中华生物医学工程杂志
中華生物醫學工程雜誌
중화생물의학공정잡지
CHINESE JOURNAL OF BIOMEDICAL ENGINEERING
2012年
2期
89-92
,共4页
胡志成%朱斌%朱家源%郭栋%陈斌%张凯%胡坤华%黎明涛%唐冰
鬍誌成%硃斌%硃傢源%郭棟%陳斌%張凱%鬍坤華%黎明濤%唐冰
호지성%주빈%주가원%곽동%진빈%장개%호곤화%려명도%당빙
瘢痕%增生%蛋白质组学%电泳,凝胶,双向
瘢痕%增生%蛋白質組學%電泳,凝膠,雙嚮
반흔%증생%단백질조학%전영,응효,쌍향
Cicatrix%Hyperplasia%Proteomics%Electrophoresis,gel,two-dimensional
目的 比较增生性瘢痕与正常皮肤组织的蛋白质组表达差异,筛选出增生性瘢痕的特异蛋白质.方法 以中山大学附属第一医院烧伤外科2010年1月至5月8例增生性瘢痕患者的增生性瘢痕组织和3例整形外科患者正常躯干皮肤为研究对象,运用蛋白质组学技术、双向凝胶电泳对比分析增生性瘢痕组织和正常皮肤组织中蛋白表达差异,选择差异蛋白点进行MALDI - TOF/TOF质谱分析和生物学信息分析.结果 获得重复性较好的双向电泳荧光染色图谱,增生性瘢痕和正常皮肤组织凝胶电泳图谱中平均蛋白点分别为2975和3053,对其中表达差异超过4倍的蛋白点进行质谱分析和数据库检索,共鉴定出24种蛋白质,其中上调蛋白有11种,下调蛋白有13种.结论 成功地建立增生性瘢痕的双向电泳荧光染色图谱,鉴定出增生性瘢痕组织与正常皮肤组织间的差异蛋白质表达.
目的 比較增生性瘢痕與正常皮膚組織的蛋白質組錶達差異,篩選齣增生性瘢痕的特異蛋白質.方法 以中山大學附屬第一醫院燒傷外科2010年1月至5月8例增生性瘢痕患者的增生性瘢痕組織和3例整形外科患者正常軀榦皮膚為研究對象,運用蛋白質組學技術、雙嚮凝膠電泳對比分析增生性瘢痕組織和正常皮膚組織中蛋白錶達差異,選擇差異蛋白點進行MALDI - TOF/TOF質譜分析和生物學信息分析.結果 穫得重複性較好的雙嚮電泳熒光染色圖譜,增生性瘢痕和正常皮膚組織凝膠電泳圖譜中平均蛋白點分彆為2975和3053,對其中錶達差異超過4倍的蛋白點進行質譜分析和數據庫檢索,共鑒定齣24種蛋白質,其中上調蛋白有11種,下調蛋白有13種.結論 成功地建立增生性瘢痕的雙嚮電泳熒光染色圖譜,鑒定齣增生性瘢痕組織與正常皮膚組織間的差異蛋白質錶達.
목적 비교증생성반흔여정상피부조직적단백질조표체차이,사선출증생성반흔적특이단백질.방법 이중산대학부속제일의원소상외과2010년1월지5월8례증생성반흔환자적증생성반흔조직화3례정형외과환자정상구간피부위연구대상,운용단백질조학기술、쌍향응효전영대비분석증생성반흔조직화정상피부조직중단백표체차이,선택차이단백점진행MALDI - TOF/TOF질보분석화생물학신식분석.결과 획득중복성교호적쌍향전영형광염색도보,증생성반흔화정상피부조직응효전영도보중평균단백점분별위2975화3053,대기중표체차이초과4배적단백점진행질보분석화수거고검색,공감정출24충단백질,기중상조단백유11충,하조단백유13충.결론 성공지건립증생성반흔적쌍향전영형광염색도보,감정출증생성반흔조직여정상피부조직간적차이단백질표체.
Objective To screen specific proteins in patients with hypertrophic scar via comparing the distinction in proteomics to normal skin.Methods Hypertrophic scar tissues (8 patients with hypertrophic scar)and normal cutaneous tissues (3 patients underwent trunk plastic surgery) were collected from Department of Burns Surgery,The First Affiliated Hospital of Sun Yat-sen University,between January 2010 and May 2010.The distinction in proteomics between hypertrophic scars and normal cutaneous tissues was compared via twodimensional gel electrophoresis.The protein spots with differential expression were subjected to MALDI-TOF/TOF mass spectrometry and bio-information analysis.Results Two-dimensional electrophoresis fluorescent staining atlas with high repeatability was obtained,showing an average of 2975 protein spots in hypertrophic scar and 3053 in normal cutaneous tissue.Further analyses on the spots with a minimum of 4-fold distinction identified 24proteins ( 11 up-regulated and 13 down-regulated) via mass spectrometry and database searching.Conclusion Successful establishment of two-dimensional electrophoresis fluorescent staining atlas is helpful to identification of differential proteins between hypertrophic scar and normal skin.
