中华放射医学与防护杂志
中華放射醫學與防護雜誌
중화방사의학여방호잡지
Chinese Journal of Radiological Medicine and Protection
2008年
2期
124-127
,共4页
祝宝让%蔡建明%唐古生%李百龙%高福%赵芳%崔建国%刘汉臣
祝寶讓%蔡建明%唐古生%李百龍%高福%趙芳%崔建國%劉漢臣
축보양%채건명%당고생%리백룡%고복%조방%최건국%류한신
反义STAT3%A549%辐射敏感性%凋亡%肿瘤治疗
反義STAT3%A549%輻射敏感性%凋亡%腫瘤治療
반의STAT3%A549%복사민감성%조망%종류치료
STAT3 antisense oligodeoxynucleotides%A549%Radiosensitivity%Apoptosis%Tumor therapy
目的 探讨高效反义STAT3转染肺腺癌A549细胞后,肿瘤细胞辐射敏感性的变化,为提高恶性肿瘤的辐射敏感性提供新的探索思路和途径.方法 用自行设计成功的高效反义STAT3(AS10)转染A549细胞后,以不同剂量γ射线照射,通过CCK-8试剂盒检测细胞增殖变化,Hoechst33258染色对细胞凋亡作形态学上的观察;用Annexin V/PI复染,流式细胞仪检测细胞早期凋亡率的变化;Western blot检测STAT3蛋白表达及磷酸化变化情况,以及其下游基因表达变化情况.结果 高效反义STAT3(AS10)转染后,加以γ射线照射,A549细胞的增殖相比于单独作用组受到明显抑制,细胞早期凋亡水平也增加,STAT3蛋白及其下游Bcl-Xl、Cyclin D1蛋白表达变化明显下降,STAT3蛋白磷酸化水平也降低.结论 反义STAT3(AS10)联合γ射线对A549细胞的增殖抑制和诱导凋亡作用明显增强,提高了A549细胞的辐射敏感性;表明阻断STAT3蛋白表达可能成为一种新的提高肿瘤辐射敏感性的有效手段.
目的 探討高效反義STAT3轉染肺腺癌A549細胞後,腫瘤細胞輻射敏感性的變化,為提高噁性腫瘤的輻射敏感性提供新的探索思路和途徑.方法 用自行設計成功的高效反義STAT3(AS10)轉染A549細胞後,以不同劑量γ射線照射,通過CCK-8試劑盒檢測細胞增殖變化,Hoechst33258染色對細胞凋亡作形態學上的觀察;用Annexin V/PI複染,流式細胞儀檢測細胞早期凋亡率的變化;Western blot檢測STAT3蛋白錶達及燐痠化變化情況,以及其下遊基因錶達變化情況.結果 高效反義STAT3(AS10)轉染後,加以γ射線照射,A549細胞的增殖相比于單獨作用組受到明顯抑製,細胞早期凋亡水平也增加,STAT3蛋白及其下遊Bcl-Xl、Cyclin D1蛋白錶達變化明顯下降,STAT3蛋白燐痠化水平也降低.結論 反義STAT3(AS10)聯閤γ射線對A549細胞的增殖抑製和誘導凋亡作用明顯增彊,提高瞭A549細胞的輻射敏感性;錶明阻斷STAT3蛋白錶達可能成為一種新的提高腫瘤輻射敏感性的有效手段.
목적 탐토고효반의STAT3전염폐선암A549세포후,종류세포복사민감성적변화,위제고악성종류적복사민감성제공신적탐색사로화도경.방법 용자행설계성공적고효반의STAT3(AS10)전염A549세포후,이불동제량γ사선조사,통과CCK-8시제합검측세포증식변화,Hoechst33258염색대세포조망작형태학상적관찰;용Annexin V/PI복염,류식세포의검측세포조기조망솔적변화;Western blot검측STAT3단백표체급린산화변화정황,이급기하유기인표체변화정황.결과 고효반의STAT3(AS10)전염후,가이γ사선조사,A549세포적증식상비우단독작용조수도명현억제,세포조기조망수평야증가,STAT3단백급기하유Bcl-Xl、Cyclin D1단백표체변화명현하강,STAT3단백린산화수평야강저.결론 반의STAT3(AS10)연합γ사선대A549세포적증식억제화유도조망작용명현증강,제고료A549세포적복사민감성;표명조단STAT3단백표체가능성위일충신적제고종류복사민감성적유효수단.
Objective To investigate the influence of effective STAT3 antisense oligodeoxynucleotides (AS10)on radiosensitivity of A549 cells.Methods Oligodeoxynucleotides were tansfected into A549 cells.Cell viability was assessed using CCK-8 assay.Apoptotic assay was performed using Hoechst 33258 staining and Annexin V/PI with FACS analysis,the expressions of STAT3,P-STAT3,Bcl-Xl and Cyclin D1,were measured by Western blot.Results The expression of STAT3、P-STAT3、Bcl-Xl and Cyclin D1 were downregulated.Cell viability was decreased significantly in those groups transfected with AS10 followed by different dose of γirradiation.Fraction of early apoptosis was increased when combined AS10 transfection with irradiation compared with those ceHs treated with irradiation alone or NS transfection with subsequent exposure to irradiation.Conclusion These results indicate that effective STAT3 antisense oligodeoxynucleotides(AS10)can enhance the therapeutic effect of γ-irradiation to A549 cells,which support the role of STAT3 as a potential molecular target for tumor therapies,and also a potential molecular target for enhancement of radiosensitivity of radioresistant tumors.
