中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2010年
9期
775-779
,共5页
甄森%张济%马衍辉%张宁%刘岚铮%王天成%陈春英%周敬文%李新伟%钱琴%吕艳朋%林少倩%贾光
甄森%張濟%馬衍輝%張寧%劉嵐錚%王天成%陳春英%週敬文%李新偉%錢琴%呂豔朋%林少倩%賈光
견삼%장제%마연휘%장저%류람쟁%왕천성%진춘영%주경문%리신위%전금%려염붕%림소천%가광
钛%氧化合物%职业暴露%脱氧鸟苷%定群研究
鈦%氧化閤物%職業暴露%脫氧鳥苷%定群研究
태%양화합물%직업폭로%탈양조감%정군연구
Titanium%Oxygen compounds%Occupational exposure%Deoxyguanosine%Panel study
目的 了解职业人群吸入性二氧化钛颗粒的接触水平,探讨其对工人机体氧化应激水平的影响.方法 应用定群设计的研究方法 ,通过问卷调查了解某二氧化钛生产车间成品岗位7名工人的一般信息及职业接触史.观察前后分别采集工人外周静脉血10ml,同时,连续29 d每日班前班后30 min内各采集尿样30 ml(共60 ml),并测定工人每日二氧化钛接触量及车间环境温、湿度.乳胶免疫比浊法测定个体外周血清超敏C反应蛋白(high-sensitivity C-reactive protein,hs-CRP)水平;酶联免疫吸附法(ELISA)测定尿8-羟基脱氧鸟苷(8-hydroxy-deoxyguanosine,8-OHdG)水平.结果 个体每日接触可吸入性二氧化钛颗粒平均浓度为(1. 194±1.015)mg/m3;观察前后血样中hs-CRP水平分别为(1.13±1.08)、(1.33±1.01)mg/L,差异未见统计学意义(t=-0.848,P=0.425);班前班后尿样中8-OHdG水平分别为(3.51±1.39)、(3.65±1.06)μmol/mol Cr.相关性分析显示,随着二氧化钛颗粒接触浓度的增加,8-OHdG水平班前班后差异呈增大趋势(r=0.192,t=2.09,P=0.039).经班次、工龄、年龄、体质指数(BMI)等调整后,单污染物模型分析未发现二氧化钛浓度与班前班后8-OHdG水平差异存在统计学意义(β=0.288,t=1.940,P=0.055).结论 职业场所可吸入二氧化钛颗粒在所研究浓度范围,尚未发现工人机体DNA氧化应激水平发生明显变化.
目的 瞭解職業人群吸入性二氧化鈦顆粒的接觸水平,探討其對工人機體氧化應激水平的影響.方法 應用定群設計的研究方法 ,通過問捲調查瞭解某二氧化鈦生產車間成品崗位7名工人的一般信息及職業接觸史.觀察前後分彆採集工人外週靜脈血10ml,同時,連續29 d每日班前班後30 min內各採集尿樣30 ml(共60 ml),併測定工人每日二氧化鈦接觸量及車間環境溫、濕度.乳膠免疫比濁法測定箇體外週血清超敏C反應蛋白(high-sensitivity C-reactive protein,hs-CRP)水平;酶聯免疫吸附法(ELISA)測定尿8-羥基脫氧鳥苷(8-hydroxy-deoxyguanosine,8-OHdG)水平.結果 箇體每日接觸可吸入性二氧化鈦顆粒平均濃度為(1. 194±1.015)mg/m3;觀察前後血樣中hs-CRP水平分彆為(1.13±1.08)、(1.33±1.01)mg/L,差異未見統計學意義(t=-0.848,P=0.425);班前班後尿樣中8-OHdG水平分彆為(3.51±1.39)、(3.65±1.06)μmol/mol Cr.相關性分析顯示,隨著二氧化鈦顆粒接觸濃度的增加,8-OHdG水平班前班後差異呈增大趨勢(r=0.192,t=2.09,P=0.039).經班次、工齡、年齡、體質指數(BMI)等調整後,單汙染物模型分析未髮現二氧化鈦濃度與班前班後8-OHdG水平差異存在統計學意義(β=0.288,t=1.940,P=0.055).結論 職業場所可吸入二氧化鈦顆粒在所研究濃度範圍,尚未髮現工人機體DNA氧化應激水平髮生明顯變化.
목적 료해직업인군흡입성이양화태과립적접촉수평,탐토기대공인궤체양화응격수평적영향.방법 응용정군설계적연구방법 ,통과문권조사료해모이양화태생산차간성품강위7명공인적일반신식급직업접촉사.관찰전후분별채집공인외주정맥혈10ml,동시,련속29 d매일반전반후30 min내각채집뇨양30 ml(공60 ml),병측정공인매일이양화태접촉량급차간배경온、습도.유효면역비탁법측정개체외주혈청초민C반응단백(high-sensitivity C-reactive protein,hs-CRP)수평;매련면역흡부법(ELISA)측정뇨8-간기탈양조감(8-hydroxy-deoxyguanosine,8-OHdG)수평.결과 개체매일접촉가흡입성이양화태과립평균농도위(1. 194±1.015)mg/m3;관찰전후혈양중hs-CRP수평분별위(1.13±1.08)、(1.33±1.01)mg/L,차이미견통계학의의(t=-0.848,P=0.425);반전반후뇨양중8-OHdG수평분별위(3.51±1.39)、(3.65±1.06)μmol/mol Cr.상관성분석현시,수착이양화태과립접촉농도적증가,8-OHdG수평반전반후차이정증대추세(r=0.192,t=2.09,P=0.039).경반차、공령、년령、체질지수(BMI)등조정후,단오염물모형분석미발현이양화태농도여반전반후8-OHdG수평차이존재통계학의의(β=0.288,t=1.940,P=0.055).결론 직업장소가흡입이양화태과립재소연구농도범위,상미발현공인궤체DNA양화응격수평발생명현변화.
Objective To investigate the inhalable titanium dioxide exposure level and make an assessment of its oxidative effect on occupational exposed population. Methods A total of 7 workers occupationally exposing to inhalable titanium dioxide were recruited into the study. The basic information and occupational history were collected by interview, while their blood sample (10 ml for each subject) were collected before and after the investigation, respectively. Pre- and post-work shift urine samples (60 ml for each subject) were collected for 29 days consecutively. The daily personal titanium dioxide exposure level,temperature and relative humidity were detected too. Urinary 8-hydroxy-deoxyguanosine (8-OHdG) and serum high-sensitivity C-reactive protein (hs-CRP) were detected by ELISA and latex immunoturbidimetric assay,respectively. Results The mean concentration of air inhalable titanium dioxide was (1. 194 ±1. 015)mg/m3. Serum hs-CRP level before and after the investigation was (1.13 ± 1.08), (1.33 ±1.01)mg/L,respectively. No statistical significance was observed between hs-CRP level before and after the investigation(t = - 0. 848, P = 0. 425). Pre- and post-work shift urinary 8-OHdG was (3.51 ± 1.39),(3.65 ± 1.06)μmol/mol Cr, respectively. A positive correlation was found between the concentration of inhalable titanium dioxide and the changes of 8-OHdG level (r = 0. 192, t = 2. 09, P = 0. 039). Linear mixed-effect models, adjusted by work shift, years of employment, age, body mass index, smoking status,temperature and relative humidity, showed no significant exposure-respond trend between the inhalable titanium dioxide concentration and 8-OHdG level (β = 0. 288, t = 1. 940, P = 0. 055). Conclusion Our findings do not support the potential link between occupationally exposure to inhalable titanium dioxide and high induction of DNA oxidative stress.
