CAJ | 학술논문

目的研究肠道多形螺旋线虫对T细胞诱导的小鼠结肠炎CD4+T细胞增殖情况的影响.方法用羟基荧光素二醋酸盐琥珀酰亚胺脂(CFSE)染色的卵清蛋白(OVA)特异性CD4+助性T细胞转入重度联合免疫缺陷(SCID)小鼠中,制作小鼠实验性肠炎模型.将实验模型小鼠分为多形螺旋线虫感染组和无感染组(每组n=5),观察多形螺旋线虫感染7d后小鼠结肠炎性反应的组织学变化；以流式细胞仪检测感染3、5、7d小鼠肠系膜淋巴结中CD4+T细胞CFSE的阴性率,判定肠系膜淋巴结中CD4+T细胞的增殖情况.结果与无感染组比较,感染组小鼠第7天时有螺旋线虫感染结肠炎性反应明显加重,黏膜固有层细胞浸润增多,结肠上皮破损增加,病理评分明显升高(5.20±0.84比2.00±0.71,P＜0.05).感染3、5、7d后,感染组小鼠肠系膜淋巴结中CD4+T细胞增殖均比无感染组明显增强,CFSE的阴性率升高[3 d:(7.03±1.61)％比(2.32±0.62)％,5 d:(55.05±13.41)％比(29.10±2.23)％,7d:(76.97±1.89)％比(43.87±5.56)％,均P＜0.05].结论多形螺旋线虫感染在CD4+T细胞诱导的小鼠实验性结肠炎的早期阶段促进了炎性反应的加重,可能与促进CD4+T细胞的增殖有关.
목적 연구장도다형라선선충대T세포유도적소서결장염CD4+T세포증식정황적영향.방법 용간기형광소이작산염호박선아알지(CFSE)염색적란청단백(OVA)특이성CD4+조성T세포전입중도연합면역결함(SCID)소서중,제작소서실험성장염모형.장실험모형소서분위다형라선선충감염조화무감염조(매조n=5),관찰다형라선선충감염7d후소서결장염성반응적조직학변화；이류식세포의검측감염3、5、7d소서장계막림파결중CD4+T세포CFSE적음성솔,판정장계막림파결중CD4+T세포적증식정황.결과 여무감염조비교,감염조소서제7천시유라선선충감염결장염성반응명현가중,점막고유층세포침윤증다,결장상피파손증가,병리평분명현승고(5.20±0.84비2.00±0.71,P＜0.05).감염3、5、7d후,감염조소서장계막림파결중CD4+T세포증식균비무감염조명현증강,CFSE적음성솔승고[3 d:(7.03±1.61)％비(2.32±0.62)％,5 d:(55.05±13.41)％비(29.10±2.23)％,7d:(76.97±1.89)％비(43.87±5.56)％,균P＜0.05].결론 다형라선선충감염재CD4+T세포유도적소서실험성결장염적조기계단촉진료염성반응적가중,가능여촉진CD4+T세포적증식유관.
Objective To determine the effects of Heligmosomoides polygyrus infection on CD4+ T cell proliferation in mice with T cell-induced colitis.Methods The experimental colitis mice model was established via introduction of carboxyfluorescein diaccetate succinimidyl ester (CFSE)-stained ovalbuminspecific CD4+ helper T cell in severe combined immunodeficiency (SCID) mice.The mice were assigned into Heligmosomoides polygyrus infection group (n=5) and Heligmosomoides polygyrus non-infection group (n=5) for determining histological variation in colitis inflammation at day 7.The proportion of negative CFSE-stained CD4+ T cells and the proliferation of all CD4+ T cells at mesenteric lymph nodes were explored at days 3,5 and 7 by using flow cytometry.Results As compared with non- infection group at day 7,Heligmosomoides polygyrus infection led to more serious colitis response.This was associated with increased inflammatory cell infiltration at laminar propria,strengthened epithelial injuries and elevated pathological scores (5.20±0.84 vs 2.00±0.71,P＜0.05).There was a remarkably higher CD4+ T cell proliferation level in mesenteric lymph nodes in Heligmosomoides polygyrus infection group,and elevated proportion of CFSE negative-stained T cell was found at days 3,5 and 7 as compared with non-infection group [day 3:(7.03±1.61)％ vs (2.32±0.62)％; day 5:(55.05±13.41)％ vs (29.10±2.23)％; day 7:(76.97±1.89)％ vs (43.87±5.56)％,all P＜0.05].Conclusion Heligmosomoides polygyrus infection increases CD4+ T cell-induced inflammatory responses in mice with early- stage experimental colitis,which may be associated with the promotion of CD4+ T cell proliferation.