CAJ | 학술논문

目的研究常温时液压冲击伤后神经元内依钙蛋白酶活性变化及亚低温的干预效果. 方法体外培养大鼠神经元并制作液压冲击伤模型,在不同时间点进行亚低温干预,以紫外分光光度法检测液压冲击伤后的神经元内Calpain的活性变化及在不同时段亚低温对Calpain活性的干预效应. 结果常温时,在细胞损伤后Calpain的活性发生显著变化.亚低温组与常温组Calpain的活性不同,且亚低温对伤后Calpain活性的干预效应与作用起始时间密切相关. 结论液压冲击伤后神经元内Calpain活性发生的改变参与细胞创伤的病理过程,亚低温可能通过这一机制具有一定保护创伤性脑损伤的作用.亚低温干预的效果与开始干预时间相关.
목적 연구상온시액압충격상후신경원내의개단백매활성변화급아저온적간예효과. 방법 체외배양대서신경원병제작액압충격상모형,재불동시간점진행아저온간예,이자외분광광도법검측액압충격상후적신경원내Calpain적활성변화급재불동시단아저온대Calpain활성적간예효응. 결과 상온시,재세포손상후Calpain적활성발생현저변화.아저온조여상온조Calpain적활성불동,차아저온대상후Calpain활성적간예효응여작용기시시간밀절상관. 결론 액압충격상후신경원내Calpain활성발생적개변삼여세포창상적병리과정,아저온가능통과저일궤제구유일정보호창상성뇌손상적작용.아저온간예적효과여개시간예시간상관.
Objective To investigate time course changes of intraneuronal calpain activity following fluid percussion injury(FPI)under normothermia(37℃)and mild hypothermia(32+0.5)℃.Methods Rats' neurons produced by external culture and FPI models prepared, mild hypothermia was applied for intervention at different time points and the changes in intraneuronal calpain activity following FPI and the intervention effect of mild hypothermia on calpain activity during different time courses were detected by UV-spectrophotometry. Results Remarkable changes occurred to calpain activity following FPI at 37℃. While the intervention effect of mild hypothermia on calpain activity was different, closely related to the starting time point of intervention. Conclusion The changes of intraneuronal calpain activity following FPI join the pathological process of cellular trauma; mild hypothermia might protect patients with traumatic brain injury(TBI)to some extent through the regulation of calpain activity. And, the intervention effect of mild hypothermia has some correlation with the starting time point of intervention.