸중동물모형.인기고혈압사보충유일정난도,역수배경정황영향,종이사뇌졸중특성역발생변이.목적:관찰SHRsp적번육능력화고혈압뇌졸중특성적보지.설계:이대서위관찰대상,관찰대비실험.단위:중국의학과학원부외의원심혈관병연구소.재료:실험우1999-09/2001-12재중국의학과학원부외의원심혈관병연구소진행.선택SHRsp충서93대186지,8주령SHRsp대서48지화정상혈압대서(WKY)98대196지진입실험.방법:채용형매교배방법,관찰기록SHRsp 2년적수잉솔、잉서산자수(부계산미흘자모서적산자수),모서흘자솔,병여동기WKY대서비교;분별우12,16,20주령시측량충서적수축압화심솔.령취48지8주령SHRsp재10 g/L염수부하하가속뇌졸중적발생,자연사망혹염부하12주후단두처사,취대뇌조직통과소목정-이홍염색등처리후,재광경하진행뇌조직병리관찰,검측뇌졸중발생솔.주요관찰지표:2년자서수잉솔,잉서산자솔,모서흘자솔.충서수축압,심솔,뇌졸중병리검출솔.결과:SHRsp충서93대186지,WKY 98대196지,48지8주령SHRsp균진입결과분석.제1년도생산2대,평균SHRsp수잉솔,잉서평균산자수(100%,10.3지)고우동기WKY(90%,P=0.077;6.5지,P＜0.001).제2년도생산2대,평균SHRsp수잉솔,잉서평균산자수(89%,8.2지)고우동기WKY(59%,P＜0.001;4.3지,P＜0.001).2년내SHRsp모서수잉87지,WKY모서수잉67지,SHRsp모서산자후4주내흘자솔위6%(5/87),저우WKY흘자솔(18%,12/67),P＞0.05.12주령SHRsp웅서수축압191.6～223.8 mm Hg(1 mm Hg=0.133 kPa),자서위174.2～196.3mmHg,16주령웅서수축압위219.0～224.9mmHg,20주령웅서수축압체232.0～242.6 mm Hg.SHRsp혈압수주령증가이승고.12주령SHRsp웅서심솔388～428차/min,자서위373～417차/min.16주령웅서심솔392～410차/min,20주령심솔404～425차/min.48지웅서SHRsp뇌졸중병리검출솔위81%(39/48).결론:SHRsp번육능력여WKY상사.이혈압、심솔화뇌조직병리검사보지료SHRsp고혈압화뇌졸중적특성,가주위고혈압뇌졸중적실험모형.
BACKGROUND: Stroke-prone spontaneously hypertensive rat(SHRsp) is an animal model of hypertensive stroke commonly used. The species is hard to be maintained due to hypertension and easily affected by environmental factors, therefore the characteristics of stroke are always aberrated.OBJECTIVE: To observe the abilities of reproduction and maintaining the characteristics of hypertensive stroke in SHPsp.DESIGN: Observational controlled study based on rats.SETTING: Cardiovascular Disease Institute and Fuwai Hospital of Chinese Academy of Medical Sciences.MATERIALS: The experiment was conducted Cardiovascular Disease Institute and Fuwai Hospital of Chinese Academy of Medical Sciences during September 1999 to December 2001. Totally 93 pairs(186) strain SHRsp, 48stroke-prone spontaneously hypertensive rats of 8 weeks age as well as 98pairs(196) rats of normal blood pressure [Wistar Kyoto rat (WKY) ] were selected.METHODS: Brother sister mating method was used. And the conception rate, number of litter size(only calculating the litter size of mother rat not eating neonatal rats), rate of eating neonatal rats by mother rats were recorded and compared with those of WKY. The systolic pressure and heart rate of strain rat were measured when they were 12, 16 and 20 weeks old. In addition, 488-week SHRsp were loaded 10 g/L salty water to accelerate the occurrence of stroke and hypertension and executed when they naturally dead or 12 weeks after salty water load. The brain tissue was processed by H-E staining and observed under microscope to detect the incidence of stroke.MAIN OUTCOME MEASURES: Conception rate of female rats within 2years; litter rate of pregnant rats; eating, rate of eating neonatal rats by mother rats; systolic pressure of strain rat; heart rate; detection rate of stroke.RESULTS: Totally 93 pairs(186) strain rats of SHRsp, 98 pairs(196) WKY and 48 SHRsp of 8 weeks old entered the final analysis. In the first year there were 2 generations delivered, the average conception rate( 100% ) and average litter number (10.3 rats) of SHRsp were higher than those of WKY of the same term(90%, P ＜ 0. 001; 6. 5 rats, P ＜ 0. 001) . In the second year, there were 2 generations of which the conception rate and average litter number(89%, 8.2 rats) of SHRsp were higher than those of WKY(59%, P ＜ 0. 001; 4. 3 rats, P ＜ 0. 001). There were 87 SHRsp female rats and 67normal blood pressure rats pregnant within 2 years, the rate of eating neonatal rats within 4 weeks postnatal was 6% (5/87) which was lower than that of WKY(18%, 12/67), P ＞ 0.05. The systolic pressure of 12weeks old male rats and female rats was 191.6-223.8 mm Hg and 174. 2-196. 3 mm Hg respectively, while that of 16-week old and 20-week male rats were 219.0 -224. 9 mm Hg and 232.0 -242.6 mm Hg respectively. The blood pressure of SHRsp increased with the advancing of age. The heart rate of 12-week old male and female rats was 388-428 times per minute and 373-417times/minute respectively while that of 16-week and 20-week male rats were 392 -410 times per minute and 404-425 times per minute respectively. The pathological detection rate of 48 SHRsp was 81% (39/48).CONCLUSION: The reproduction ability of SHRsp is similar to normal rats. The blood pressure, heart rate and pathological examination of brain tissue of them all maintain the characteristics of hypertension and stroke so that they can be used as qualified experimental model.
