CAJ | 학술논문

目的探讨基质金属蛋白酶2(MMP-2)和MMP-9在急性肺血栓栓塞(PTE)中的变化及意义.方法将72只Sprague-Dawley大鼠随机分为假手术组(Sham组),肺血栓栓塞模型组(PTE组)和辛伐他汀干预组(Statins组),每组24只.在造模后2h、6h、24 h分别测定各组大鼠的右心室收缩压和肺动脉平均压.应用明胶酶谱法测定各组大鼠血浆MMP-2和MMP-9的活性.结果 PTE组大鼠各时间点的血浆MMP-9活性明显升高,与Sham组大鼠比较差异有统计学意义(P＜0.05); Statins组大鼠各时间点的血浆MMP-9活性与PTE组大鼠比较明显降低,差异有统计学意义(P＜0.05),但仍高于Sham组大鼠,差异有统计学意义(P＜0.05).PTE组大鼠各时间点的血浆MMP-2活性有升高的趋势,但与Sham组大鼠和Statins组大鼠比较,差异无统计学意义.结论急性PTE可引起血浆MMP-9活性增加,辛伐他汀通过抑制MMP-9的激活而减轻急性PTE所致肺动脉高压.
목적 탐토기질금속단백매2(MMP-2)화MMP-9재급성폐혈전전새(PTE)중적변화급의의.방법 장72지Sprague-Dawley대서수궤분위가수술조(Sham조),폐혈전전새모형조(PTE조)화신벌타정간예조(Statins조),매조24지.재조모후2h、6h、24 h분별측정각조대서적우심실수축압화폐동맥평균압.응용명효매보법측정각조대서혈장MMP-2화MMP-9적활성.결과 PTE조대서각시간점적혈장MMP-9활성명현승고,여Sham조대서비교차이유통계학의의(P＜0.05); Statins조대서각시간점적혈장MMP-9활성여PTE조대서비교명현강저,차이유통계학의의(P＜0.05),단잉고우Sham조대서,차이유통계학의의(P＜0.05).PTE조대서각시간점적혈장MMP-2활성유승고적추세,단여Sham조대서화Statins조대서비교,차이무통계학의의.결론 급성PTE가인기혈장MMP-9활성증가,신벌타정통과억제MMP-9적격활이감경급성PTE소치폐동맥고압.
Objective To explore the change and significance of plasma MMP-2 and MMP-9 activity in acute pulmonary thromboembolism (PTE).Methods 72 male Sprague-Dawley rats were randomly divided into three groups:Sham group,PTE group and Statins group.Right ventricular systolic pressure and mean pulmonary arterial pressure were measured by right heart catheter at different time points following the induction of PTE.The enzymic activity of MMP-2 and MMP-9 in plasma were detected through gelatin zymography.Results Compared with Sham Group,the plasma MMP-9 activity at every time point in PTE Group increased significantly ( P ＜ 0.05).Compared with PTE Group,the plasma MMP-9 activity at every time point in Statins Group decreased significantly ( P ＜0.05),but was still higher than that of Sham Group ( P ＜0.05).No significant difference in MMP-2 activity at every time point was observed among three groups ( P ＞0.05).Conclusions The plasma MMP-9 activity was increased in the PTE rats,pretreatment with simavastatin attenuated acute PTE-induced pulmonary.hypertension through attenuationing of MMP-9 activity.