CAJ | 학술논문

用甲基化敏感扩增多态性(MSAP)对背角无齿蚌(Anodonta woodiana)腮、唇瓣、闭壳肌、外套膜和斧足五个组织基因组DNACCGG区域的甲基化水平进行了分析.结果表明,背角无齿蚌腮基因组DNA甲基化比例为47.9%,唇瓣甲基化比例为35.5%,闭壳肌甲基化比例为50%,外套膜甲基化比例为46.3%,斧足甲基化比例为56%;基因组中CCGG区域存在甲基化现象.不同组合甲基化比例不同说明该区域甲基化可能参与到基因的调控中.通过比较不同地点的采集的背角无齿蚌,发现污染严重地区(太湖三山岛水域)采集到的样品和非污染地区(南泉养殖水域)采集的蚌样甲基化区域略有变化.其中的相关性有待进一步的研究.
용갑기화민감확증다태성(MSAP)대배각무치방(Anodonta woodiana)시、진판、폐각기、외투막화부족오개조직기인조DNACCGG구역적갑기화수평진행료분석.결과표명,배각무치방시기인조DNA갑기화비례위47.9%,진판갑기화비례위35.5%,폐각기갑기화비례위50%,외투막갑기화비례위46.3%,부족갑기화비례위56%;기인조중CCGG구역존재갑기화현상.불동조합갑기화비례불동설명해구역갑기화가능삼여도기인적조공중.통과비교불동지점적채집적배각무치방,발현오염엄중지구(태호삼산도수역)채집도적양품화비오염지구(남천양식수역)채집적방양갑기화구역략유변화.기중적상관성유대진일보적연구.
In this study, methylation sensitive amplified polymorphism(MSAP) was applied to analyze the methylation pattern in CCGG sites of the genome in five tissues(gill, labial palp, adductor muscle, mantle and foot) of swan mussels(Anodonta woodiana). The results showed that methylation ratios of the gills, labial palps, adductor muscle, mantle, and foot were 47.9%, 35.5%, 50%, 46.3% and 56%, respectively. Such differences among tissues suggested that methylation may be involved in the regulation of the gene in Anodonta woodiana. The comparison of the swan mussels from different habitats suggested there were some variations in methylated gene loci between those from polluted environment(Sanshandao area of the Taihu Lake) and aquacultural water(Nan-quan aquaculture base of Freshwater Fisheries Research Centre, Chinese Academy ofFishery Sciences). Further studies are needed to investigate the possible mechanism for the aforementioned methylation variations.