中华眼科杂志
中華眼科雜誌
중화안과잡지
Chinese Journal of Ophthalmology
2011年
3期
248-251
,共4页
李满%吴晓梅%李甘地%夏瑞南
李滿%吳曉梅%李甘地%夏瑞南
리만%오효매%리감지%하서남
睑板腺%眼睑肿瘤%疾病模型,动物%肿瘤移植%小鼠,裸
瞼闆腺%眼瞼腫瘤%疾病模型,動物%腫瘤移植%小鼠,裸
검판선%안검종류%질병모형,동물%종류이식%소서,라
Meibomian glands%Eyelid neoplasms%Disease models,animal%Neoplasm transplantation%Mice,nude
目的 探讨建立睑板腺癌裸鼠移植瘤模型的方法,研究肿瘤生物学特性.方法 采用组织块法,将4例新鲜人睑板腺癌组织块直接接种于8只裸鼠皮下.观察成瘤时间、肿瘤生长情况.待移植瘤体成活并生长至一定大小后,取部分瘤体进行HE染色,苏丹Ⅲ染色以及广谱细胞角蛋白、上皮膜抗原免疫组织化学染色.结果 新鲜人睑板腺癌组织能在裸鼠皮下存活并生长,原代移植瘤第5周开始生长,第10周加速生长.HE染色光镜观察,肿瘤呈片状或团状排列,多数细胞呈睑板腺细胞样分化,瘤细胞比较大,呈多边形,胞质呈泡沫状,细胞核位于中央稍向细胞周边偏位,核异型性明显,核分裂象易见.苏丹Ⅲ染色,细胞质和细胞间隙内可见橙红色脂肪颗粒.免疫组织化学染色,广谱细胞角蛋白、上皮膜抗原均为阳性表达.结论 采用组织块法成功建立了人睑板腺癌的裸鼠皮下移植瘤模型.该移植瘤良好地保留了人睑板腺癌的生物学特性.
目的 探討建立瞼闆腺癌裸鼠移植瘤模型的方法,研究腫瘤生物學特性.方法 採用組織塊法,將4例新鮮人瞼闆腺癌組織塊直接接種于8隻裸鼠皮下.觀察成瘤時間、腫瘤生長情況.待移植瘤體成活併生長至一定大小後,取部分瘤體進行HE染色,囌丹Ⅲ染色以及廣譜細胞角蛋白、上皮膜抗原免疫組織化學染色.結果 新鮮人瞼闆腺癌組織能在裸鼠皮下存活併生長,原代移植瘤第5週開始生長,第10週加速生長.HE染色光鏡觀察,腫瘤呈片狀或糰狀排列,多數細胞呈瞼闆腺細胞樣分化,瘤細胞比較大,呈多邊形,胞質呈泡沫狀,細胞覈位于中央稍嚮細胞週邊偏位,覈異型性明顯,覈分裂象易見.囌丹Ⅲ染色,細胞質和細胞間隙內可見橙紅色脂肪顆粒.免疫組織化學染色,廣譜細胞角蛋白、上皮膜抗原均為暘性錶達.結論 採用組織塊法成功建立瞭人瞼闆腺癌的裸鼠皮下移植瘤模型.該移植瘤良好地保留瞭人瞼闆腺癌的生物學特性.
목적 탐토건립검판선암라서이식류모형적방법,연구종류생물학특성.방법 채용조직괴법,장4례신선인검판선암조직괴직접접충우8지라서피하.관찰성류시간、종류생장정황.대이식류체성활병생장지일정대소후,취부분류체진행HE염색,소단Ⅲ염색이급엄보세포각단백、상피막항원면역조직화학염색.결과 신선인검판선암조직능재라서피하존활병생장,원대이식류제5주개시생장,제10주가속생장.HE염색광경관찰,종류정편상혹단상배렬,다수세포정검판선세포양분화,류세포비교대,정다변형,포질정포말상,세포핵위우중앙초향세포주변편위,핵이형성명현,핵분렬상역견.소단Ⅲ염색,세포질화세포간극내가견등홍색지방과립.면역조직화학염색,엄보세포각단백、상피막항원균위양성표체.결론 채용조직괴법성공건립료인검판선암적라서피하이식류모형.해이식류량호지보류료인검판선암적생물학특성.
Objective To explore the methods to establish a xenografted model of mebomian gland carcinoma in nude mice, then find the best method to establish the animal model. The last purpose of this study was to establish a suitable animal model for clinic therapy and basic research of mebomian gland carcinoma. Methods Small pieces of mebomian gland carcinoma tissue from four patients were transplanted subcutaneously in the flanks of eight nude mice through a skin incision. Nature of transplantation tumor including formation, growth and size were observed. The transplanted tumors were stained with hematoxylineosin,Sudan Ⅲ and Immunohistochemistry. Results The human mebomian gland carcinoma tissue can survived in nude mice. The pathologic examination revealed that the transplanted tumor was arranged in mass. The tumor cells were round or multisided. The proportion of the nuclei and cytoplasm was imbalance.Nuclear division could be easily observed. The test of Sudan Ⅲ was positive. Immunohistochemical observation: the results showed that both CK and EMA were positive. Conclusions The subcutaneously xenotransplanted tumor model of human mebomian gland carcinoma with tissue explant in nude mice was successfully established. The transplanted tumor maintained the properties of human mebomian gland carcinoma.
