中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
1期
123-125
,共3页
胡祥%喻爱喜%余国荣%陶圣祥%夏成焱%吴刚
鬍祥%喻愛喜%餘國榮%陶聖祥%夏成焱%吳剛
호상%유애희%여국영%도골상%하성염%오강
负压封闭引流%细胞黏附分子-1%核因子-κB%IκBα%创面
負壓封閉引流%細胞黏附分子-1%覈因子-κB%IκBα%創麵
부압봉폐인류%세포점부분자-1%핵인자-κB%IκBα%창면
Vacuum sealing drainage%Intercellular adhesion molecule-1%IκBα%Nuclear factor-kappa B%Wound
目的 观察负压封闭引流技术(VSD)对创面细胞黏附分子-1(ICAM-1)表达及IκBα 磷酸化的影响.方法 每组建立24例Sus scrafa猪背急性感染创面模型,VSD治疗为实验组,120 mm Hg(1 mm Hg =0.133 kPa)负压持续吸引,常规换药治疗为对照组,对创面炎性反应、ICAM-1 表达及IκBα磷酸化的水平进行分析.结果 与对照组比较,VSD组减轻感染创面炎性反应,ICAM-1 表达相对值0.60±0.08,显著低于对照组1.01±0.06(P ＜0.05),VSD显著减少IκBα磷酸化(40±9)％,(P＜0.05).结论 负压封闭引流减少IκBα磷酸化,抑制感染创面ICAM-1的表达,明显减轻感染创面炎性反应.
目的 觀察負壓封閉引流技術(VSD)對創麵細胞黏附分子-1(ICAM-1)錶達及IκBα 燐痠化的影響.方法 每組建立24例Sus scrafa豬揹急性感染創麵模型,VSD治療為實驗組,120 mm Hg(1 mm Hg =0.133 kPa)負壓持續吸引,常規換藥治療為對照組,對創麵炎性反應、ICAM-1 錶達及IκBα燐痠化的水平進行分析.結果 與對照組比較,VSD組減輕感染創麵炎性反應,ICAM-1 錶達相對值0.60±0.08,顯著低于對照組1.01±0.06(P ＜0.05),VSD顯著減少IκBα燐痠化(40±9)％,(P＜0.05).結論 負壓封閉引流減少IκBα燐痠化,抑製感染創麵ICAM-1的錶達,明顯減輕感染創麵炎性反應.
목적 관찰부압봉폐인류기술(VSD)대창면세포점부분자-1(ICAM-1)표체급IκBα 린산화적영향.방법 매조건립24례Sus scrafa저배급성감염창면모형,VSD치료위실험조,120 mm Hg(1 mm Hg =0.133 kPa)부압지속흡인,상규환약치료위대조조,대창면염성반응、ICAM-1 표체급IκBα린산화적수평진행분석.결과 여대조조비교,VSD조감경감염창면염성반응,ICAM-1 표체상대치0.60±0.08,현저저우대조조1.01±0.06(P ＜0.05),VSD현저감소IκBα린산화(40±9)％,(P＜0.05).결론 부압봉폐인류감소IκBα린산화,억제감염창면ICAM-1적표체,명현감경감염창면염성반응.
Objective To investigate the effect of vacuum sealing drainage (VSD) on intercellular adhesion molecule-1 ( ICAM-1 ) and related mechanism in wound healing.Methods Models of acute infected wound were made in each 24 standard samples on back of Sus scrafa pigs,and VSD was performed as treated group with - 120 mm Hg ( 1 mm Hg =0.133 kPa),while common dressing as control group.Wound was assessed by Hematoxylin and Eoain (HE) stain at the 7th day after treatment.The expression of ICAM-1 was detected by using immunochemistry,real-time polymerase chain reaction (PCR) and Western blotting in granulation tissues as well as the p-IκBαt and IκBα by using Western blotting.Results ICAM-1 in the VSD group was 0.60 ±0.08,lower than the control group (1.0l ±0.06),P＜0.05; the protein level of p-IκBα was decreased by (40 ±9)％ as well as p-IκBα/IκBα in VSD group as compared with control group (P ＜ 0.05 ).Conclusion VSD obviously decreases the expression of ICAM-1 via suppressing IκBα phosphorylation in the wound healing.
