中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2008年
7期
522-526
,共5页
蒋洪彦%王世鑫%李雪华%魏茂提%杨霞%陈娟娟%栾大伟%李哲%陈义辉%翁亚光
蔣洪彥%王世鑫%李雪華%魏茂提%楊霞%陳娟娟%欒大偉%李哲%陳義輝%翁亞光
장홍언%왕세흠%리설화%위무제%양하%진연연%란대위%리철%진의휘%옹아광
严重急性呼吸综合征%股骨头坏死%蛋白质组学%生物标记物
嚴重急性呼吸綜閤徵%股骨頭壞死%蛋白質組學%生物標記物
엄중급성호흡종합정%고골두배사%단백질조학%생물표기물
Severe acute respiratory syndrome%Femur head necrosis%Proteomics%Biomarker
目的 分析严重急性呼吸综合征(SARS)康复人员并发股骨头缺血性坏死血清差异蛋白,探讨其发生机制.方法 设立正常健康女性人员为正常对照组(A组,10例)、SARS康复无并发症的女性人员为单纯组(B组,10例)、SARS康复并发股骨头缺血性坏死的女性人员为坏死组(c组,10例).应用双向凝胶电泳结合基质辅助激光解吸飞行时间串联质谱技术对A、B、C组进行血清比较蛋白质组学研究;酶联免疫吸附测定血清淀粉样P成分在3个组个体血清样本中的表达;统计分析其特异度和敏感度.结果 A、B、c组双向电泳凝胶图蛋白质点数分别为632±28、671±55、688±42.匹配率为85%~95%;共找出18个差异蛋白,经质谱鉴定为甲状腺素运载蛋白、丝氨酸蛋白酶抑制因子、α-1抗胰蛋白酶前体、血清淀粉样P成分等10种差异蛋白;与A组和B组比较,C组甲状腺素运载蛋白A链、白蛋白、前白蛋白、补体C4、纤维蛋白原γ载脂蛋白L低表达,而丝氨酸蛋白酶抑制因子、α-1抗胰蛋白酶前体、血清淀粉样P成分高表达;血清淀粉样P成分在A、B、C组个体血清样本中的含量分别为(0.54±0.30)、(0.83±0.39)、(1.21±0.29)ns/ml;与A组和B组比较,C组血清淀粉样P成分高表达(t值分别为2.27、2.98,P<0.05);ROC曲线下面积为0.854,特异度77.8%,.敏感度85.2%.结论 3组间存在差异表达蛋白,血清淀粉样P成分可能成为SARS康复人员并发股骨头缺血性坏死潜在血清标记物之一.
目的 分析嚴重急性呼吸綜閤徵(SARS)康複人員併髮股骨頭缺血性壞死血清差異蛋白,探討其髮生機製.方法 設立正常健康女性人員為正常對照組(A組,10例)、SARS康複無併髮癥的女性人員為單純組(B組,10例)、SARS康複併髮股骨頭缺血性壞死的女性人員為壞死組(c組,10例).應用雙嚮凝膠電泳結閤基質輔助激光解吸飛行時間串聯質譜技術對A、B、C組進行血清比較蛋白質組學研究;酶聯免疫吸附測定血清澱粉樣P成分在3箇組箇體血清樣本中的錶達;統計分析其特異度和敏感度.結果 A、B、c組雙嚮電泳凝膠圖蛋白質點數分彆為632±28、671±55、688±42.匹配率為85%~95%;共找齣18箇差異蛋白,經質譜鑒定為甲狀腺素運載蛋白、絲氨痠蛋白酶抑製因子、α-1抗胰蛋白酶前體、血清澱粉樣P成分等10種差異蛋白;與A組和B組比較,C組甲狀腺素運載蛋白A鏈、白蛋白、前白蛋白、補體C4、纖維蛋白原γ載脂蛋白L低錶達,而絲氨痠蛋白酶抑製因子、α-1抗胰蛋白酶前體、血清澱粉樣P成分高錶達;血清澱粉樣P成分在A、B、C組箇體血清樣本中的含量分彆為(0.54±0.30)、(0.83±0.39)、(1.21±0.29)ns/ml;與A組和B組比較,C組血清澱粉樣P成分高錶達(t值分彆為2.27、2.98,P<0.05);ROC麯線下麵積為0.854,特異度77.8%,.敏感度85.2%.結論 3組間存在差異錶達蛋白,血清澱粉樣P成分可能成為SARS康複人員併髮股骨頭缺血性壞死潛在血清標記物之一.
목적 분석엄중급성호흡종합정(SARS)강복인원병발고골두결혈성배사혈청차이단백,탐토기발생궤제.방법 설립정상건강녀성인원위정상대조조(A조,10례)、SARS강복무병발증적녀성인원위단순조(B조,10례)、SARS강복병발고골두결혈성배사적녀성인원위배사조(c조,10례).응용쌍향응효전영결합기질보조격광해흡비행시간천련질보기술대A、B、C조진행혈청비교단백질조학연구;매련면역흡부측정혈청정분양P성분재3개조개체혈청양본중적표체;통계분석기특이도화민감도.결과 A、B、c조쌍향전영응효도단백질점수분별위632±28、671±55、688±42.필배솔위85%~95%;공조출18개차이단백,경질보감정위갑상선소운재단백、사안산단백매억제인자、α-1항이단백매전체、혈청정분양P성분등10충차이단백;여A조화B조비교,C조갑상선소운재단백A련、백단백、전백단백、보체C4、섬유단백원γ재지단백L저표체,이사안산단백매억제인자、α-1항이단백매전체、혈청정분양P성분고표체;혈청정분양P성분재A、B、C조개체혈청양본중적함량분별위(0.54±0.30)、(0.83±0.39)、(1.21±0.29)ns/ml;여A조화B조비교,C조혈청정분양P성분고표체(t치분별위2.27、2.98,P<0.05);ROC곡선하면적위0.854,특이도77.8%,.민감도85.2%.결론 3조간존재차이표체단백,혈청정분양P성분가능성위SARS강복인원병발고골두결혈성배사잠재혈청표기물지일.
Objective To seek differentially expressed serum proteins in recovered SARS patients complicating avascular necrosis of femoral head (AVNFH). Methods 2-DE and MALDI-TOF MS were used to study the comparative serum proteomics among female SARS AVNFH group, female SARS non-AVNFH group and female healthy group. ELISA method was used to detect serum amyloid P component in individual serum: specificity and sensitivity of serum amyloid P component were analyzed. Results Average protein points on 2-DE of 3 groups were 632±28,671±55,688±42 respectively, and the matching rate of protein points was ranged from 85%to 95%;eighteen differentially expressed proteins were discovered including transthyretin, serpin peptidase inhibitor,alpha-1-antitrypsin precursor, serum amyloid Pcomponents, etc. Compared to healthy group and SARS non-AVNFH group,transthyretin,CAB3,fibrinogengamma,apolipoprotein L, apolipoprotein A一1V precursor, albumin and prealbumin showed lower expression, inversely serpin peptidase inhibitor,alpha-1-antitrypsin precursor and serum amyloid P components showeds higher expression in serum in the SARS AVNFH necrosis group. The serum amyloid P component in 3 groups were 0.54±0.30 ng/m1.0.83±0.39 ng/m1.I.21±0.29 rig/ml respectively. The areas under the ROC curve on serum amyloid P component was 0.854,the specificity was 77.8%and the sensitivity was 85.2%. Conclusion There were differentially expressed serum proteins in three groups. Serum amyloid P components might be one of the potential biomarders in serum of recovered SARS patients complicating avascular necrosis of femoral head.