国际眼科杂志
國際眼科雜誌
국제안과잡지
INTERNATIONAL JOURNAL OF OPHTHALMOLOGY
2010年
1期
5-8
,共4页
王方%彭清华%姚小磊%吴权龙%李点
王方%彭清華%姚小磊%吳權龍%李點
왕방%팽청화%요소뢰%오권룡%리점
干眼症%泪腺上皮细胞%培养%STAT1的磷酸化蛋白%雄激素受体%氟他胺
榦眼癥%淚腺上皮細胞%培養%STAT1的燐痠化蛋白%雄激素受體%氟他胺
간안증%루선상피세포%배양%STAT1적린산화단백%웅격소수체%불타알
dry eye%lacrimal gland epithelial cells%culture%STAT1 phosphoprotein%androgen receptor%flutamide
目的:探讨密蒙花总黄酮含药血浆和雄激素受体阻滞剂对泪腺上皮细胞中STAT1的磷酸化蛋白表达的影响.方法:体外分离及培养泪腺上皮细胞.以H2O2诱导大鼠泪腺上皮细胞凋亡,建立雄激素水平下降所致干眼症的细胞凋亡状态.设立空白血浆组、密蒙花总黄酮含药血浆干预组、丙酸睾酮干预组,分别观察各组STAT1的磷酸化蛋白表达情况;并应用雄激素受体阻滞剂氟他胺,考察密蒙花总黄酮的拟雄激素效应.结果:免疫印迹结果表明,含药血浆干预后,密蒙花总黄酮含药血浆干预组中(0.353±0.494)与丙酸睾酮干预组中STAT1的磷酸化蛋白(0.502±0.036)的表达增强,两组间的差异有统计学意义(P<0.01).各组加入雄激素受体阻滞剂后,各组间(分别是0.268±0.061,0.283±0.106,0.213±0.071)的STAT1的磷酸化蛋白表达间无差异.结论:密蒙花总黄酮含药血浆可促进STAT1的磷酸化表达,并激活STAT1细胞信号传导通路,而产生与丙酸睾酮相同的雄激素效应.
目的:探討密矇花總黃酮含藥血漿和雄激素受體阻滯劑對淚腺上皮細胞中STAT1的燐痠化蛋白錶達的影響.方法:體外分離及培養淚腺上皮細胞.以H2O2誘導大鼠淚腺上皮細胞凋亡,建立雄激素水平下降所緻榦眼癥的細胞凋亡狀態.設立空白血漿組、密矇花總黃酮含藥血漿榦預組、丙痠睪酮榦預組,分彆觀察各組STAT1的燐痠化蛋白錶達情況;併應用雄激素受體阻滯劑氟他胺,攷察密矇花總黃酮的擬雄激素效應.結果:免疫印跡結果錶明,含藥血漿榦預後,密矇花總黃酮含藥血漿榦預組中(0.353±0.494)與丙痠睪酮榦預組中STAT1的燐痠化蛋白(0.502±0.036)的錶達增彊,兩組間的差異有統計學意義(P<0.01).各組加入雄激素受體阻滯劑後,各組間(分彆是0.268±0.061,0.283±0.106,0.213±0.071)的STAT1的燐痠化蛋白錶達間無差異.結論:密矇花總黃酮含藥血漿可促進STAT1的燐痠化錶達,併激活STAT1細胞信號傳導通路,而產生與丙痠睪酮相同的雄激素效應.
목적:탐토밀몽화총황동함약혈장화웅격소수체조체제대루선상피세포중STAT1적린산화단백표체적영향.방법:체외분리급배양루선상피세포.이H2O2유도대서루선상피세포조망,건립웅격소수평하강소치간안증적세포조망상태.설립공백혈장조、밀몽화총황동함약혈장간예조、병산고동간예조,분별관찰각조STAT1적린산화단백표체정황;병응용웅격소수체조체제불타알,고찰밀몽화총황동적의웅격소효응.결과:면역인적결과표명,함약혈장간예후,밀몽화총황동함약혈장간예조중(0.353±0.494)여병산고동간예조중STAT1적린산화단백(0.502±0.036)적표체증강,량조간적차이유통계학의의(P<0.01).각조가입웅격소수체조체제후,각조간(분별시0.268±0.061,0.283±0.106,0.213±0.071)적STAT1적린산화단백표체간무차이.결론:밀몽화총황동함약혈장가촉진STAT1적린산화표체,병격활STAT1세포신호전도통로,이산생여병산고동상동적웅격소효응.
AIM: To explore the effect of Buddleia flavonoids drug-containing plasma and androgen receptor(AR) blocker on the expression of STAT1 phosphoprotein.METHODS: In vitro lacrimal gland epithelial cells were cultivated with H2O2 to establish the dry eye apoptosis state. Blank plasma group, Buddleia officinalis plasma total flavonoids interfere with drug-containing group, and the intervention group of testosterone propionate were set. The expressions of STAT1 phosphoprotein of each group were observed by Western blot and AR blocker flutamide was used to explore the intended androgen effect of Buddleia flavonoids.RESULTS: After the intervention of drug-containing plasma, the expression of STAT1 Phosphoprotein in Buddleja officinalis drug-containing plasma intervention group(0.353±0.494) and testosterone propionate intervention group(0.502±0.036) were enhanced and the differences between the two groups were significant (P<0.01). After using the AR blocker in all groups, the expression of STAT1 phosphoprotein in each group (0.268±0.061,0.283±0.106,0.213±0.071) had no difference.CONCLUSION: Buddleja officinalis drug-containing plasma total flavonoids can promote the expression of STAT1 phosphorylation.
