CAJ | 학술논문

目的通过反复加减压建立小型猪减压性骨坏死模型并观察其血液流变学改变.方法 12头小型香猪,每周2～3次暴露于0.45 MPa加压舱内,每次6 h,1 min匀速减至常压.用锥板式黏度计测定全血黏度,光镜下血小板计数,反复加减压3个月后进行股骨同位素发射计算机扫描(ECT)及组织病理学检查.结果 ECT检查发现,动物股骨头同位素聚集增加,骨病理检查发现股骨发生缺血性坏死改变,血小板计数随加压次数增多而进行性降低,低剪切率下全血黏度则进行性升高.结论减压性气泡引起的血小板消耗、血黏度增高是骨缺血坏死的原因之一.
목적 통과반복가감압건립소형저감압성골배사모형병관찰기혈액류변학개변.방법 12두소형향저,매주2～3차폭로우0.45 MPa가압창내,매차6 h,1 min균속감지상압.용추판식점도계측정전혈점도,광경하혈소판계수,반복가감압3개월후진행고골동위소발사계산궤소묘(ECT)급조직병이학검사.결과 ECT검사발현,동물고골두동위소취집증가,골병리검사발현고골발생결혈성배사개변,혈소판계수수가압차수증다이진행성강저,저전절솔하전혈점도칙진행성승고.결론 감압성기포인기적혈소판소모、혈점도증고시골결혈배사적원인지일.
Objective To develop the dysharie osteonecrosis (DON) model in mini pigs induced by rapid decompression, following repeated hyperbaric exposures and observe changes in blood rheology in DON. Methods Twelve male mini pigs were exposed to compressed air of 0.45 MPa for 6 hours twice to three times a week and were then brought to the surface with linear decompression at a rate of 0.35 MPa/min. Blood viscosity was measured with cone-plate type viscosimeter, platelet count was made under the light microscope. Emission of computed tomography (ECT) and pathologic examination were performed for the evaluation of accumulated rate of 99Tc in the femur, following 3 months of repeated compression and decompression. Results ECT showed an increased accumulation of isotope in the femur, when a comparison was made between the experimental animals and the control. Osteopatholngy revealed changes of osteonecrosis in the femur. Platelet count decreased progressively with frequencies of pressure exposures, and meanwhile blood viscosity also increased progressively at a low shear rate. Conclusions Dysharie osteronecrosis was usually accompanied with bone ischemia. Platelet consumption and increased blood viscosity induced by decompression bubbles could be attributed to decreased blood flow in the bone, resulting in osteonecrosis.