中华实验外科杂志
中華實驗外科雜誌
중화실험외과잡지
CHINESE JOURNAL OF EXPERIMENTAL SURGERY
2012年
5期
854-856
,共3页
刘三光%吕海涛%穆宏凌%闫长青%刘建华
劉三光%呂海濤%穆宏凌%閆長青%劉建華
류삼광%려해도%목굉릉%염장청%류건화
肝癌%免疫结核球蛋白%血管内皮生长因子%逆转录-聚合酶链反应%免疫印迹实验
肝癌%免疫結覈毬蛋白%血管內皮生長因子%逆轉錄-聚閤酶鏈反應%免疫印跡實驗
간암%면역결핵구단백%혈관내피생장인자%역전록-취합매련반응%면역인적실험
Hepatoma%Immunoglobulin-binding protein%Vascular endothelial growth factor-C%Reverse transcription-polymerase chain reaction%Western blotting
目的 检测免疫结核球蛋白(Bip)、血管内皮生长因子(VEGF)-C在肝癌组织中的表达,探讨其在肝癌生长中的作用机制.方法 采用逆转录-聚合酶链反应(RT-PCR)检测术中所取新鲜的42例肝癌标本、15例同个体癌旁1.0 cm肝组织和15例正常肝组织中Bip、VEGF-C的mRNA表达,应用Western blot方法验证57例肝癌组织和正常肝组织标本中Bip及VEGF-C在蛋白水平的表达.结果 肝癌组织、癌旁肝组织、正常肝组织中Bip mRNA相对表达量分别为0.4772±0.0401、0.4332±0.0488、0.4301±0.0398,Bip mRNA在3组之间的表达呈下降趋势(P<0.05).肝癌组织、癌旁肝组织、正常肝组织中VEGF-C mRNA相对表达量分别为0.4447±0.0335、0.4195±0.0334、0.4019±0.0259,VEGF-C mRNA在3组之间的表达呈下降趋势(P<0.05);实验对肝癌组织和正常肝组织中Bip和VEGF-C的蛋白表达进行定性验证:在肝癌组织和正常肝组织中均有两种蛋白的表达,但肝癌组织中的表达明显高于正常肝组织.结论 在肝癌的生长过程中存在未折叠蛋白反应(UPR)的激活.在肝癌组织中Bip、VEGF-C mRNA和蛋白的表达一致,均呈高表达.这些基因和蛋白表达的改变可能与肝癌的发生、生长及转移密切相关.
目的 檢測免疫結覈毬蛋白(Bip)、血管內皮生長因子(VEGF)-C在肝癌組織中的錶達,探討其在肝癌生長中的作用機製.方法 採用逆轉錄-聚閤酶鏈反應(RT-PCR)檢測術中所取新鮮的42例肝癌標本、15例同箇體癌徬1.0 cm肝組織和15例正常肝組織中Bip、VEGF-C的mRNA錶達,應用Western blot方法驗證57例肝癌組織和正常肝組織標本中Bip及VEGF-C在蛋白水平的錶達.結果 肝癌組織、癌徬肝組織、正常肝組織中Bip mRNA相對錶達量分彆為0.4772±0.0401、0.4332±0.0488、0.4301±0.0398,Bip mRNA在3組之間的錶達呈下降趨勢(P<0.05).肝癌組織、癌徬肝組織、正常肝組織中VEGF-C mRNA相對錶達量分彆為0.4447±0.0335、0.4195±0.0334、0.4019±0.0259,VEGF-C mRNA在3組之間的錶達呈下降趨勢(P<0.05);實驗對肝癌組織和正常肝組織中Bip和VEGF-C的蛋白錶達進行定性驗證:在肝癌組織和正常肝組織中均有兩種蛋白的錶達,但肝癌組織中的錶達明顯高于正常肝組織.結論 在肝癌的生長過程中存在未摺疊蛋白反應(UPR)的激活.在肝癌組織中Bip、VEGF-C mRNA和蛋白的錶達一緻,均呈高錶達.這些基因和蛋白錶達的改變可能與肝癌的髮生、生長及轉移密切相關.
목적 검측면역결핵구단백(Bip)、혈관내피생장인자(VEGF)-C재간암조직중적표체,탐토기재간암생장중적작용궤제.방법 채용역전록-취합매련반응(RT-PCR)검측술중소취신선적42례간암표본、15례동개체암방1.0 cm간조직화15례정상간조직중Bip、VEGF-C적mRNA표체,응용Western blot방법험증57례간암조직화정상간조직표본중Bip급VEGF-C재단백수평적표체.결과 간암조직、암방간조직、정상간조직중Bip mRNA상대표체량분별위0.4772±0.0401、0.4332±0.0488、0.4301±0.0398,Bip mRNA재3조지간적표체정하강추세(P<0.05).간암조직、암방간조직、정상간조직중VEGF-C mRNA상대표체량분별위0.4447±0.0335、0.4195±0.0334、0.4019±0.0259,VEGF-C mRNA재3조지간적표체정하강추세(P<0.05);실험대간암조직화정상간조직중Bip화VEGF-C적단백표체진행정성험증:재간암조직화정상간조직중균유량충단백적표체,단간암조직중적표체명현고우정상간조직.결론 재간암적생장과정중존재미절첩단백반응(UPR)적격활.재간암조직중Bip、VEGF-C mRNA화단백적표체일치,균정고표체.저사기인화단백표체적개변가능여간암적발생、생장급전이밀절상관.
Objective To detect the express of immunoglobulin-binding protein (Bip),vascular endothelial growth factor (VEGF)-C in the tissue of hepatoma;demonstrating that it exist activation of unfold protein response (UPR) in process of growth,and the expression of UPR was higher than normal tissue;approaching the mechanism of action in the growth of hepatoma,searching the new target for the therapy of hepatoma.Methods To study the mRNA of Bip,VEGF-C in 42 case example of hepatome,15 case paired adjacent hepatic tissues to carcinoma and 15 case normal hepatic tissues by reverse transcription-polymerase chain reaction (RT-PCR).To qualitation check the expression of Bip and VEGF-C in the level of protein by Western blotting.Results The express of Bip mRNA in hepatoma tissue,adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0.4772 ± 0.0401,0.4332 ± 0.0488,0.4301 ± 0.0398 ; the express of Bip mRNA was higher in hepatoma than others ( P < 0.05 ).The express of VEGF-C mRNA in hepatoma tissue,adjacent hepatic tissue to carcinoma and normal hepatic tissue respectively was 0.4447 ± 0.0335,0.4195 ± 0.0334,0.4019 ± 0.0259.The express of VEGF-C mRNA was higher in hepatoma than others ( P <0.05 ).The experiment indicate that the two protein all express in the hepatoma tissue and normal hepatic tissue,but they were higher in hepatoma tissue than in the normal hepatic tissue.Conclusion It exist activation of UPR in process of growth.The express of Bip,VEGF-C protern and their mRNA was accordant,all of them were higher in hepatoma tissue than in normal hepatic tissue.The changes of these gene and protern is possible to have the relationship with the carcinogenesis,growth and transfer of hepatoma.And it may have significant effect in molecule mechanism of heoatoma.