中华临床感染病杂志
中華臨床感染病雜誌
중화림상감염병잡지
CHINESE JOURNAL OF CLINICAL INFECTIOUS DISEASES
2011年
4期
223-226
,共4页
钟家禹%朱冰%邓力%周名秀%陈翊%谢嘉慧%张莹莹%邝璐%肖密丝
鐘傢禹%硃冰%鄧力%週名秀%陳翊%謝嘉慧%張瑩瑩%鄺璐%肖密絲
종가우%주빙%산력%주명수%진익%사가혜%장형형%광로%초밀사
副黏病毒科感染%人偏肺病毒%流行病学%基因型
副黏病毒科感染%人偏肺病毒%流行病學%基因型
부점병독과감염%인편폐병독%류행병학%기인형
Paramyxoviridae infections%Human metapneumovirus%Epidemiology%Genotype
目的 了解广州地区婴幼儿患者呼吸道中人偏肺病毒(hMPV)感染的分子流行病学特点.方法 采集2010年1-12月到广州市妇女儿童医疗中心就诊的呼吸道感染患儿的咽拭子等样本1840份,对临床样本进行hMPV检测,对F基因进行扩增,并对PCR产物进行测序.结果 1 840份临床样本中66份hMPV检测阳性,阳性率为3.59％.全年除9、10月份外每月均有hMPV检出,其中4月份检出阳性率最高,为6.09％.随机选取3株hMPV,将其F基因核苷酸序列与A1、A2、B1、B2、C型和2008年分离的hMPVgz01株的F基因序列进行Clustal W比较,发现与A2b组的BJ1887同源性最高,为97％,4株广州分离的病毒株之间F基因的同源性为99％.序列与进化树分析表明广州地区流行的是A2b型.结论 2010年广州地区hMPV流行季节是春夏季,广州地区流行株的型别为A2b亚型.
目的 瞭解廣州地區嬰幼兒患者呼吸道中人偏肺病毒(hMPV)感染的分子流行病學特點.方法 採集2010年1-12月到廣州市婦女兒童醫療中心就診的呼吸道感染患兒的嚥拭子等樣本1840份,對臨床樣本進行hMPV檢測,對F基因進行擴增,併對PCR產物進行測序.結果 1 840份臨床樣本中66份hMPV檢測暘性,暘性率為3.59％.全年除9、10月份外每月均有hMPV檢齣,其中4月份檢齣暘性率最高,為6.09％.隨機選取3株hMPV,將其F基因覈苷痠序列與A1、A2、B1、B2、C型和2008年分離的hMPVgz01株的F基因序列進行Clustal W比較,髮現與A2b組的BJ1887同源性最高,為97％,4株廣州分離的病毒株之間F基因的同源性為99％.序列與進化樹分析錶明廣州地區流行的是A2b型.結論 2010年廣州地區hMPV流行季節是春夏季,廣州地區流行株的型彆為A2b亞型.
목적 료해엄주지구영유인환자호흡도중인편폐병독(hMPV)감염적분자류행병학특점.방법 채집2010년1-12월도엄주시부녀인동의료중심취진적호흡도감염환인적인식자등양본1840빈,대림상양본진행hMPV검측,대F기인진행확증,병대PCR산물진행측서.결과 1 840빈림상양본중66빈hMPV검측양성,양성솔위3.59％.전년제9、10월빈외매월균유hMPV검출,기중4월빈검출양성솔최고,위6.09％.수궤선취3주hMPV,장기F기인핵감산서렬여A1、A2、B1、B2、C형화2008년분리적hMPVgz01주적F기인서렬진행Clustal W비교,발현여A2b조적BJ1887동원성최고,위97％,4주엄주분리적병독주지간F기인적동원성위99％.서렬여진화수분석표명엄주지구류행적시A2b형.결론 2010년엄주지구hMPV류행계절시춘하계,엄주지구류행주적형별위A2b아형.
Objective To conduct a molecular epidemiological study on human metapneumovirus (hMPV) among pediatric patients in Guangzhou. Methods A total of 1 840 clinical specimens were obtained from pediatric patients with respiratory infections in Guangzhou Women and Children' s Medical Center in 2010. hMPV was detected by real-time TaqMan RT-PCR in clinical specimens. F gene was amplified and the PCR-products were directly sequenced. Results In 1 840 clinical specimens, 66 werehMPV-positive with a positive rate of 3.59％. hMPV was detected in all specimens except those collected in September and October, and the highest positive hMPV rate occurred in April (6.09％). The F genes of 3 randomly selected strains and hMPVgz01 ( isolated in 2008) were compared with subgroups A1, A2, B1,B2 and C, and the highest homology was with BJ1887 strain of genotype A2b (97％). The F genes of the randomly selected strains and hMPVgz01 were 99％ identical to each other. Sequences and phylogenetics analysis revealed that the epidemical strain in Guangzhou belonged to genotype A2b. Conclusion hMPV is prevalent in spring and summer among children in Guangzhou, and A2b is the predominant genotype.
