CAJ | 학술논문

目的:研究瘦素对大鼠背根神经节(DRG)神经元中Bcl-2和Bax蛋白表达的影响.方法:用不同浓度(1、 10和100nmol/L)的瘦素处理体外培养大鼠DRG神经元;采用免疫印迹技术,检测神经元中Bcl-2和Bax蛋白的表达变化.结果:10和100nmol/L的瘦素能促进DRG神经元中Bcl-2蛋白的表达;瘦素对Bax蛋白表达没有影响.结论:瘦素能促进DRG神经元中Bcl-2蛋白的表达,提示瘦素可通过提高Bcl-2蛋白表达来抑制Bax的促凋亡作用,从而改善DRG神经元的存活.这对进一步研究瘦素对DRG功能调节具有重要的意义.
목적:연구수소대대서배근신경절(DRG)신경원중Bcl-2화Bax단백표체적영향.방법:용불동농도(1、 10화100nmol/L)적수소처리체외배양대서DRG신경원;채용면역인적기술,검측신경원중Bcl-2화Bax단백적표체변화.결과:10화100nmol/L적수소능촉진DRG신경원중Bcl-2단백적표체;수소대Bax단백표체몰유영향.결론:수소능촉진DRG신경원중Bcl-2단백적표체,제시수소가통과제고Bcl-2단백표체래억제Bax적촉조망작용,종이개선DRG신경원적존활.저대진일보연구수소대DRG공능조절구유중요적의의.
Objective:To investigate the effects of leptin on the expression of Bcl-2 and Bax proteins in cultured neurons of dorsal root ganglion (DRG). Methods:Different concentrations of leptin (1, 10 and 100 nmol/L) were used to treat the cultured DRG neurons. Western blot was used to detect the expression of Bcl-2 and Bax proteins in DRG neurons. Results:10 and 100 nmol/L leptin could increase the expression of Bcl-2 protein in cultured DRG neurons; However, it could not influence the expression of Bax protein. Conclusion:Leptin can suppress apoptosis of cultured rat DRG neurons through upregu-lating Bcl-2 expression but inhibiting Bax expression to improve survival of neurons, which should be important to the further research on its physiological function of the DRG.