CAJ | 학술논문

野大豆具有耐盐碱、抗寒、抗病等特点，对于研究大豆遗传基因的变迁、改善大豆品质和产量具有重要作用。为了探明黄河三角洲地区野大豆根瘤菌的生物学特性及其遗传多样性，从黄河三角洲地区的14个乡镇26个采样点采集了227株野大豆，从其根瘤中分离纯化根瘤茵，进行一系列的生理生化实验、抗逆性实验、结瘤能力测定、结瘤广谱性测定、16SrDNA序列测定和RAPD分析，共分离纯化出100株根瘤菌，其中菌株3D-21，3D．24，3K．8，3K．23VS等对酸、碱、盐、抗生素、高温、低温均有较强耐受性，且结瘤能力也较强；菌株7K-8结瘤能力较强，K-5抗逆性较强。16SrDNA序列测定表明所获菌株分属于3个属7个种，相近菌株的RAPD分析呈现明显的多态性。结果表明黄河三角洲地区野大豆根瘤茵存在着丰富的多样性，部分菌株结瘤能力和／或抗逆能力强，该研究为挖掘、利用优良菌株资源奠定了基础。
야대두구유내염감、항한、항병등특점，대우연구대두유전기인적변천、개선대두품질화산량구유중요작용。위료탐명황하삼각주지구야대두근류균적생물학특성급기유전다양성，종황하삼각주지구적14개향진26개채양점채집료227주야대두，종기근류중분리순화근류인，진행일계렬적생리생화실험、항역성실험、결류능력측정、결류엄보성측정、16SrDNA서렬측정화RAPD분석，공분리순화출100주근류균，기중균주3D-21，3D．24，3K．8，3K．23VS등대산、감、염、항생소、고온、저온균유교강내수성，차결류능력야교강；균주7K-8결류능력교강，K-5항역성교강。16SrDNA서렬측정표명소획균주분속우3개속7개충，상근균주적RAPD분석정현명현적다태성。결과표명황하삼각주지구야대두근류인존재착봉부적다양성，부분균주결류능력화／혹항역능력강，해연구위알굴、이용우량균주자원전정료기출。
Wild soybeans （ Glycine soja） have such positive characteristics as saline-alkali resistance, cold tolerance and disease resistance, so they play a significant role in the research on soybean gene evolution and the improvement of soybean quality and yield. We collected 227 wild soybeans from 26 sites of 14 rural towns in the Yellow River Delta, and isolated and purified rhizobia strains from wild soybean nodules. We then performed physiological and biochemical experiments, stress tolerance experiments, nodulation ability determination, nodulation wide spectrum analysis, 16S rDNA sequence determination and RAPD analysis. We isolated and purified 100 rhizobia strains from Glycine soja. Strains 3D-21, 3D-24, 3K-8 and 3K-23VS had strong tolerance against acid, alkali, salt, antibiotics, high and tow temperature and stronger nodulation capability. Strain 7K-8 had stronger nodulation capability and strain K-5 had stronger stress tolerance capability. 16S rDNA sequence analysis shows that.all the isolated strains belong to 3 genera and 7 species, and that RAPD analysis of similar strains exhibits obvious polymorphism. Results indicate that rich diversity exists in the rhizobia of wild soybean in the Yellow River Delta. Some strains have strong nodulation ability and/or stress tolerance ability. This research lays a foundation for the excavation and utilization of better strain resources.