CAJ | 학술논문

建立高效液相色谱法测定鸢尾苷元磺酸钠(4',5,7-三羟基-6-甲氧基异黄酮-5'-磺酸钠)及其制剂泰克吉宁注射液的含量及有关物质.采用C_(18)色谱柱(150×4.6 mm,5μm),流动相为甲醇-5%醋酸水(80:20),流速为1.0 mL/min,检测波长为263 nm.鸢尾苷元磺酸钠在11～100 μg/mL范围内线性关系良好,回归方程Y=43.3609X-1.5973(r=0.99998),平均回收率为99.77%(n=9).最低检测限为0.052 μg/mL,与有关物质分离良好.本法快速、简便、准确,专属性强.
건립고효액상색보법측정연미감원광산납(4',5,7-삼간기-6-갑양기이황동-5'-광산납)급기제제태극길저주사액적함량급유관물질.채용C_(18)색보주(150×4.6 mm,5μm),류동상위갑순-5%작산수(80:20),류속위1.0 mL/min,검측파장위263 nm.연미감원광산납재11～100 μg/mL범위내선성관계량호,회귀방정Y=43.3609X-1.5973(r=0.99998),평균회수솔위99.77%(n=9).최저검측한위0.052 μg/mL,여유관물질분리량호.본법쾌속、간편、준학,전속성강.
To establish an HPLC method to determine the content and the related substances of teetorigenin sodium sulfo-nite (4' ,5,7-trihydroxy-6-methoxy-7-hydroxymethylisotlavone-5'-sulphoric acid sodium) and its preparation-Taikejining Injection.The HPLC system consisted of C18 column(150 × 4.6 mm,5 μm) ,methanol-5% acetic acid solution(80:20) as mobile phase with UV detection at 263 nm,flow rate: 1.0 mL/min, The linear range was 11-100 μg/mL, r =0.99998 ,the average recovery was 99.77% (n =9).The detection limit was 0.052 μg/mL.The related substances were separated well from tectorigenin sodium sulfonite.This method is rapid, simple, specific and accurate.