中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2010年
23期
1645-1647
,共3页
脑%大鼠%再灌注%凋亡
腦%大鼠%再灌註%凋亡
뇌%대서%재관주%조망
Brain%Rats%Reperfusion%Apoptosis
目的 探讨醒脑静与血塞通注射液联合应用对大鼠脑缺血再灌注损伤保护作用的机制.方法 雄性SD大鼠152只,采用Langa线栓法制备大鼠大脑中动脉缺血再灌注模型.分为假手术组、醒脑静组、醒脑静血塞通联合应用组和对照组.分别于缺血再灌注2、4、8、24、48、72 h时,取脑组织,用于脑组织匀浆液的超氧化物歧化酶(SOD)、丙二醛(MDA)检测,并进行凋亡染色.统计学处理:成组设计资料的t检验.结果 醒脑静组SOD和MDA的变化与对照组相比减弱(P<0.05);醒脑静血塞通联合应用组的这两项指标的变化进一步减弱(P<0.05).醒脑静组较相应时相点对照组凋亡细胞减少(P<0.05).而醒脑静血塞通联合应用组的凋亡细胞较醒脑静组进一步减少(4、8 h:P<0.05,24、48、72 h:P<0.01).结论 醒脑静、血塞通注射液联合应用对于脑缺血再灌注损伤的保护有协同作用,可延缓氧自由基的产生,减少神经细胞凋亡.
目的 探討醒腦靜與血塞通註射液聯閤應用對大鼠腦缺血再灌註損傷保護作用的機製.方法 雄性SD大鼠152隻,採用Langa線栓法製備大鼠大腦中動脈缺血再灌註模型.分為假手術組、醒腦靜組、醒腦靜血塞通聯閤應用組和對照組.分彆于缺血再灌註2、4、8、24、48、72 h時,取腦組織,用于腦組織勻漿液的超氧化物歧化酶(SOD)、丙二醛(MDA)檢測,併進行凋亡染色.統計學處理:成組設計資料的t檢驗.結果 醒腦靜組SOD和MDA的變化與對照組相比減弱(P<0.05);醒腦靜血塞通聯閤應用組的這兩項指標的變化進一步減弱(P<0.05).醒腦靜組較相應時相點對照組凋亡細胞減少(P<0.05).而醒腦靜血塞通聯閤應用組的凋亡細胞較醒腦靜組進一步減少(4、8 h:P<0.05,24、48、72 h:P<0.01).結論 醒腦靜、血塞通註射液聯閤應用對于腦缺血再灌註損傷的保護有協同作用,可延緩氧自由基的產生,減少神經細胞凋亡.
목적 탐토성뇌정여혈새통주사액연합응용대대서뇌결혈재관주손상보호작용적궤제.방법 웅성SD대서152지,채용Langa선전법제비대서대뇌중동맥결혈재관주모형.분위가수술조、성뇌정조、성뇌정혈새통연합응용조화대조조.분별우결혈재관주2、4、8、24、48、72 h시,취뇌조직,용우뇌조직균장액적초양화물기화매(SOD)、병이철(MDA)검측,병진행조망염색.통계학처리:성조설계자료적t검험.결과 성뇌정조SOD화MDA적변화여대조조상비감약(P<0.05);성뇌정혈새통연합응용조적저량항지표적변화진일보감약(P<0.05).성뇌정조교상응시상점대조조조망세포감소(P<0.05).이성뇌정혈새통연합응용조적조망세포교성뇌정조진일보감소(4、8 h:P<0.05,24、48、72 h:P<0.01).결론 성뇌정、혈새통주사액연합응용대우뇌결혈재관주손상적보호유협동작용,가연완양자유기적산생,감소신경세포조망.
Objective To investigate the mechanism of protective effect of Xingnaojing and Xuesaitong injections on cerebral ischemic reperfusion injury in rats.Methods The focal cerebral ischemia & reperfusion model was established by middle cerebral artery occlusion(MCAO).A total of 152 male SD rats were randomly assigned into 19 groups:sham operated group,Xingnaojing group,Xingnaojing plus Xuesaitong group and control group according to different reperfusion durations:2,4,8,24,48,72 h.the meantime,rats in control group received the same ip dose of saline.The levels of SOD and MDA were detected.The number of apoptotic neurons was detected by terminal-deoxynucleotidyl transferase medicated nick end labeling(TUNEL).Results During ischemic reperfusion,the MDA content of brain homogenate increased while the SOD activity decreased(P<0.05).Xingnaojing could significantly inhibit the increase of MDA after cerebral ischemic reperfusion(P<0.05)and the decrease of SOD activity in rats.The changes of SOD and MDA were smaller in the Xingnaojing plus Xuesaitong group than those in the Xinnaojing group (P<0.01).The number of apoptotic neurons in the Xingnaojing group was significantly lower than that in control group(P<0.05).And the number of apoptotic neurons in the Xingnaojing plus Xuesaitong group was even lower than that in the Xingnaojing group(4、8 h:P<0.05,24,48、72 h:P<0.01).Conclusion The Xingnaojing plus Xuesaitong injection has protective function after cerebral ischemic reperfusion.
