中华传染病杂志
中華傳染病雜誌
중화전염병잡지
CHINESE JOURNAL OF INFECTIOUS DISEASES
2008年
6期
345-349
,共5页
孙静慧%张慧%耿红莲%刘皋林%孔宪涛%谭龙益%TAN Long-yi
孫靜慧%張慧%耿紅蓮%劉皋林%孔憲濤%譚龍益%TAN Long-yi
손정혜%장혜%경홍련%류고림%공헌도%담룡익%TAN Long-yi
La蛋白%RNA%小分子干扰%肝炎病毒%乙型%基因表达
La蛋白%RNA%小分子榦擾%肝炎病毒%乙型%基因錶達
La단백%RNA%소분자간우%간염병독%을형%기인표체
La protein%RNA,small interfering%Hepatitis B virus%Gene expression
目的 探讨细胞内La蛋白对HBV蛋白质表达的影响.方法 针对人La蛋白序列设计特异性的小分子干扰RNA(siRNA),通过体外转录的方法合成双链siRNA,并转染进入稳定表达HBV的HepG2.2.15细胞,实时荧光定量基因扩增技术测定HepG2.2.15细胞中的La蛋白mRNA变化水平;电化学发光分析技术检测HepG2.2.15细胞分泌的HBsAg和HBeAg变化情况.所得数据进行配对假设检验的统计学分析.结果 特异性siRNA干扰La蛋白mRNA的表达,使La蛋白在HepG2.2.15细胞中的表达降低;HepG2.2.15细胞分泌在培养上清液中的HBsAg和HBeAg表达显著下降,相关性分析显示,HBsAg、HBeAg变化水平与La蛋白mRNA变化水平呈正相关,相关系数分别为0.938和0.899.结论 特异性siRNA能够抑制细胞内La蛋白mRNA的表达,La蛋白可通过某种方式影响HBV蛋白质的表达.
目的 探討細胞內La蛋白對HBV蛋白質錶達的影響.方法 針對人La蛋白序列設計特異性的小分子榦擾RNA(siRNA),通過體外轉錄的方法閤成雙鏈siRNA,併轉染進入穩定錶達HBV的HepG2.2.15細胞,實時熒光定量基因擴增技術測定HepG2.2.15細胞中的La蛋白mRNA變化水平;電化學髮光分析技術檢測HepG2.2.15細胞分泌的HBsAg和HBeAg變化情況.所得數據進行配對假設檢驗的統計學分析.結果 特異性siRNA榦擾La蛋白mRNA的錶達,使La蛋白在HepG2.2.15細胞中的錶達降低;HepG2.2.15細胞分泌在培養上清液中的HBsAg和HBeAg錶達顯著下降,相關性分析顯示,HBsAg、HBeAg變化水平與La蛋白mRNA變化水平呈正相關,相關繫數分彆為0.938和0.899.結論 特異性siRNA能夠抑製細胞內La蛋白mRNA的錶達,La蛋白可通過某種方式影響HBV蛋白質的錶達.
목적 탐토세포내La단백대HBV단백질표체적영향.방법 침대인La단백서렬설계특이성적소분자간우RNA(siRNA),통과체외전록적방법합성쌍련siRNA,병전염진입은정표체HBV적HepG2.2.15세포,실시형광정량기인확증기술측정HepG2.2.15세포중적La단백mRNA변화수평;전화학발광분석기술검측HepG2.2.15세포분비적HBsAg화HBeAg변화정황.소득수거진행배대가설검험적통계학분석.결과 특이성siRNA간우La단백mRNA적표체,사La단백재HepG2.2.15세포중적표체강저;HepG2.2.15세포분비재배양상청액중적HBsAg화HBeAg표체현저하강,상관성분석현시,HBsAg、HBeAg변화수평여La단백mRNA변화수평정정상관,상관계수분별위0.938화0.899.결론 특이성siRNA능구억제세포내La단백mRNA적표체,La단백가통과모충방식영향HBV단백질적표체.
Objective To prove that La protein can effect the expressions of hepatitis B virus (HBV) of protein in cultured cells.Methods Three specific small interfering RNAs (siRNAs) were obtained by transcription in vitro.After transfection with the siRNAs into HepG2.2.15 cell,the levels of La protein mRNA were detected by real-time polymerase chain reaction(PCR),the quantities of HBsAg and HBeAg were analyzed by Electroehemilunescence immunoassay (ECL).All datas were analyzed by hypothesis testing.Results mRNA of La protein was reduced in HepG2.2.15 cells transfected with the siRNAs,which was accompanies with reductions of both HBsAg and HBeAg secreted by these cells.Association analysis suggested the changes of both HBsAg and HBeAg were positively correlated with mRNA level of La protein (r=O.938,r=0.899).Conclusion Specific siRNAs can effect the expression of the La protein,which can in turn affect the expressions of protein of HBV in cultured HepG2.2.15 cells.
