CAJ | 학술논문

通过对分别来自四川、湖北、浙江、上海的麦冬(Ophiopogon japonicus(L.f.)Ker-Gawl.)的13个居群和两个沿阶草种(Ophiopogon bodinieri Lévl和Ophiopogon bockianus Diels)的RAPD分析,研究麦冬的道地性.所用麦冬和两个近缘种同属沿阶草属.从40条随机引物中筛选到8条引物用作RAPD分析.通过分析获得了504条RAPD带,其中多态性占89.4%.根据距离系数构建了13个麦冬居群和两个沿阶草种的非加权算术平均(UPGMA)树.最大的遗传距离是在麦冬居群和两个沿阶草种间.在麦冬种中,采自四川大学的大叶型与其他居群距离最大.在不同地理分布和不同叶型的麦冬之间具有高度的遗传多样性.证明RAPD是一个能有效区分麦冬与其他同属物种以及麦冬道地性分析的方法.
통과대분별래자사천、호북、절강、상해적맥동(Ophiopogon japonicus(L.f.)Ker-Gawl.)적13개거군화량개연계초충(Ophiopogon bodinieri Lévl화Ophiopogon bockianus Diels)적RAPD분석,연구맥동적도지성.소용맥동화량개근연충동속연계초속.종40조수궤인물중사선도8조인물용작RAPD분석.통과분석획득료504조RAPD대,기중다태성점89.4%.근거거리계수구건료13개맥동거군화량개연계초충적비가권산술평균(UPGMA)수.최대적유전거리시재맥동거군화량개연계초충간.재맥동충중,채자사천대학적대협형여기타거군거리최대.재불동지리분포화불동협형적맥동지간구유고도적유전다양성.증명RAPD시일개능유효구분맥동여기타동속물충이급맥동도지성분석적방법.
To identify Ophiopogon japonicus (L. f. ) Ker-Gawl. as geo-herbal, 13 populations of O. japonicus (L. f. ) Ker-Gawl. and two other species, Ophiopogon bodinieri L évl and Ophiopogon bockianus Diels, were investigated using the random amplified polymorphic DNA (RAPD) method. All natural populations of O. japonicus (L. f. ) Ker-Gawl. from Sichuan, Hubei, Zhejiang, Shanghai, and two other species belong to the same genus Ophiopogon. Eight RAPD primers selected from 40 random decamer oligonucleotides were used for the analysis. Five hundred and four RAPD bands were generated by the 8 selected primers and the percentage of polymorphic bands was 89.4% on average. The 13 populations of O. japonicus (L. f. ) Ker-Gawl.and two other species were clustered in unweighted pair group method with arithmetic average (UPGMA) tree. The largest distance was found between O. bockianus Diels, O. bodinieri Lévl.and the populations of O. japonicus (L. f. ) Ker-Gawl.. Among the O. japonicus (L. f. ) Ker-Gawl., the largest distance was found between big leaf type population collected from Sichuan University and the other populations. A high level of genetic variation occurred among the different clime and leaf-type populations. The authors supported that RAPD analysis distinguish O. japonicus (L. f. ) Ker-Gawl. from other species of genus Ophiopogon and identify the population of O.japonicus (L. f. ) Ker-Gawl. used as geo-herbal.