CAJ | 학술논문

目的探讨当归注射液对大鼠慢性肺动脉高压的影响.方法 30只雄性Sprague-Dawley大鼠随机分成对照组、低氧组、当归保护组,每组10只.当归保护组每天缺氧前腹腔注射25%当归注射液[10mg/(kg · d)];对照组、低氧组于每天缺氧前腹腔注射等量生理盐水.常压低氧建立大鼠肺动脉高压模型,4周末测各组大鼠平均肺动脉压(mPAP)、应用免疫组织化学法和图像分析技术定量检测大鼠肺小动脉管壁和内皮细胞增殖细胞核抗原(proliferating cell nuclear antigen,PCNA)、诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)的表达水平;同时肺小动脉行HE染色,测定肺小动脉管壁面积占肺小动脉总面积的百分比(wA%).结果对照组、低氧组、当归保护组的mPAP分别为10.50±1.90、35.36±9.11、18.32±2.30(mm Hg);wA%分别为52.71±5.16、82.38±8.43、64.58±9.54(%),低氧组的mPAP、wA%均高于对照组(P<0.01),而当归保护组mPAP、wA%均明显低于低氧组(P<0.01).对照组、低氧组、当归保护组肺小动脉PCNA表达水平分别为3.15±1.10、24.50±5.72、12.67±3.46(%),缺氧组显著高于对照组(P<0.01),而当归保护组明显低于低氧组(P<0.01).对照组、低氧组、当归保护组肺小动脉血管内皮iNOS蛋白表达水平分别为2.13±1.01、17.33±3.53、37.50±7.04(%),低氧组显著高于对照组(P<0.01),而当归保护组明显高于低氧组和对照组(P<0.01).结论当归注射液可以降低慢性低氧所致肺动脉高压,其机制与抑制肺小动脉PCNA表达、增加肺小动脉iNOS表达水平有关.
목적 탐토당귀주사액대대서만성폐동맥고압적영향.방법 30지웅성Sprague-Dawley대서수궤분성대조조、저양조、당귀보호조,매조10지.당귀보호조매천결양전복강주사25%당귀주사액[10mg/(kg · d)];대조조、저양조우매천결양전복강주사등량생리염수.상압저양건립대서폐동맥고압모형,4주말측각조대서평균폐동맥압(mPAP)、응용면역조직화학법화도상분석기술정량검측대서폐소동맥관벽화내피세포증식세포핵항원(proliferating cell nuclear antigen,PCNA)、유도형일양화담합매(inducible nitric oxide synthase,iNOS)적표체수평;동시폐소동맥행HE염색,측정폐소동맥관벽면적점폐소동맥총면적적백분비(wA%).결과 대조조、저양조、당귀보호조적mPAP분별위10.50±1.90、35.36±9.11、18.32±2.30(mm Hg);wA%분별위52.71±5.16、82.38±8.43、64.58±9.54(%),저양조적mPAP、wA%균고우대조조(P<0.01),이당귀보호조mPAP、wA%균명현저우저양조(P<0.01).대조조、저양조、당귀보호조폐소동맥PCNA표체수평분별위3.15±1.10、24.50±5.72、12.67±3.46(%),결양조현저고우대조조(P<0.01),이당귀보호조명현저우저양조(P<0.01).대조조、저양조、당귀보호조폐소동맥혈관내피iNOS단백표체수평분별위2.13±1.01、17.33±3.53、37.50±7.04(%),저양조현저고우대조조(P<0.01),이당귀보호조명현고우저양조화대조조(P<0.01).결론 당귀주사액가이강저만성저양소치폐동맥고압,기궤제여억제폐소동맥PCNA표체、증가폐소동맥iNOS표체수평유관.
Objective To investigate the effects of angelica solution on chronic hypoxic pulmonary hypertension in rats.Methods Thirty SD rats were randomized into normoxic group,hypoxic group and angelica solution-protected group.The model of rat chronic hypoxic pulmonary hypertension was made by method of isobaric hypoxia.Angelica solution were injected before hypoxia,while the other two groups were injected normal saline.After 28d of hypoxia,pulmonary artery pressure were measured.Expressions of proliferating cell nuclear antigen (PCNA) and inducible nitric oxide synthase (iNOS) in pulmonary artery were detected by immunohistochemical staining.The index of wall thickness of rat pulmonary arteriole-percentage of the wall area in the total vascular area(wA%) were measured by a computerized image analyzer.Results The mean pulmonary artery pressure (mPAP) of normoxic group,hypoxic group and angelica solution-protected group were10.50±1.90,35.36±9.11,18.32±2.30 (mm Hg);wA% of the three groups were 52.71±5.16,82.38±8.43,64.58±9.54 (%),mPAP and wA% were significantly higher in the hypoxic group than those in the normoxic group (P<0.01) and angelica solution-protected group (P<0.01).PCNA expression of normoxic group,hypoxic group and angelica solution-protected group were 3.15±1.10,24.50±5.72,12.67±3.46 (%).The PCNA expression in the pulmonary artery was significantly higher in the hypoxic group than those in the normoxic group (P<0.01) and in the angelica solution-protected group (P<0.01).iNOS expression of normoxic group,hypoxic group and angelica solution-protected group were 2.13±1.01,17.33±3.53,37.50±7.04 (%).iNOS expression in the pulmonary artery was higher in the hypoxic group than those in normoxic group (P<0.01),and angelica significantly increased iNOS expression in comparison with the normoxic and hypoxic groups (P<0.01).Conclusion Angelica solution alleviates chronically hypoxia induced pulmonary hypertension in rats by inhibiting the espression of PCNA in pulmonary artery and up-regulating the expression of iNOS.