CAJ | 학술논문

目的探讨激活的NF-κB及调节活化正常T细胞表达和趋化因子(RANTES)的表达及其在升主动脉瘤形成中的作用.方法 4周龄雌性Wistar大鼠40只,随机分成升主动脉瘤组(20只)和正常对照组(20只).制作升主动脉瘤动物模型,术后3个月取材,免疫组织化学方法分析NF-κB、RANTES的蛋白表达,RT-PCR方法检测其mRNA的表达.结果模型组动脉壁NF-κB和RANTES表达明显升高,而正常动脉壁中未见或极少见NF-κB和RANTES表达.RT-PCR显示,模型组的动脉瘤内NF-κB和RANTES mRNA表达增强,两者呈显著性正相关.结论 NF-κB和RANTES在动脉瘤中表达强于对照组,NF-κB和RANTES在升主动脉瘤病变的发生发展过程中可能起重要作用.
목적 탐토격활적NF-κB급조절활화정상T세포표체화추화인자(RANTES)적표체급기재승주동맥류형성중적작용.방법 4주령자성Wistar대서40지,수궤분성승주동맥류조(20지)화정상대조조(20지).제작승주동맥류동물모형,술후3개월취재,면역조직화학방법분석NF-κB、RANTES적단백표체,RT-PCR방법검측기mRNA적표체.결과 모형조동맥벽NF-κB화RANTES표체명현승고,이정상동맥벽중미견혹겁소견NF-κB화RANTES표체.RT-PCR현시,모형조적동맥류내NF-κB화RANTES mRNA표체증강,량자정현저성정상관.결론 NF-κB화RANTES재동맥류중표체강우대조조,NF-κB화RANTES재승주동맥류병변적발생발전과정중가능기중요작용.
Objective To investigate the expression of nuclear factor kappaB(NF-κB) and regulated upon activation normal T cell expressed and secreted chemokine(RANTES) during the formatiom of ascending aortic aneurysm. Methods Forty Wistar rats were randomly divided into the control group(n=20) and the experimental group(n=20).The rat models were made by ligating the ascending aorta. The ascending aortas were taken after ligation for 3months. Immunohistochemistry staining was performed to detect the protein expression of NF-κB and RANTES. The expression of NF-κB and RANTES mRNA were detected by RT-PCR. Results Immunohistochemisry staining results showed NF-κB and RANTES expression significantly increased in aneurysm, while there was a little positive staining in the control group. RT-PCR results indicated that the expression levels of NF-κB and RANTES in the aneurysm were stonger than that of the control group. The expression of NF-κB and RANTES mRNA were remarkably correlated. Conclusion The expression of NF-κB and RANTES in ascendin aortic aneurysm are stronger than that in the control. NF-κB and RANTES may contribute to the pathogenesis of the ascending aortic aneurysm.