CAJ | 학술논문

目的:观察银杏内酯B对体外培养的大鼠视网膜神经细胞内钙离子浓度和线粒体功能的影响.方法:采用体外原代培养的大鼠视网膜神经细胞,建立谷氨酸损伤的视网膜神经细胞凋亡模型,与银杏内酯B共同培养,用激光扫描共聚焦显微镜检测对视网膜神经细胞内钙离子浓度和线粒体膜电位的影响.结果:谷氨酸(8 mmol/L)作用后,视网膜神经细胞存活率降低,细胞凋亡增加,细胞内钙离子浓度增加,线粒体膜电位下降.GB干预后,钙离子浓度降低,线粒体膜电位显著升高,细胞凋亡明显减少.结论:GB能对抗谷氨酸兴奋性毒性, 保护视网膜神经细胞,这一作用可能是通过降低细胞内钙离子浓度和升高线粒体膜电位来实现的.
목적:관찰은행내지B대체외배양적대서시망막신경세포내개리자농도화선립체공능적영향.방법:채용체외원대배양적대서시망막신경세포,건립곡안산손상적시망막신경세포조망모형,여은행내지B공동배양,용격광소묘공취초현미경검측대시망막신경세포내개리자농도화선립체막전위적영향.결과:곡안산(8 mmol/L)작용후,시망막신경세포존활솔강저,세포조망증가,세포내개리자농도증가,선립체막전위하강.GB간예후,개리자농도강저,선립체막전위현저승고,세포조망명현감소.결론:GB능대항곡안산흥강성독성, 보호시망막신경세포,저일작용가능시통과강저세포내개리자농도화승고선립체막전위래실현적.
AIM: To observe the effect of ginkgolide B (CB) on the intracellular calcium ion concentration ( [ Ca~(2+) ]_i) and mitochondrial function of cultured rat retinal neurons in vitro. METHODS: in vitro primary culture of rat retinal neurons was used in the experiment. The apoptosis model of glutamate - induced retinal neurons was established and co - cultured with ginkgolide B. The [ Ca~(2+) ]_i and mitochondrial membrane potential of the retinal neurons were detected by laser scanning confocal microscope. RESULTS: Glutamate decreased the survival rate of retinal neurons, increased the apoptosis and the [ Ca~(2+) ]_i, lowered the mitochondrial membrane potential. The [ Ca~(2+) ]_i was clearly diminished and the mitochondrial membrane potential was significantly increased with the GB intervention, and the apoptosis decreased significantly. CONCLUSION: GB protects retinal neurons from glutamate induced neurotoxicity. The effect of GB on retinal neurons might be due to its ability to decrease the [Ca~(2+) ]_i and increase mitochondrial membrane potential.