CAJ | 학술논문

目的:研究肝细胞癌(HCC)患者外周血CD95和OX40L mRNA的表达情况,并探讨其临床意义.方法:将研究对象的淋巴细胞与相应荧光标记的单克隆抗体反应,经洗涤、固定后,应用双色流式细胞术分析T淋巴细胞表面CD95~+CD3~+的表达量.采用荧光定量逆转录聚合酶链反应(FQ-RT-PCR)方法检测外周血单个核细胞共刺激分子OX40L mRNA的表达水平.结果:HCC患者外周血CD95~+CD3~+的表达水平明显高于健康对照组,差异有统计学意义[(34±20)%vs(20±7)%,t=2.960,P＜0.01];HCC患者外周血单个核细胞OX40L mRNA表达水平明显低于健康对照组,差异有统计学意义(0.46±0.36vs 0.86±0.70,t=2.302,P＜0.05).结论:HCC患者外周血CD95及OX40L分子的异常表达在HCC的发生中起重要作用.
목적:연구간세포암(HCC)환자외주혈CD95화OX40L mRNA적표체정황,병탐토기림상의의.방법:장연구대상적림파세포여상응형광표기적단극륭항체반응,경세조、고정후,응용쌍색류식세포술분석T림파세포표면CD95~+CD3~+적표체량.채용형광정량역전록취합매련반응(FQ-RT-PCR)방법검측외주혈단개핵세포공자격분자OX40L mRNA적표체수평.결과:HCC환자외주혈CD95~+CD3~+적표체수평명현고우건강대조조,차이유통계학의의[(34±20)%vs(20±7)%,t=2.960,P＜0.01];HCC환자외주혈단개핵세포OX40L mRNA표체수평명현저우건강대조조,차이유통계학의의(0.46±0.36vs 0.86±0.70,t=2.302,P＜0.05).결론:HCC환자외주혈CD95급OX40L분자적이상표체재HCC적발생중기중요작용.
Objective:To assess the expression levels of CD95 and OX40 ligand(OX40L)messenger RNA(mRNA)in hepatocellular carcinoma(HCC),and their clinical values thereof.Methods:The lymphocytes of research objects were mixed with corresponding fluorescence labeled monoclonal antibody(McAb);CD95 expression by CD3 positive T ceils was quantitatively measured by dual color flow cytometry.The expression of OX40L mRNA was detected in peripheral blood mononuclear cells by fluorescence quantitative reverse transcription polymerase chain reaction(FQ-RT-PCR).Results:The CD95 expression by CD3 positive T cells was significantly higher in patients with HCC(34±20)% compared with that of normal controls (20±7)%(t=2.960,P ＜ 0.01).The expression level of OX40L mRNA was significantly decreased in patients with HCC compared with that of normal controls(t=2.302,P ＜ 0.05).Conclusion:These results suggest that abnormal expressions of CD95 and OX40L play crucial roles in peripheral blood of HCC patients at the stage of human hepatocareinogenesis.