CAJ | 학술논문

目的观察电磁辐射对CD34+造血干细胞JAK2和STAT5蛋白活化的影响,探讨JAK2和STAT5蛋白在电磁辐射致造血干细胞损伤中的作用.方法从脐血中分离培养CD34+造血干细胞,分别用平均功率为1、5、25 mW/cm~2的电磁波辐射20 min,干预组(平均辐射强度为25 mW/cm~2)使用AG490(终浓度10~(-6)mol/L)预处理CD34+造血干细胞30 min.检测正常组、辐射组和干预组12、24和48 h时细胞活力(MTT法)、细胞凋亡率(流式细胞术)、Caspase-3蛋白水平(WB法)、磷酸化JAK2和STAT5蛋白水平(WB法).结果与正常组比较,辐射组细胞12和24 h活力降低、细胞凋亡率升高,具有剂量效应,磷酸化JAK2和STAT5蛋白表达呈升高后降低的趋势.干预组12和24 h细胞活力显著高于辐射25 mW/cm~2组,细胞凋亡率显著低于辐射25 mW/cm~2组,磷酸化JAK2和STAT5蛋白水平较辐射25 mW/cm~2组低.结论 JAK2和STAT5蛋白参与了1-25 mW/cm~2功率范围内的电磁辐射对CD34+造血干细胞的损伤过程,阻断JAK2和STAT5蛋白活化可缓解CD34+造血干细胞凋亡的发生.
목적 관찰전자복사대CD34+조혈간세포JAK2화STAT5단백활화적영향,탐토JAK2화STAT5단백재전자복사치조혈간세포손상중적작용.방법 종제혈중분리배양CD34+조혈간세포,분별용평균공솔위1、5、25 mW/cm~2적전자파복사20 min,간예조(평균복사강도위25 mW/cm~2)사용AG490(종농도10~(-6)mol/L)예처리CD34+조혈간세포30 min.검측정상조、복사조화간예조12、24화48 h시세포활력(MTT법)、세포조망솔(류식세포술)、Caspase-3단백수평(WB법)、린산화JAK2화STAT5단백수평(WB법).결과 여정상조비교,복사조세포12화24 h활력강저、세포조망솔승고,구유제량효응,린산화JAK2화STAT5단백표체정승고후강저적추세.간예조12화24 h세포활력현저고우복사25 mW/cm~2조,세포조망솔현저저우복사25 mW/cm~2조,린산화JAK2화STAT5단백수평교복사25 mW/cm~2조저.결론 JAK2화STAT5단백삼여료1-25 mW/cm~2공솔범위내적전자복사대CD34+조혈간세포적손상과정,조단JAK2화STAT5단백활화가완해CD34+조혈간세포조망적발생.
Objective To observe the effect of electromagnetic radiation on the activation of JAK2 and STAT5 in the CD34 + cord blood stem cell.Methods CD34 + cord blood stem cells were isolated from the umbilical cord blood.Three exposed groups were administered with 1,5,25 mW/cm~2 electromagnetic exposure for 20 minutes,respectively.In the intervention group with the radiation power of 25 mW/cm~2,AG490 of the final concentration of 10~(-6) mol/L was pretreated for 30 minutes.The cell viabilitv (MTT),the rate of apoptosis (FCM),Caspase-3 protein level (WB) and the activation of JAK2 and STAT5 (WB) were assayed.Results Compared with the normal group,the cell viability of the electromagnetic group decreased at 12 h and 24 h,while apoptosis rate increased with dose.The activation of JA K2 and STAT5 increased at 12 h and 24 h and then decreased.The cell viability of the intervention group was significantly higher than the electromagnetic group with 25 mW/cm~2 irradiation at 12 h and 24 h,and apoptosis rate was significantly decreased as activation of JAK2 and STAT5 were lower. Conclusion JAK2 and STAT5 protein are involved in the process of the electromagnetic radiation within a scope of power (1-25 mW/cm~2) damaging CD34 + cord blood stem cells,suggesting that blocking the activation of JA K2 and STAT5 may be effective to alleviate the cell apoptosis of stem cells.