CAJ | 학술논문

目的:探讨广东地区汉族妇女核苷酸切除修复交叉互补基因1(ERCC1)基因单核苷酸多态位点C19007T(Asn118Asn,rs11615)与宫颈癌发生的关系,为宫颈癌的预防和治疗提供新的思路.方法:利用聚合酶链式反应.限制性片段长度多态性(PCR-RFLP)分析技术,对91例宫颈癌患者(宫颈癌组)和103例健康对照个体(对照组)的ERCC1 C19007T多态位点进行分型.采用非条件逻辑回归分析统计该多态位点与宫颈癌易感的相关性,并用年龄进行校正,计算相对危险度的比值比(OR)及95%置信区间(CI).结果:T等位基因在宫颈癌组中的分布频率(31.3%)高于对照组(20.4%),差异有统计学意义(P=0.024),携带,IT基因型的个体显著增加患宫颈癌的风险(调整后的OR=3.68,95%CI=1.22～11.14,P=0.021).而且T等位基因增加个体患宫颈癌风险的趋势在Ⅰ期的宫颈癌患者(TT vs CC:调整后的OR=4.69,95%CI=1.36～16.21)以及在<45岁的人群中(TTT vs CC:调整后的OR=5.05,95%CI=1.01～25.20)更加明显.结论:ERCCl C19007T多态与广东地区汉族妇女宫颈癌的发生有密切关系,T等位基因可能是影响宫颈癌易感的一个风险因素.
목적:탐토엄동지구한족부녀핵감산절제수복교차호보기인1(ERCC1)기인단핵감산다태위점C19007T(Asn118Asn,rs11615)여궁경암발생적관계,위궁경암적예방화치료제공신적사로.방법:이용취합매련식반응.한제성편단장도다태성(PCR-RFLP)분석기술,대91례궁경암환자(궁경암조)화103례건강대조개체(대조조)적ERCC1 C19007T다태위점진행분형.채용비조건라집회귀분석통계해다태위점여궁경암역감적상관성,병용년령진행교정,계산상대위험도적비치비(OR)급95%치신구간(CI).결과:T등위기인재궁경암조중적분포빈솔(31.3%)고우대조조(20.4%),차이유통계학의의(P=0.024),휴대,IT기인형적개체현저증가환궁경암적풍험(조정후적OR=3.68,95%CI=1.22～11.14,P=0.021).이차T등위기인증가개체환궁경암풍험적추세재Ⅰ기적궁경암환자(TT vs CC:조정후적OR=4.69,95%CI=1.36～16.21)이급재<45세적인군중(TTT vs CC:조정후적OR=5.05,95%CI=1.01～25.20)경가명현.결론:ERCCl C19007T다태여엄동지구한족부녀궁경암적발생유밀절관계,T등위기인가능시영향궁경암역감적일개풍험인소.
Objective:To investigate the relationship between the excision repair cross-complementing group 1 (ERCC1) C19007T polymorphism and the risk of cervical cancer in Guangdong Chinese Han women, which provide a new thread for prevention and treatment of cervical cancer.Methods:The geno-types of the ERCC1 C19007T polymorphism were determined in 91 cervical cancer patients (ceNical cancer group) and 103 heatthy female blood donors (control group) by polymerase chain reaction-based restriction fragment length polymorphism (PCR-RFLP).Association between the polymorphism and the risk for cervical cancer was evaluated using unconditionallogistic regression analysis, adjusted by age.Odds Ration (OR) of relative nsk and 95% confidence inteNal(CI) were calculated.Results :The distribution frequency of 19007T allele was higher in cervical cancer group (31.3%) than that in control group (20.4%) , which was signifi-cant differences(P = 0.024).Compared with the ERCC1 19007CC homozygotes, the homozygous TI-gen-otype (adjusted OR =3.68 ,95% CI =1.22 ～11.14, P = 0.021) were statistically significantly associated with the increased nsk of cervical cancer.Moreover, the increased risk of developing cervical cancer conferred by the T allele was more pronounced in patients at stage Ⅰ (TT vs CC :adjusted OR = 4.69,95%CI =1.36 ～ 16.21) or in the individuals younger than 45 years old (TT vs CC:adjusted OR = 5.05, 95% CI = 1.01 ～ 25.20).Conclusions:ERCC1 C19007T polymorphism is closely related to the development of ceNical cancer in Guangdong Chinese Han women, and 19007T allelic variation might be an important risk factor for ceNical cancar.