CAJ | 학술논문

目的用异硫氰荧光素(FITC)-右旋糖苷和四甲基若丹明(TMR)-右旋糖苷标记小鼠2-细胞胚胎的两个卵裂球,观察其发育,以探讨囊胚Em-Ab极性的形成.方法向受精卵注射FITC-右旋糖苷确定标记物对胚胎的伤害性,向2-细胞胚胎两个卵裂球分别注射FITC-右旋糖苷和TMR-右旋糖苷,将标记后的胚胎体外培养发育至囊胚,观测两个卵裂球后代在囊胚中的分布情况.结果 2-细胞胚胎两个卵裂球的后代在囊胚中分布并无规律.结论 2-细胞胚胎卵裂球随机分布在囊胚的胚胎部分和胚外部分.
목적 용이류청형광소(FITC)-우선당감화사갑기약단명(TMR)-우선당감표기소서2-세포배태적량개란렬구,관찰기발육,이탐토낭배Em-Ab겁성적형성.방법 향수정란주사FITC-우선당감학정표기물대배태적상해성,향2-세포배태량개란렬구분별주사FITC-우선당감화TMR-우선당감,장표기후적배태체외배양발육지낭배,관측량개란렬구후대재낭배중적분포정황.결과 2-세포배태량개란렬구적후대재낭배중분포병무규률.결론 2-세포배태란렬구수궤분포재낭배적배태부분화배외부분.
Objective Kunming strain(KM) mice were used as animal models. Nontoxic dextran conjugated with tetramethylrhodamine(TMR)and fluorecein isothiocyante(FITC)was microinjected to two of the 2-cell blastomere as molecular probe to trace the development fate of the blastomere ,in order to figure out the mechanisms of the formation of Em-Ab axis. Methods FITC- dextran was injected to zygote in order to make sure if it is noxious. Two blastomeres of 2-cell embryo were injected FITC- dextran and TMR- dextran respectively. Results When labeled embryo develeped to blastocyst, distribution of progeny of 2-cell embryo blastomeres can be detected.Conclusion The cells of blastomere randomly distributed either embryonic parts or extraembryonic parts of blastocyst.