CAJ | 학술논문

为探讨紫杉烷类化合物的抗氧化应激作用,选择适当浓度双氧水处理神经母细胞瘤细胞(SK-N-SH)建立氧化应激细胞模型,用自红豆杉科的植物(Taxus cuspidate)中分离纯化的四种紫杉烷类化合物Taxine B、7-Deacetyl-taxine B、5-Cinnamoyloxy-taxin B、7,10-Diacetyl-2′-deoxyl-taxine A干预过氧化应激模型细胞,用四甲基偶氮唑盐(3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide,MTT)微量酶反应比色法筛选具有神经保护活性的化合物,Hoechst荧光染色观察细胞核形态改变,DCFH-DA检测细胞内活性氧含量.发现氧化应激模型细胞活力明显减低,应用7-Deacetyl-taxine B、5-Cinnamoyloxy-taxin B后细胞活力显著提高,细胞核形态改变明显减轻,细胞内活性氧含量明显减少.结果表明7-Deacetyl-taxine B、5-Cinnamoyloxy-taxin B可以减少细胞内氧自由基含量,减轻细胞核损伤,提高细胞活力.
위탐토자삼완류화합물적항양화응격작용,선택괄당농도쌍양수처리신경모세포류세포(SK-N-SH)건립양화응격세포모형,용자홍두삼과적식물(Taxus cuspidate)중분리순화적사충자삼완류화합물Taxine B、7-Deacetyl-taxine B、5-Cinnamoyloxy-taxin B、7,10-Diacetyl-2′-deoxyl-taxine A간예과양화응격모형세포,용사갑기우담서염(3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliumromide,MTT)미량매반응비색법사선구유신경보호활성적화합물,Hoechst형광염색관찰세포핵형태개변,DCFH-DA검측세포내활성양함량.발현양화응격모형세포활력명현감저,응용7-Deacetyl-taxine B、5-Cinnamoyloxy-taxin B후세포활력현저제고,세포핵형태개변명현감경,세포내활성양함량명현감소.결과표명7-Deacetyl-taxine B、5-Cinnamoyloxy-taxin B가이감소세포내양자유기함량,감경세포핵손상,제고세포활력.
Object:to study the anti-oxidative effects of taxanes,we used suitable concentration of hydrogen peroxide to establish the oxidative sk-n-sh cell model and added four taxanes compounds(Taxine B,7-Deacetyl-taxine B,5-Cinnamoyloxy-taxin B and 7,10-Diaectyl-2′-deoxyl-taxine A)to intervene the oxidative stress 2 hrs before hydrogen peroxide application.Their neuroprotective effects were evaluated using 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazoliummmide(MIT)assay,their effects of alleviating the nuclear morphological changes were detected after Hecchst 33258 staining and the activity ofdecreasing intracellular reactive oxygen species(Ros)was surveyed with flow cytometry.We discovered that 7-Deacetyl-taxine B and 5-Cinnamoyloxy-taxin B significantly improved cell viability,decreased obviously the number of cells with pycnosised neuclei and reduced intracellular ROS remarkably comparing with oxidative stress model.So we got the conclusion that 7-Deacetyl-taxine B,5-Cinnamoyloxy-taxin B can reduce intracellular ROS,protect cells neucleis and improve viability of oxidative model cells.