CAJ | 학술논문

黄秋葵是近几年来从日本和我国台湾引进的一种蔬菜作物.近期,广东的黄秋葵上发生了黄脉曲叶病.病株的典型症状表现为叶脉黄化,在叶片正面形成网络状,在叶背面叶脉肿大突起明显,病株幼叶小且向下卷曲,甚至整片幼叶黄化.植株早期被感染表现矮化.在发生黄脉曲叶病的黄秋葵田阃,其病株率高达60%以上.用烟粉虱传双生病毒简并引物对随机采集的病样进行PCR检测,从这些病样中均能扩增出1条预期大小为570 bp的特异片段;基因克隆及测序分析结果表明,与该特异片段同源的均属双生病毒科菜豆金色花叶病毒属病毒DNA,其中与木尔坦棉花曲叶病毒(Cotton leaf curl Muhan virus,CLCuMV)分离物G6相似性最高,为99%.这些研究结果表明,广东黄秋葵黄脉曲叶病中存在烟粉虱传双生病毒,该病害可能也是由CLCuMV侵染引起的.
황추규시근궤년래종일본화아국태만인진적일충소채작물.근기,엄동적황추규상발생료황맥곡협병.병주적전형증상표현위협맥황화,재협편정면형성망락상,재협배면협맥종대돌기명현,병주유협소차향하권곡,심지정편유협황화.식주조기피감염표현왜화.재발생황맥곡협병적황추규전곤,기병주솔고체60%이상.용연분슬전쌍생병독간병인물대수궤채집적병양진행PCR검측,종저사병양중균능확증출1조예기대소위570 bp적특이편단;기인극륭급측서분석결과표명,여해특이편단동원적균속쌍생병독과채두금색화협병독속병독DNA,기중여목이탄면화곡협병독(Cotton leaf curl Muhan virus,CLCuMV)분리물G6상사성최고,위99%.저사연구결과표명,엄동황추규황맥곡협병중존재연분슬전쌍생병독,해병해가능야시유CLCuMV침염인기적.
Okra is a vegetable crop introduced from Japan and Taiwan of China.Recently,the okra yellow vein leaf disease(OYVLD)has been found in Guangdong.The characteristic symptoms of the disease were yellow vein.interwoven network of yellow veins on the surface of leaves and obvious vein swelling on the back surface of Ieaves.The young leaves of the diseased plants were curling and their size Was reduced.In extreme cases,the infected leaves became totally yellow.Plants infected during the early stages remained stunted.In the fields,the rate of diseased plants Was more than 60%.Using the degenerate primers of whitefly-transmitted geminiviruses,the diseased samples collected randomly from fields were detected by PCR.The results showed that the expected 570 bp specific fragment Was amplified from all diseased samples.The specific fragment Was cloned and sequenced.BLAST results showed that all sequences homologous to the specific fragment belonged to the genus Begomovirus of the family Geminiviridae.and there was the highest similarity(99%)between the sequence of the specific fragment and DNA-A of Cotton leaf curl Multan virus(CLCuMV)isolate G6.These findings suggested that there were whitefly-transmitted geminiviruses in OYVLD samples from Guangdong,and the OYVLD was probably caused by CLCuMV.