CAJ | 학술논문

采用Sephadex G-25、Sephadex G-15葡聚糖凝胶柱层析法对鸡卵清蛋白多肽进行纯化.实验结果表明,鸡卵清蛋白多肽经Sephadex G-25凝胶柱层析分离出7个组分,其中组分3抗氧化活性最高,对DPPH自由基的清除率达到63.80%.组分3经Sephadex G-15凝胶柱层析,蒸馏水洗脱,得到4个组分,其中组分3-1和3-2具有较强的DPPH自由基清除能力,清除率分别为84.02%和81.17%.反相高效液相色谱图显示,组分3-1主要有一种成分.
채용Sephadex G-25、Sephadex G-15포취당응효주층석법대계란청단백다태진행순화.실험결과표명,계란청단백다태경Sephadex G-25응효주층석분리출7개조분,기중조분3항양화활성최고,대DPPH자유기적청제솔체도63.80%.조분3경Sephadex G-15응효주층석,증류수세탈,득도4개조분,기중조분3-1화3-2구유교강적DPPH자유기청제능력,청제솔분별위84.02%화81.17%.반상고효액상색보도현시,조분3-1주요유일충성분.
The peptides derived from egg white proteins were fractionated on Sephadex G-25 gel filtration column and the fraction were obtained by Sephadex G-25 gel filtration chromatography and fraction 3 showed the highest antioxidant activity with a subfraction 3-1 and 3-2 showed higher DPPH radical scavenging activities and the DPPH radical scavenging rates at 3 mg/ml were 84.02% and 81.17%, respectively. According to RP-HPLC chromatogram subfraction 3-1 had a high homogeneous composition.