中国组织工程研究与临床康复
中國組織工程研究與臨床康複
중국조직공정연구여림상강복
JOURNAL OF CLINICAL REHABILITATIVE TISSUE ENGINEERING RESEARCH
2009年
47期
9305-9308
,共4页
彭亦龙%杨秋会%谭绍早%郑文杰%薛琰%鲁丹萍%徐迎宾
彭亦龍%楊鞦會%譚紹早%鄭文傑%薛琰%魯丹萍%徐迎賓
팽역룡%양추회%담소조%정문걸%설염%로단평%서영빈
季铵化磷酸锆%制备%组成结构%抗菌活性
季銨化燐痠鋯%製備%組成結構%抗菌活性
계안화린산고%제비%조성결구%항균활성
背景:目前已经对金属离子化磷酸锆的制备及抗菌活性进行了系统研究,但易变色或抗菌活性不足的缺点限制了它们的广泛应用.课题组拟将高效、广谱、低毒季铵盐杀菌剂通过离子交换法负载在磷酸锆钠上希望能够制备出颜色稳定、抗菌活性高的新型季铵化磷酸锆抗菌剂.目的:分析季铵化磷酸锆的组成结构和抗菌活性.设计、时间及地点:体外观察实验,于2009-06/08在暨南大学化学系科研实验室完成.材料:采用离子交换法将十二烷基二甲基苄基氯化铵引入磷酸锆钠中制备季铵化磷酸锆.方法:反应体系中按季铵阳离子与磷酸锆钠的阳离子交换容量的摩尔比分别为0.25:1,0.5:1,1.0:1和1.5:1加料,采用离子交换法制备了4种不同季铵阳离子含量的季铵化磷酸锆(QZrP-1,QZrP-2,QZrP-3,QZrP-4).主要观察指标:分别通过红外光谱仪,元素分析仪,热分析仪分析样品的组成结构和热稳定性.采用试管稀释法测定样品对大肠杆菌和金黄色葡萄球菌的最低抑菌浓度和最低杀菌浓度.结果:季铵化磷酸锆中结合了季铵阳离子,其含量随着反应液中季铵盐添加量的增加而增加.季铵化磷酸锆QZrP-1、QZrP-2、QZrP-3、QZrP-4中季铵阳离子的质量分数分别为3.70%、5.00%、6.96%和10.01%.季铵化磷酸锆中季铵阳离子的热分解起始温度都大于345℃,具有良好的热稳定性.随着季铵阳离子含量的增加,季铵化磷酸锆的抗菌活性增强.所制备的季铵阳离子质量分数为6.96%的QZrP-3样品,其对E.coil、S.aureus都显示出良好的抗菌活性.结论:季铵阳离子质量分数为6.96%的季铵化磷酸锆(QZrP-3)热稳定性好、抗菌活性高.
揹景:目前已經對金屬離子化燐痠鋯的製備及抗菌活性進行瞭繫統研究,但易變色或抗菌活性不足的缺點限製瞭它們的廣汎應用.課題組擬將高效、廣譜、低毒季銨鹽殺菌劑通過離子交換法負載在燐痠鋯鈉上希望能夠製備齣顏色穩定、抗菌活性高的新型季銨化燐痠鋯抗菌劑.目的:分析季銨化燐痠鋯的組成結構和抗菌活性.設計、時間及地點:體外觀察實驗,于2009-06/08在暨南大學化學繫科研實驗室完成.材料:採用離子交換法將十二烷基二甲基芐基氯化銨引入燐痠鋯鈉中製備季銨化燐痠鋯.方法:反應體繫中按季銨暘離子與燐痠鋯鈉的暘離子交換容量的摩爾比分彆為0.25:1,0.5:1,1.0:1和1.5:1加料,採用離子交換法製備瞭4種不同季銨暘離子含量的季銨化燐痠鋯(QZrP-1,QZrP-2,QZrP-3,QZrP-4).主要觀察指標:分彆通過紅外光譜儀,元素分析儀,熱分析儀分析樣品的組成結構和熱穩定性.採用試管稀釋法測定樣品對大腸桿菌和金黃色葡萄毬菌的最低抑菌濃度和最低殺菌濃度.結果:季銨化燐痠鋯中結閤瞭季銨暘離子,其含量隨著反應液中季銨鹽添加量的增加而增加.季銨化燐痠鋯QZrP-1、QZrP-2、QZrP-3、QZrP-4中季銨暘離子的質量分數分彆為3.70%、5.00%、6.96%和10.01%.季銨化燐痠鋯中季銨暘離子的熱分解起始溫度都大于345℃,具有良好的熱穩定性.隨著季銨暘離子含量的增加,季銨化燐痠鋯的抗菌活性增彊.所製備的季銨暘離子質量分數為6.96%的QZrP-3樣品,其對E.coil、S.aureus都顯示齣良好的抗菌活性.結論:季銨暘離子質量分數為6.96%的季銨化燐痠鋯(QZrP-3)熱穩定性好、抗菌活性高.
배경:목전이경대금속리자화린산고적제비급항균활성진행료계통연구,단역변색혹항균활성불족적결점한제료타문적엄범응용.과제조의장고효、엄보、저독계안염살균제통과리자교환법부재재린산고납상희망능구제비출안색은정、항균활성고적신형계안화린산고항균제.목적:분석계안화린산고적조성결구화항균활성.설계、시간급지점:체외관찰실험,우2009-06/08재기남대학화학계과연실험실완성.재료:채용리자교환법장십이완기이갑기변기록화안인입린산고납중제비계안화린산고.방법:반응체계중안계안양리자여린산고납적양리자교환용량적마이비분별위0.25:1,0.5:1,1.0:1화1.5:1가료,채용리자교환법제비료4충불동계안양리자함량적계안화린산고(QZrP-1,QZrP-2,QZrP-3,QZrP-4).주요관찰지표:분별통과홍외광보의,원소분석의,열분석의분석양품적조성결구화열은정성.채용시관희석법측정양품대대장간균화금황색포도구균적최저억균농도화최저살균농도.결과:계안화린산고중결합료계안양리자,기함량수착반응액중계안염첨가량적증가이증가.계안화린산고QZrP-1、QZrP-2、QZrP-3、QZrP-4중계안양리자적질량분수분별위3.70%、5.00%、6.96%화10.01%.계안화린산고중계안양리자적열분해기시온도도대우345℃,구유량호적열은정성.수착계안양리자함량적증가,계안화린산고적항균활성증강.소제비적계안양리자질량분수위6.96%적QZrP-3양품,기대E.coil、S.aureus도현시출량호적항균활성.결론:계안양리자질량분수위6.96%적계안화린산고(QZrP-3)열은정성호、항균활성고.
BACKGROUND: The preparation and antibacterial activity of metal ionic zirconium phosphates has been systemically investigated now, but the applications are limited owing to the discoloration or the low antibacterial activity. Here we prepared new antibacterial agents of quaternized zirconium phosphates by introducing quaternary ammonium salt bactericidal agent with high-effective, broad-spectrum and low-toxic into sodium zirconium phosphate through an ion-exchange method.OBJECTIVE: To explore the component structure and antibacterial activity of quaternized zirconium phosphates.DESIGN, TIME AND SETTING: An in vitro observational experiment was performed at Research Laboratory of Department of Chemistry, Jinan University from June to August 2009.MATERIALS: Quaternized zirconium phosphates were prepared by introducing dodecyl dimethyl benzyl ammonium chloride into sodium zirconium phosphate through an ion-exchange method.METHODS: The mol ratios of quaternary ammonium cations to cation exchange capacity of sodium zirconium phosphate in reaction solutions were 0.25: 1,0.5: 1, 1.0: 1, and 1.5 : 1, respectively, and four kinds of quaternized zirconium phosphates containing different contents of quaternary ammonium cations (QZrP-1, QZrP-2, QZrP-3, QZrP-4) were prepared through an ion-exchange method.MAIN OUTCOME MEASURES: The component structure and heat resistance of samples were measured by using an IR spectrometer, an elemental analyzer and a thermal analyzer, respectively. The minimum inhibitory concentrations (MICs) and minimal bactericidal concentrations (MICs) of the samples against Escherichia coli (E. co/i) and Staphylococci aureus (S. aureus) were estimated by a tube broth method.RESULTS: Quaternized zirconium phosphates were prepared, and the quaternary ammonium cation content increased with increasing the concentration of quaternary ammonium cations in reaction solution. The mass fraction of quaternary ammonium cations of QZrP-1, QZrP-2, QZrP-3, and QZrP-4 was 3.70%, 5.00%, 6.96%, and 10.01%, respectively. The onset temperatures of the decomposition for quaternary ammonium cations in quaternized zirconium phosphates were all higher than 345 °C, and they were preferable thermal stability. The antibacterial activity was higher when the quaternary ammonium cation content of quaternized zirconium phosphates increased. For quaternized zirconium phosphates QZrP-3 containing 6.96% mass fraction of quaternary ammonium cations, showed excellent antibacterial activity against E. coli and S. aureus.CONCLUSION: Quaternized zirconium phosphates QZrP-3 containing 6.96% mass fraction of quaternary ammonium cations,exhibited excellent thermal stability and antibacterial activity.
