缓释骨形态发生蛋白-2听泡内植入对豚鼠内耳功能的影响
완석골형태발생단백-2은포내식입대돈서내이공능적영향
The Affection on Cochlea Function of Guinea-pig by Transplanting Controlled Release BMP-2 into the Middle Ear
  • 万方数据
    听力学及言语疾病杂志 은역학급언어질병잡지
    JOURNAL OF AUDIOLOGY AND SPEECH PATHOLOGY
    2010年 1期 39-42 ,共4页

    李雪盛%孙建军%姜伟%刘肖

    리설성%손건군%강위%류초

    听骨赝复物%骨形态发生蛋白%耳蜗%反应阈 聽骨贗複物%骨形態髮生蛋白%耳蝸%反應閾
    은골안복물%골형태발생단백%이와%반응역
    Prothesis%Bone morphogenetic protein%Cochlea%Hearing threthold
    目的 观察缓释骨形态发生蛋白-2(bone morphogenetic protein-2,BMP-2)植入豚鼠听泡内的成骨诱导情况及对耳蜗功能的影响.方法 制备猪小柱状脱细胞松质骨,与BMP-2复合.选择20只豚鼠,耳后进路进入听泡,左耳植入复合BMP-2的脱细胞松质骨,右耳植入单纯脱细胞松质骨作为对照.分别在植入前、植入即刻、植入后3个月对动物进行ABR测试.并在植入3个月后行组织学及耳蜗基底膜铺片硝酸银染色观察,了解BMP-2诱导成骨情况及对听泡、耳蜗大体结构和毛细胞形态的影响.结果 术后动物健康状况良好.植入后即刻,实验耳及对照耳ABR反应阈略有下降,3个月时恢复正常.植入3个月后,耳蜗及听泡大体形态正常,组织学检查可见含BMP-2脱细胞松质骨孔隙内有新骨形成,未见耳蜗及听泡骨质异常增生,镫骨底板关节无固定;硝酸银染色示毛细胞形态正常,无毛细胞缺失.结论 缓释BMP-2听泡内植入可诱导新生骨形成,但对动物听泡大体形态无影响.不会引起局部骨质异常增生,对动物听功能无影响,有可能应用于听骨链缺损修复重建.染色观察,了解BMP-2诱导成骨情况及对听泡、耳蜗大体结构和毛细胞形态的影响.结果 术后动物健康状况好.植入后即刻,实验耳及对照耳ABR反应阈略有下降,3个月时恢复正常.植入3个月后,耳蜗及听泡大体形态正常,组织学检查可见含BMP-2脱细胞松质骨孔隙内有新骨形成,未见耳蜗及听泡骨质异常增生,镫骨底板关节无固定;硝酸银染色示毛细胞形态正常,无毛细胞缺失.结论 缓释BMP-2听泡内植入可诱导新生骨形成,但对动物听泡大体形态无影响.不会引起局部骨质异常增生,对动物听功能无影响,有可能应用于听骨链缺损修复重建.染色观察,了解BMP-2诱导成骨情况及对听泡、耳蜗大体结构和毛细胞形态的影响.结果 术后动物健康状况好.植入后即刻,实验耳及对照耳ABR反应阈略有下降,3个月时恢复正常.植入3个月后,耳蜗及听泡大
    목적 관찰완석골형태발생단백-2(bone morphogenetic protein-2,BMP-2)식입돈서은포내적성골유도정황급대이와공능적영향.방법 제비저소주상탈세포송질골,여BMP-2복합.선택20지돈서,이후진로진입은포,좌이식입복합BMP-2적탈세포송질골,우이식입단순탈세포송질골작위대조.분별재식입전、식입즉각、식입후3개월대동물진행ABR측시.병재식입3개월후행조직학급이와기저막포편초산은염색관찰,료해BMP-2유도성골정황급대은포、이와대체결구화모세포형태적영향.결과 술후동물건강상황량호.식입후즉각,실험이급대조이ABR반응역략유하강,3개월시회복정상.식입3개월후,이와급은포대체형태정상,조직학검사가견함BMP-2탈세포송질골공극내유신골형성,미견이와급은포골질이상증생,등골저판관절무고정;초산은염색시모세포형태정상,무모세포결실.결론 완석BMP-2은포내식입가유도신생골형성,단대동물은포대체형태무영향.불회인기국부골질이상증생,대동물은공능무영향,유가능응용우은골련결손수복중건.염색관찰,료해BMP-2유도성골정황급대은포、이와대체결구화모세포형태적영향.결과 술후동물건강상황호.식입후즉각,실험이급대조이ABR반응역략유하강,3개월시회복정상.식입3개월후,이와급은포대체형태정상,조직학검사가견함BMP-2탈세포송질골공극내유신골형성,미견이와급은포골질이상증생,등골저판관절무고정;초산은염색시모세포형태정상,무모세포결실.결론 완석BMP-2은포내식입가유도신생골형성,단대동물은포대체형태무영향.불회인기국부골질이상증생,대동물은공능무영향,유가능응용우은골련결손수복중건.염색관찰,료해BMP-2유도성골정황급대은포、이와대체결구화모세포형태적영향.결과 술후동물건강상황호.식입후즉각,실험이급대조이ABR반응역략유하강,3개월시회복정상.식입3개월후,이와급은포대
    Objective The controlled release BMP-2 and implantation into the acoustic bulla of the guineapig and the effects on the cochlea were observed.Methods The acellular cancellous bone was prepared,accompanied with BMP-2.The material accompanied with BMP-2 was implanted into one acoustic bulla of the animal,and the opposite side of the acoustic bulla was implanted with acellular cancellous bone without BMP-2.A total of 20 guinea-pigs were undergone this procedure.The ABR was tested on the animals before and after the operation immediately and 3 month after operation,respectively.3 month after operation,the animals were sacrificed.The osteogenesis induced by BMP-2,the acoustic bulla and the cochlea affected by BMP-2 were observed.The structures of hair cells were observed after being treated with silver nitrate.Results The animals recovered soon after surgery.The hearing thresholds of the animals reduced slightly after the operation,and recovered completely 3 month after.The bulla and the cochlea were normal in shape.The osteogenesis occurred in the pore of the acellular cancellous bone with BMP-2.No abnormal hyperplasia of bone in the hulla and the cochlea was found.The articulation between the stapes and oval window was not merged.The shape of the hair cells was normal and no obvious deletion of the hair cells was noted when compared with control group.Conclusion The controlled release BMP-2 could induce osteogenesis in the bulla of the animals.This material did not affect the shape of the bulla and the hearing thresholds.They did not induce any abnormal hyperplasia of bone in the bulla and might be used to reconstruct the affected ossicles.
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