CAJ | 학술논문

目的探讨EZH2基因在子宫颈鳞状细胞癌(简称:鳞癌)组织中的表达及其临床意义.方法采用反转录-聚合酶链反应( RT-PCR)方法检测21例正常子宫颈组织、27例子宫颈上皮内瘤变( CIN)及48例子宫颈鳞癌组织中EZH2 mRNA的表达,并分析EZH2与各临床病理参数之间的关系.结果 EZH2 mRNA在子宫颈鳞癌中的表达为(1.67±0.01),明显高于正常子宫颈组织(0)和CIN组织(0.36±0.02)(均P＜ 0.01);EZH2mRNA在子宫颈鳞癌中表达与患者年龄无明显相关性(P＞0.05),而与组织学分期、临床病理分期、浸润深度及淋巴结转移有显著相关性(均P＜ 0.01).结论 EZH2在子宫颈鳞癌中高表达,提示其在子宫颈鳞癌发生、发展过程中可能起重要作用,有可能成为子宫颈鳞癌一种新的肿瘤标志物.
목적 탐토EZH2기인재자궁경린상세포암(간칭:린암)조직중적표체급기림상의의.방법 채용반전록-취합매련반응( RT-PCR)방법검측21례정상자궁경조직、27례자궁경상피내류변( CIN)급48례자궁경린암조직중EZH2 mRNA적표체,병분석EZH2여각림상병리삼수지간적관계.결과 EZH2 mRNA재자궁경린암중적표체위(1.67±0.01),명현고우정상자궁경조직(0)화CIN조직(0.36±0.02)(균P＜ 0.01);EZH2mRNA재자궁경린암중표체여환자년령무명현상관성(P＞0.05),이여조직학분기、림상병리분기、침윤심도급림파결전이유현저상관성(균P＜ 0.01).결론 EZH2재자궁경린암중고표체,제시기재자궁경린암발생、발전과정중가능기중요작용,유가능성위자궁경린암일충신적종류표지물.
Objective To study the expression of EZH2 gene in cervical squamous carcinoma and its clinicopathologic significance. Methods The expression of EZH2 mRNA was detected in 21 samples of normal cervical tissue, 27 samples of CIN tissue and 48 samples of cervical squamous carcinoma tissue by RT-PCR. And the relationship between EZH2 expression and the clinical pathological characteristic was analyzed. Results The level of EZH2 mRNA in cervical squamous carcinoma tissues (1.67±0.01) were significantly higher than that in the normal cervical tissues (0) and CIN tissues (0.36±0.02) (P ＜ 0.01).There was no correlation between EZH2 and ages (P ＞ 0.05), while the expression of EZH2 was highly correlated with histologic stage,clinical stage,muscular invasion depth and lymph node metastasis of cervical squamous carcinoma (P ＜ 0.01).Conclusion The over-expression of the EZH2mRNA may play an important role in the pathogenesis and progression of cervical squamous carcinoma, suggesting that EZH2 might be a new biomarker for diagnosing cervical squamous carcinoma.