中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2010年
5期
324-326
,共3页
马江涛%陈慧%马学旻%关光玉%郝琼%温秋芳%孙晓强%杨东智%吴忠兰%詹军%檀晓娟%许文波
馬江濤%陳慧%馬學旻%關光玉%郝瓊%溫鞦芳%孫曉彊%楊東智%吳忠蘭%詹軍%檀曉娟%許文波
마강도%진혜%마학민%관광옥%학경%온추방%손효강%양동지%오충란%첨군%단효연%허문파
肠道病毒属%手足口病%基因
腸道病毒屬%手足口病%基因
장도병독속%수족구병%기인
Enterovirus%Hand,foot and mouth disease%Genes
目的 分析2009年宁夏回族自治区手足口病(Hand-Foot-and-Mouth Disease,HFMD)患者中肠道病毒71型(Enterovirus 71,EV71)分离株的基因特征.方法 2009年,从宁夏自治区344例HFMD病例中采集的385份标本,用人横纹肌肉瘤(Human Rhabdomyosarcoma,RD)细胞对标本进行肠道病毒分离培养;采用型特异性RT-PCR对阳性分离物中的EV71病毒进行鉴定;对鉴定的EV71毒株VP1编码区进行核苷酸序列测定分析.结果 共分离到肠道病毒126株,其中58株为EV71(46%),对其中46株EV71的VP1编码区序列进行测定和分析.宁夏分离株与EV71的A和B基因型代表株核苷酸序列同源性分别为81.7%～82.8%和83.1%～85.2%,氨基酸序列同源性分别为93.9%～95.9%和96.2%～97.9%;与C基因型的C1、C2、C3、C4a、C4b和C5亚型代表株核苷酸序列同源性分别为88.3%～90.6%、88.3%～90.1%、87.8%～89.0%、94.2%～98.9%、91.8%～94.1%和86.7%～89.1%;氨基酸序列同源性分别为97.9%～99.6%、97.9%～99.3%、97.6%～98.9%、97.9%～100%、98.6%～99.6%和97.9%～98.9%.在系统发生树上,这46株毒株与C4基因型代表株聚为一簇,属于C4亚型中的C4a分支.结论 2009年EV71的C4a亚型在宁夏有较广泛的流行,是宁夏流行的绝对优势基因亚型,C4a也是我国2005年以来流行的优势基因亚型.
目的 分析2009年寧夏迴族自治區手足口病(Hand-Foot-and-Mouth Disease,HFMD)患者中腸道病毒71型(Enterovirus 71,EV71)分離株的基因特徵.方法 2009年,從寧夏自治區344例HFMD病例中採集的385份標本,用人橫紋肌肉瘤(Human Rhabdomyosarcoma,RD)細胞對標本進行腸道病毒分離培養;採用型特異性RT-PCR對暘性分離物中的EV71病毒進行鑒定;對鑒定的EV71毒株VP1編碼區進行覈苷痠序列測定分析.結果 共分離到腸道病毒126株,其中58株為EV71(46%),對其中46株EV71的VP1編碼區序列進行測定和分析.寧夏分離株與EV71的A和B基因型代錶株覈苷痠序列同源性分彆為81.7%～82.8%和83.1%～85.2%,氨基痠序列同源性分彆為93.9%～95.9%和96.2%～97.9%;與C基因型的C1、C2、C3、C4a、C4b和C5亞型代錶株覈苷痠序列同源性分彆為88.3%～90.6%、88.3%～90.1%、87.8%～89.0%、94.2%～98.9%、91.8%～94.1%和86.7%～89.1%;氨基痠序列同源性分彆為97.9%～99.6%、97.9%～99.3%、97.6%～98.9%、97.9%～100%、98.6%～99.6%和97.9%～98.9%.在繫統髮生樹上,這46株毒株與C4基因型代錶株聚為一簇,屬于C4亞型中的C4a分支.結論 2009年EV71的C4a亞型在寧夏有較廣汎的流行,是寧夏流行的絕對優勢基因亞型,C4a也是我國2005年以來流行的優勢基因亞型.
목적 분석2009년저하회족자치구수족구병(Hand-Foot-and-Mouth Disease,HFMD)환자중장도병독71형(Enterovirus 71,EV71)분리주적기인특정.방법 2009년,종저하자치구344례HFMD병례중채집적385빈표본,용인횡문기육류(Human Rhabdomyosarcoma,RD)세포대표본진행장도병독분리배양;채용형특이성RT-PCR대양성분리물중적EV71병독진행감정;대감정적EV71독주VP1편마구진행핵감산서렬측정분석.결과 공분리도장도병독126주,기중58주위EV71(46%),대기중46주EV71적VP1편마구서렬진행측정화분석.저하분리주여EV71적A화B기인형대표주핵감산서렬동원성분별위81.7%～82.8%화83.1%～85.2%,안기산서렬동원성분별위93.9%～95.9%화96.2%～97.9%;여C기인형적C1、C2、C3、C4a、C4b화C5아형대표주핵감산서렬동원성분별위88.3%～90.6%、88.3%～90.1%、87.8%～89.0%、94.2%～98.9%、91.8%～94.1%화86.7%～89.1%;안기산서렬동원성분별위97.9%～99.6%、97.9%～99.3%、97.6%～98.9%、97.9%～100%、98.6%～99.6%화97.9%～98.9%.재계통발생수상,저46주독주여C4기인형대표주취위일족,속우C4아형중적C4a분지.결론 2009년EV71적C4a아형재저하유교엄범적류행,시저하류행적절대우세기인아형,C4a야시아국2005년이래류행적우세기인아형.
Objective To analyze the genetic characteristics of EV71 strains isolated from HFMD cases in Ningxia Hui Autonomous Region in 2009. Methods In 2009, totally 385 specimens from 344 HFMD cases were collected from Ningxia. Enterovirus isolation was performed in RD cell line from all the specimens. EV71 isolates were identified by specific RT-PCR from the positive cultures, and sequences of complete EV71 VP1 encoding region were determined for farther analyses. Results Totally from 126 EV strains isolated in this study, 58 EV71 strains (46%) were identified. And complete VP1 sequences of 46 EV71 strains were determined, and genetic analyses were performed. It was showed that the nucleotide identity of 46 Ningxia strains with the representatives of A and B genotypes were 81.7%-82.8% and 83. 1%-85.2%, and the amino acid identity were 93.9%-95.9% and 96. 2% -97.9% respectively. The nucleotide identity of NingXia EV71 isolates with representatives of subgenotype C1 ,C2, C3, C4a, C4b, and C5 were 88.3%-90.6% (97.9%-99.6%), 88.3%-90. 1% (97.9%-99.3%), 87.8%-89.0%(97.6% -98.9% ), 94. 2% -98. 9% ( 97. 9% -100% ), 91.8% -94. 1% ( 98.6% -99. 6% ), and 86. 7% -89. 1% (97.9%-98.9% ). Phylogenetic tree analysis revealed that 46 stains were clustered with reference stains of subgenotype C4 and the Ningxia EV71 isolates were belonged to subgenotype C4a. Conclusion EV71 of subgenotype C4a had spread widely in Ningxia in 2009, which was absolutely predominant type in Ningxia in 2009 and also as the predominant type in China mainland since 2005.
